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pcDNA3.1(-)-qmTQ2-ATP

Plasmid

#225248

Purpose

mammalian expression of qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-ATP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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p2CT-Cdi

Plasmid

#83044

Purpose

Expresses Corynebacterium diphtheriae Cas9 (CdiCas9)

Depositor

Jennifer Doudna

Article

Ma et al Mol Cell. 2015 Nov 5;60(3):398-407. doi:

Insert

CdiCas9

Tags

His-MBP

Expression

Bacterial

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only
pEC-K-CBP_CjeCas9

Plasmid

#83043

Purpose

Expresses Campylobacter jejuni Cas9 (Cje Cas9).

Depositor

Jennifer Doudna

Article

Ma et al Mol Cell. 2015 Nov 5;60(3):398-407. doi:

Insert

CjeCas9

Expression

Bacterial

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1mychisA XDH

Plasmid

#10958

Depositor

Toren Finkel

Article

Ohtsubo et al Circ Res. 2004 Nov 26. 95(11):1118-24.

Insert

xanthine dehydrogenase (XDH Human)

Tags

his and myc

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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U6>Control(F+E)

Plasmid

#60006

Purpose

U6 promoter driving control (F+E) sgRNA

Depositor

Lionel Christiaen

Article

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Insert

Control (F+E) sgRNA

Use

CRISPR

Promoter

Ciinte.U6

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pLZ12Km2-P23R:TA:fflucRT

Plasmid

#88901

Purpose

Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression

Depositor

Thomas Proft

Article

Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

Insert

toxin-antitoxin + Red-shifted firefly luciferase

Expression

Bacterial

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pX458-HypaCas9

Plasmid

#190604

Purpose

PX458 (Plasmid #48138) with the N692A, M694A, Q695A, and H698A mutations

Depositor

Yuichiro Miyaoka

Article

Takahashi et al iScience. 2022 Nov 17;25(12):105619. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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SCP1-CMVenh-luc

Plasmid

#22216

Depositor

James Kadonaga

Article

Juven-Gershon et al Nat Methods. 2006 Nov . 3(11):917-22.

Insert

SCP1 (super core promoter 1)-CMVenh luciferase

Use

Luciferase

Expression

Mammalian

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-Pcdh19-GFP

Plasmid

#35675

Depositor

James Jontes

Article

Biswas et al J Cell Biol. 2010 Nov 29;191(5):1029-41.

Insert

Pcdh19 (pcdh19 Zebrafish)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only
pJMP3665

Plasmid

#222351

Purpose

Expression vector with PabstBR promoter, hygR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

Depositor

Jason Peters

Article

Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013

Type

Empty backbone

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a LEU2

Plasmid

#61923

Purpose

Expression of K. lactis LEU2 cloned into pFA6a

Depositor

Jon Houseley, David Tollervey

Article

Houseley et al Nucleic Acids Res. 2011 Nov 1;39(20):877

Insert

LEU2

Use

Yeast genomic targeting

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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pAB213

Plasmid

#101677

Purpose

mCherry under T7 promoter control

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

mCherry under T7 promoter control

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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LV-Camk2-Ace-7aa-mScarlet

Plasmid

#129701

Purpose

Expresses Ace-7aa-mScarlet in mammalian cells

Depositor

Yiyang Gong

Article

Beck et al Sci Rep. 2019 Nov 4;9(1):15878. doi: 10.

Insert

Ace-7aa-mScarlet

Use

Lentiviral

Expression

Mammalian

Promoter

Camk2

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pC28-LIC-sfGFP-His

Plasmid

#165479

Purpose

pET based vector for protein expression in E.coli for targets fused with C-terminal superfolder GFP and His tag

Depositor

Renato Bruni

Article

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Tags

TEV-GFP-6xHis

Expression

Bacterial

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-6P-SEW-YC3.6

Plasmid

#31379

Depositor

Fritjof Helmchen

Article

Minderer et al J Physiol. 2011 Nov 14.

Insert

Yellow Cameleon 3.60

Use

AAV

Tags

Venus and eCFP

Available Since

Nov. 29, 2011

Availability

Academic Institutions and Nonprofits only

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p3E_afp_UTR

Plasmid

#199341

Purpose

pAMS448; Gateway 3' entry clone encoding ocean pout antifreeze (afp) protein 3'UTR

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

afp 3' UTR

Use

Gateway donor vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pMTU-DO-HIS-C4

Plasmid

#160340

Purpose

High-copy expression vector for K. marxianus, histidine selection

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pMTU-DO-LEU-C4

Plasmid

#160341

Purpose

High-copy expression vector for K. marxianus, leucine selection

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pTNT-pT7-tetR-mCherry

Plasmid

#140870

Purpose

T7 promoter driven synthesis of TetR-mCherry fusion protein

Depositor

Neal Devaraj

Article

Niederholtmeyer et al Nat Commun. 2018 Nov 28;9(1):5027. doi:

Insert

TetR-mCherry expressed with T7 promoter

Use

Synthetic Biology

Tags

mCherry

Expression

Bacterial

Promoter

T7

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAB167

Plasmid

#101672

Purpose

Opto-T7RNAP*(563), equal expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563), equal expr.

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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