We narrowed to 5,822 results for: pcas

1. pBW1604_pCAG-PV1-PYL-L1-PhiC31-234C-BGHpA

   Plasmid

   #108798

   Purpose

   Expresses aa234 to C-terminus of PhiC31 integrase fused to PYL CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   PhiC31 integrase fragment aa234 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   NLS and PYL

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. BPK3258 - human expression plasmid for eSpCas9(1.1)

   Plasmid

   #101176

   Purpose

   Human expression plasmid for SpCas9 eSpCas9(1.1) variant: CMV-T7-hSpCas9-eSpCas9(1.1)(K848A, K1003A, R1060A)-NLS(SV40)-3xFLAG

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-eSpCas9(1.1)(K848A/K1003A/R1060A)
   

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   K848A/K1003A/R1060A

   Promoter

   CMV

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-hMb3Cas12a-NLS(nucleoplasmin)-3xHA (RTW2500)

   Plasmid

   #115142

   Purpose

   CAG promoter expression plasmid for human codon optimized Mb3Cas12a nuclease with C-terminal NLS and HA tag

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver, Keith Joung

   Article

   Marino et al Science. 2018 Sep 6. pii: science.aau517

   Insert

   human codon optimized Mb3Cas12a
   

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBW808_pCAG-PV5-VloxP-loxN-F3-GFP-BGHpA

   Plasmid

   #87584

   Purpose

   PV5 for 3-input, 2-output Full Adder

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   EGFP
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. NFATc2-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

   Plasmid

   #75232

   Purpose

   CRISPR/Cas9 plasmid against human NFATc2

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against NFATc2 (NFATC2 Human)
   

   Use

   CRISPR

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. NFATc1-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

   Plasmid

   #75236

   Purpose

   CRISPR/Cas9 plasmid against human NFATc1

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against NFATc1 (NFATC1 Human)
   

   Use

   CRISPR

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-GST-hFIP200(1363-1370Mut)-MBP

   Plasmid

   #190038

   Purpose

   Expression of FIP200 mutant

   Depositor

   James Hurley

   Article

   Shi et al Elife. 2020 Aug 10;9. pii: 59099. doi: 1

   Insert

   FIP200 (RB1CC1 Human)
   

   Tags

   GST and MBP

   Expression

   Mammalian

   Mutation

   1363 RDKDLIES 1370 to GSSGGSSG

   Promoter

   CMV

   Available Since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBW2656_pCAG-PV1-nMag-L1-CTD-ENTRY-BGHpA

   Plasmid

   #108849

   Purpose

   Insert protein fragment into KpnI/EcoRI to fuse to nMag tag on N-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   nMag

   Expression

   Mammalian

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. NFATc1-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

   Plasmid

   #75237

   Purpose

   CRISPR/Cas9 plasmid against human NFATc1

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against NFATc1 (NFATC1 Human)
   

   Use

   CRISPR

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-Flag-SpCas9-HF1 (without sgRNA)

    Plasmid

    #92102

    Purpose

    Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    Cbh

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pD649-HAsp-EPCAM-COMP5AP-AviTag-9xHis

    Plasmid

    #157257

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    EPCAM (EPCAM Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pD649-HAsp-EPCAM-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156693

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    EPCAM (EPCAM Human)
    

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS-mTagBFP2-T2A-sfGFP-iCre-ERT2

    Plasmid

    #128166

    Purpose

    Monomeric TagBFP2 and sfGFP-iCre-ERT2 recombinase (PAPGSTM N-terminus, unskippable linker)

    Depositor

    Mario Capecchi

    Article

    Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

    Insert

    mTagBFP2-T2A-sfGFP-iCre-ERT2
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. PET-SpCas9-HF1-plus-NLS-6xHis

    Plasmid

    #207381

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    SpCas9-HF1-plus
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, Q695A, Q926A, amino acids 1005-1013 replac…

    Promoter

    T7

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBW2067_pCAG-PV1-GAI-L1-CTD-ENTRY-BGHpA

    Plasmid

    #108845

    Purpose

    Insert protein fragment into KpnI/EcoRI to fuse to GAI tag on N-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    GAI

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBW2065_pCAG-PV1-GID1-L1-CTD-ENTRY-BGHpA

    Plasmid

    #108843

    Purpose

    Insert protein fragment into KpnI/EcoRI to fuse to GID1 tag on N-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    GID1

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCSDest2-SpCas9-NLS-3XHA-NLS-Zif268

    Plasmid

    #69219

    Purpose

    Expresses wild type SpCas9 fused to Zif268 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    Zif268 (Egr1 Mouse)
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSD36_pCAG-PV1-FKBP-L1-TP901-327C-BGHpA

    Plasmid

    #108814

    Purpose

    Expresses aa327 to C-terminus of TP901 integrase fused to FKBP

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    TP901 integrase fragment aa327 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-MBP-Foldon-ATG9 (830-839)

    Plasmid

    #188091

    Purpose

    To make ATG9 C-terminal tail trimer for expression in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG9 (ATG9A Human)
    

    Tags

    MBP tag

    Expression

    Mammalian

    Mutation

    ATG9 (830-839)

    Promoter

    CMV

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS4

    Plasmid

    #69227

    Purpose

    Expresses wild type SpCas9 fused to ZFP-TS4 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TS4 ZFP
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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