We narrowed to 6,934 results for: crispr cas9 plasmids

1. pSQT817

   Plasmid

   #53373

   Purpose

   wildtype Cas9 expression plasmid

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJA42

   Plasmid

   #59930

   Purpose

   gRNA for cleavage at rol-6(su1006) locus in C elegans

   Depositor

   Andrew Fire

   Article

   Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

   Insert

   rol-6(su1006) gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Sept. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. peft-3::cas-9::tbb-2 3'UTR

   Plasmid

   #48960

   Purpose

   this plasmid contains the codon optimized gene encoding Cas9 for C. elegans.

   Depositor

   Mario de Bono

   Article

   Chen et al Nucleic Acids Res. 2013 Sep 5.

   Insert

   cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   eef-1A.1 (eft-3)

   Available since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJA50

   Plasmid

   #59931

   Purpose

   gRNA for cleavage at unc-58(e665) locus in C elegans

   Depositor

   Andrew Fire

   Article

   Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

   Insert

   unc-58(e665) gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Sept. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJA54

   Plasmid

   #60212

   Purpose

   cleavage near e1350 locus in sqt-1

   Depositor

   Andrew Fire

   Article

   Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

   Insert

   sqt-1(e1350) gRNA1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJA55

   Plasmid

   #59935

   Purpose

   gRNA for cleavage near sqt-1(e1350) locus in C elegans

   Depositor

   Andrew Fire

   Article

   Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

   Insert

   sqt-1(e1350) gRNA2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Sept. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJA59

   Plasmid

   #59932

   Purpose

   gRNA for cleavage at unc-109(n499) locus in C elegans

   Depositor

   Andrew Fire

   Article

   Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

   Insert

   unc-109(n499) gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Sept. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJA14

   Plasmid

   #59929

   Purpose

   gRNA for cleavage at rde-1(H974) locus in C elegans

   Depositor

   Andrew Fire

   Article

   Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

   Insert

   rde-1(H974) gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Sept. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJA46

   Plasmid

   #59928

   Purpose

   gRNA for cleavage at rde-1(D801) locus in C elegans

   Depositor

   Andrew Fire

   Article

   Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

   Insert

   rde-1(D801) gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJA45

    Plasmid

    #59927

    Purpose

    gRNA for cleavage at rde-1(D718) locus in C elegans

    Depositor

    Andrew Fire

    Article

    Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

    Insert

    rde-1(D718) gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. LRG2.1-CyOFP1

    Plasmid

    #124771

    Purpose

    3rd generation lentiviral plasmid to co-express a guide RNA and CyOFP1

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. LRG2.1-mOrange

    Plasmid

    #124772

    Purpose

    3rd generation lentiviral plasmid to co-express a guide RNA and mOrange

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAEF6

    Plasmid

    #136303

    Purpose

    pAEF5 plasmid with two gRNA inserted

    Depositor

    Gilles Fischer

    Article

    Agier et al Methods Mol Biol. 2021;2196:181-198. doi

    Insert

    double cassette coding for gRNA
    

    Use

    CRISPR; Template for pcr to insert double grna co…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPomyces-2

    Plasmid

    #61737

    Purpose

    Streptomyces expression of codon-optimized Cas9 and custom gRNA

    Depositor

    Huimin Zhao

    Article

    Cobb et al ACS Synth Biol. 2014 Dec 8.

    Inserts

    sSpCas9
    gRNA cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    BbsI-flanked lacZ cassette inserted in place of s…

    Promoter

    gapdhp(EL) and rpsL(XC)-BbsI

    Available since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNM220_AAVS1 integration helper

    Plasmid

    #211864

    Purpose

    Cas9 cutting at AAVS1 safe harbor locus

    Depositor

    Iain Cheeseman

    Insert

    AAVS1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. CRISPseq-BFP-backbone

    Plasmid

    #85707

    Purpose

    Backbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with a BFP fluorescent marker. Does NOT include Cas9.

    Depositor

    Ido Amit

    Article

    Jaitin et al Cell. 2016 Dec 15;167(7):1883-1896.e15.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. CRISPseq-mCherry-backbone

    Plasmid

    #85708

    Purpose

    Backbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with an mCherry fluorescent marker. Does NOT include Cas9.

    Depositor

    Ido Amit

    Article

    Jaitin et al Cell. 2016 Dec 15;167(7):1883-1896.e15.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. sgRNA1_NANOG

    Plasmid

    #64153

    Purpose

    Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1_NANOG (NANOG Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. sgRNA4_NANOG

    Plasmid

    #64156

    Purpose

    Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA4_NANOG (NANOG Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. sgRNA3_NANOG

    Plasmid

    #64155

    Purpose

    Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA3_NANOG (NANOG Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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