We narrowed to 7,575 results for: Trac;
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Plasmid#134132PurposeBacterial expression of His-TEV-Ska1 and Ska2DepositorInsertHis-TEV-human Ska1 ; human Ska2
TagsHis-TEVExpressionBacterialMutationK183A K184AAvailable SinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMDJ15 - pEXP[Peft-3 | mCardinal (NLS) | tbb-2 UTR]
Plasmid#159801PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | mCardinal (NLS) | tbb-2 UTR]
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEM389 - mosTI unc-119 - NNN - mScarlet(nls) - tbb-2
Plasmid#182349PurposemScarlet transcriptional reporter cloning vector for mosTI(unc-119). Transgene inserted upstream of mScarlet(2xNLS) and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)DepositorTypeEmpty backboneExpressionWormAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-VAMP8
Plasmid#135553PurposeExpress mouse VAMP8 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertVAMP8
Tags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQUAST>stop>shibire (ts1)
Plasmid#24363DepositorInsertShibire (ts1) (shi Fly)
ExpressionInsectAvailable SinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
gRD21A-mRFP
Plasmid#181959PurposeRD21A protein tagged with mRFP, under control of native promoterDepositorInsertRD21A genomic sequence including 2kb upstream of the ATG
TagsmRFPExpressionPlantPromoterRD21A nativeAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2210 - [1-2] ENTR - Fluor - mMaple3(1 intron, syntrons(3), no_start, no_stop)
Plasmid#159864PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - mMaple3(1 intron, syntrons(3), no_start, no_stop)
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
CD4_LD 0_CS G_tTA-BFP
Plasmid#58869PurposeMESA target chain with CD4 ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with CD4 ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianPromoterCMVAvailable SinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMDJ16 - pEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR]
Plasmid#159802PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR]
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVH028
Plasmid#80393PurposeContains constitutive histidine kinase (pCon-Taz) with 4x SH3 scaffold domains + salicylate inducible phosphatase (Psal-CusSmut)DepositorUseSynthetic BiologyTagsFused by GS linkers to 4 SH3 domainsExpressionBacterialMutationGlycine 448 changed to Alanine, makes it primaril…Available SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2262A - [1-2] ENTR - Fluor - ce-mNeon (1 intron, syntrons(3), no_atg, no_stop)
Plasmid#159851PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-mNeon (1 intron, syntrons(3), no_atg, no_stop)
ExpressionWormMutationNot applicableAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSPNprM-hp
Plasmid#48120PurposeShuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein NprM under control of the xylose inducible promoterDepositorInsertsignal peptide of NprM
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMDJ13 - pEXP[Peft-3 | tagRFP-T (NLS) | tbb-2 UTR]
Plasmid#159800PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | tagRFP-T (NLS) | tbb-2 UTR]
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-CD4-Sema4D
Plasmid#51603Purposeexpresses Sema4D/CD4 chimera with intracellular Sema4D, extracellular CD4DepositorInsertCD4-Sema4D
TagsmycExpressionMammalianMutationSema4D amino acids 1-754 have been replaced with …PromoterCMVAvailable SinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2245 - [1-2] ENTR - Fluor - ce-tagBFP2 (1 intron, syntrons(3), no_atg, no_stop)
Plasmid#159843PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-tagBFP2 (1 intron, syntrons(3), no_atg, no_stop)
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry_LD 0_CS M_tTA-BFP
Plasmid#58874PurposeMESA target chain with mCherry ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
UseAAVTagsEBFPExpressionMammalianPromoterCMVAvailable SinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMT1100
Plasmid#8618DepositorInsertribonuclease Sa3, barstar
TagsphoA signal sequence to RNase Sa3ExpressionBacterialMutationIn addition to the substitution of the barnase ge…Available SinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
mCherry_LD 0_CS A_tTA-BFP
Plasmid#58873PurposeMESA target chain with mCherry ectodomain, no linker domain, A cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (A in P1')
UseAAVTagsEBFPExpressionMammalianPromoterCMVAvailable SinceNov. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pSPVpr-hp
Plasmid#48119PurposeShuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Vpr under control of the xylose inducible promoterDepositorInsertsignal peptide of Vpr
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only