165,307 results

1. pXPR_502

   Plasmid

   #96923

   Purpose

   for CRISPRa, lentiviral expression of gRNA scaffold using activator P65 HSF

   Depositor

   John Doench, David Root

   Article

   Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti6.3-sRGN3.1-BlastR

   Plasmid

   #220964

   Purpose

   Expresses human codon-optimized sRGN3.1

   Depositor

   Hyongbum Kim

   Article

   Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

   Insert

   sRGN3.1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT3EF1aH-myr-Akt

   Plasmid

   #179909

   Purpose

   This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used with Cre for in vivo studies.

   Depositor

   Xin Chen

   Article

   Ho et al Hepatology. 2012 Mar;55(3):833-45. doi:

   Insert

   Akt1 (Akt1 Mouse)
   

   Tags

   HA and Myr

   Expression

   Mammalian

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEVOL-pylT-N346A/C348A

   Plasmid

   #127411

   Purpose

   Double mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethers

   Depositor

   Wenshe Liu

   Article

   Wang et al J Am Chem Soc. 2012 Feb 15;134(6):2950-3

   Insert

   Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
   

   Expression

   Bacterial

   Mutation

   N346A-C348A

   Promoter

   araBAD

   Available Since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-C1 F-tractin-EGFP

   Plasmid

   #58473

   Purpose

   Expresses a cytoplasmic actin filament reporter, the neuronal inositol 1,4,5-triphosphate 3-kinase A actin-binding domain known as F-tractin, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Bioarchitecture. 2015 Aug 28:1-14.

   Insert

   F-tractin (Itpka Rat)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDSW1728

   Plasmid

   #120812

   Purpose

   E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt)

   Depositor

   Craig Ellermeier, David S. Weiss

   Article

   Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1

   Insert

   red fluorescent protein (mCherry), codon-optimized for C. difficile
   

   Expression

   Bacterial

   Promoter

   Ptet

   Available Since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-21a_3xNLS_SpCas9_protein_expression

   Plasmid

   #114365

   Purpose

   Protein expression plasmid for 3xNLS SpCas9 in pET-21a backbone

   Depositor

   Scot Wolfe

   Article

   Wu et al Nat Med. 2019 May;25(5):776-783. doi: 10

   Insert

   3xNLS_SpCas9
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV4-BlaM-Vpr

   Plasmid

   #21950

   Depositor

   Warner Greene

   Article

   Cavrois et al Nat Biotechnol. 2002 Nov . 20(11):1151-4

   Insert

   BlaM-Vpr (vpr Fusion of BlaM and HIV-1 Vpr)
   

   Expression

   Mammalian

   Available Since

   Oct. 1, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pLenti6.3-SlugCas9-BlastR

   Plasmid

   #220965

   Purpose

   Expresses human codon-optimized SlugCas9

   Depositor

   Hyongbum Kim

   Article

   Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

   Insert

   SlugCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. mNeonGreen-Giantin

    Plasmid

    #98880

    Purpose

    In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    Giantin
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-TRE-EGFP

    Plasmid

    #89875

    Purpose

    EGFP reporter vector of iTango system for AAV production

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    tetO-EGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    tetO

    Available Since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLAMP1-mCherry

    Plasmid

    #45147

    Depositor

    Amy Palmer

    Article

    Van Engelenburg et al Nat Methods. 2010 Apr;7(4):325-30. doi:

    Insert

    LAMP1-mCherry (LAMP1 Human, Synthetic)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-CAG-jGCaMP8s-WPRE

    Plasmid

    #179256

    Purpose

    AAV-mediated expression of GCaMP8s under the CAG promoter

    Depositor

    Loren Looger

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP8s
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKM-T4-CMV-TnpB-optimized-P2A-GFP-Puro

    Plasmid

    #205631

    Purpose

    Expression of TnpBmax in mammalian cells

    Depositor

    Gerald Schwank

    Article

    Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

    Insert

    ISDra2-TnpB optimized for mammalian expression
    

    Tags

    NLS-3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-KIT-V5/HIS

    Plasmid

    #201986

    Purpose

    expression of human KIT receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    KIT receptor tyrosine kinase (KIT Human)
    

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. rtTA-N144

    Plasmid

    #66810

    Purpose

    2nd generation lentiviral transfer plasmid. Expresses the reverse tetracycline-controlled transactivator (rtTA) with hygromycin resistance

    Depositor

    Andrew Yoo

    Article

    Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi

    Insert

    Reverse tetracycline-controlled transactivator
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Human EF1a

    Available Since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRGEB32

    Plasmid

    #63142

    Purpose

    Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation.

    Depositor

    Yinong Yang

    Article

    Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    Rice snoRNA U3 promoter for gRNA/PTG expression a…

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag p21 WT

    Plasmid

    #16240

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Mar . 3(3):245-52.

    Insert

    p21 (CDKN1A Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmGFP-P2A-K0-P2A-RFP

    Plasmid

    #105686

    Purpose

    Double fluorescent stalling reporter K0

    Depositor

    Ramanujan Hegde

    Article

    Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

    Inserts

    P2A-3xFLAG-VHPbeta
    K0
    P2A-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV (from vector)

    Available Since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPB-cT3G-cERP2-NR5A1

    Plasmid

    #192915

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of NR5A1

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    NR5A1 (NR5A1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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