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We narrowed to 4,580 results for: NAP

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Showing: 3691 - 3720 of 4580 results

  2. str-KDEL_Stx5L(M55V)-SBP-mCitrine

    Plasmid
    #209846
    Purpose
    Synchronize trafficking of Stx5L (M55V) from the ER, for use with FLIM (RUSH system)
    Depositor
    Geert van den Bogaart
    Article
    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:
    Insert
    STX5 (STX5 Human)
    Tags
    Streptavidin Binding Protein (SBP) and mCitrine
    Expression
    Mammalian
    Mutation
    Changed Methionine 55 to Valine, causes loss of S…
    Promoter
    CMV
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    209846-plasmid-map-sequence-id-413647

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  3. pAAV-EGFP.T2A.NCLX.3'UTR

    Plasmid
    #181872
    Purpose
    Expresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoter (includes a large portion of the Slc8b1 3'UTR)
    Depositor
    Hilmar Bading
    Article
    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10
    Inserts
    solute carrier family 8 member B1 (Slc8b1 Mouse)
    EGFP
    3'UTR of solute carrier family 8 member B1
    Use
    AAV and Adenoviral
    Tags
    HA, T2A, and myc
    Expression
    Mammalian
    Mutation
    includes a large portion of the Slc8b1 3'UTR…
    Promoter
    synthetic hybrid CAG promoter
    Available Since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    181872-plasmid-map-sequence-id-356018

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  4. str-KDEL_Stx5S-SBP-mCitrine

    Plasmid
    #154845
    Purpose
    Synchronize trafficking of Stx5S from the ER, for use with FLIM (RUSH system)
    Depositor
    Geert van den Bogaart
    Article
    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:
    Insert
    Stx5 (Short isoform) (STX5 Human)
    Tags
    Streptavidin Binding Protein (SBP) and mCitrine
    Expression
    Mammalian
    Mutation
    Lacks amino acids 1-54, enocdes short isoform of …
    Promoter
    CMV
    Available Since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    154845-plasmid-map-sequence-id-325019

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  11. Spike Display_Part 1-5 DO

    Plasmid
    #172726
    Purpose
    Encodes a sfGFP dropout expression cassette in place of Parts 1-5 to be used in the Spike Display system
    Depositor
    IIya Finkelstein
    Article
    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e
    Insert
    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CMV
    Available Since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    172726-plasmid-map-sequence-id-335212

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  13. pcDNA5 rtTA BirA eIF4G +MIC

    Plasmid
    #158790
    Purpose
    pcDNA5 based transfection plasmid for the exogenous expression of N-terminal BirA-Flag-tagged eIF4G1 including the microexon for generation of N2A FlpIn cell lines for BioID
    Depositor
    Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1
    Insert
    eIF4G1 (with microexon)
    Tags
    3xFlag and BirA*
    Expression
    Mammalian
    Promoter
    miniCMV
    Available Since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    158790-plasmid-map-sequence-id-309812

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  19. CRISPRoff-mScarletI

    Plasmid
    #217783
    Purpose
    Expresses CRISPRoff-mScarletI (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-mScarletI-KRAB) downstream of the CAG promoter for gene epigenetic silencing
    Depositor
    Adriano Aguzzi
    Article
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    CRISPRoff-mScarletI (DNMT3A-DNMT3L-dCas9-mScarletI-KRAB)
    Tags
    2xNLS, DNMT3A-DNMT3L, HA, KRAB, and mScarletI
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    217783-plasmid-map-sequence-id-468886

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  20. pZB246-T7-T+

    Plasmid
    #250903
    Purpose
    Expresses N-terminal His-tag thermally stabilized T7 RNAP under a lac-inducible promoter on Kanamycin Resistance Backbone
    Depositor
    Timothy Whitehead
    Article
    Baumer et al bioRxiv [Preprint]. 2025 Nov 12:2025.11.
    Insert
    Thermally Stable T7 RNA Polymerase
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Arginine 31 to Aspartic Acid, Alanine 61 to Argin…
    Promoter
    pT5-lacUV
    Available Since
    Feb. 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    250903-plasmid-map-sequence-id-499452

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  26. pAAV.hSyn.FLP-FRT.iGluSnFR3.v857.SGZ

    Plasmid
    #196224
    Purpose
    Fluorescent reporter for glutamate, third generation, variant 857 in SGZ backbone. iGluSnFR3.v857.SGZ
    Depositor
    Kaspar Podgorski
    Article
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Insert
    pAAV hSyn FLP-FRT iGluSnFR3 v857.SGZ
    Use
    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…
    Tags
    IgK-chain, Myc epi tag, and Stargazin and PDZ dom…
    Expression
    Mammalian
    Available Since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    196224-plasmid-map-sequence-id-405478

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  27. pBFC1207

    Plasmid
    #186694
    Purpose
    VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End
    Depositor
    Jennifer Doudna
    Article
    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:
    Inserts
    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    Vc_2xBsaI_NT (Guide Stuffer) crRNA
    Tn7 transposon
    GmR+sfGFP VcDART Cargo
    SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
    Expression
    Bacterial
    Promoter
    PLac
    Available Since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    186694-plasmid-map-sequence-id-371385

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  28. pAAV-Syn-SomaGCaMP6f1

    Plasmid
    #158755
    Purpose
    Cell body-targeted GCaMP6f under synapsin promoter: GCaMP6f followed by a linker, the tail region of AnkyrinG, and an ER export motif (GCaMP6f-27-AnkTail-motif-ER2). Not as bright as SomaGCaMP6f2.
    Depositor
    Edward Boyden
    Article
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Insert
    SomaGCaMP6f1
    Use
    AAV
    Expression
    Mammalian
    Promoter
    synapsin promoter
    Available Since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158755-plasmid-map-sequence-id-310657

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  29. Spike Display_B.1.1.7

    Plasmid
    #172735
    Purpose
    Expression vector for SARS-CoV-2 (B.1.1.7) spike used in the Spike Display system
    Depositor
    IIya Finkelstein
    Article
    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e
    Insert
    SARS-CoV-2 S B.1.1.7 (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CMV
    Available Since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    172735-plasmid-map-sequence-id-340032

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  30. Spike Display_B.1.1.248

    Plasmid
    #172737
    Purpose
    Expression vector for SARS-CoV-2 (B.1.1.248) spike used in the Spike Display system
    Depositor
    IIya Finkelstein
    Article
    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e
    Insert
    SARS-CoV-2 S B.1.1.248 (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CMV
    Available Since
    Aug. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    172737-plasmid-map-sequence-id-339266

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Showing: 3691 - 3720 of 4580 results