We narrowed to 2,759 results for: 2-Oct

1. pET11a-STEPtag-L10-TAX

   Plasmid

   #159374

   Purpose

   Expresses STEPtag fused to the N-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.

   Depositor

   Roberto Chica

   Article

   Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

   Insert

   STEPtag-L10-TAX
   

   Use

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pB-puro-mU6-CNPshRNA2

   Plasmid

   #126612

   Purpose

   Expresses shRNA against human CNP under mouse U6 promoter

   Depositor

   Xiaozhong Wang

   Article

   Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

   Insert

   CNP ShRNA-2
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   Mouse U6 promoter

   Available since

   June 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK511

   Plasmid

   #71591

   Purpose

   Produces Thermoplasma acidophilum citrate synthase with C-terminal His6 tag, His222>Gln mutant (TpCSH6-H222Q)

   Depositor

   T. J. Kappock

   Article

   Murphy et al Acta Crystallogr F Struct Biol Commun. 2

   Insert

   citrate synthase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes histidine-222 to glutamine

   Promoter

   T7 promoter

   Available since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221-W279A-short-UBASH3A-Ctag

   Plasmid

   #195402

   Purpose

   Human W279A short-form UBASH3A in Gateway pDONR221 (for C-terminal tag)

   Depositor

   Patrick Concannon

   Article

   Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

   Insert

   UBASH3A (UBASH3A Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   W279A

   Promoter

   Available since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR221-K202R-short-UBASH3A-Ctag

   Plasmid

   #195399

   Purpose

   Human K202R short-form UBASH3A in Gateway pDONR221 (for C-terminal tag)

   Depositor

   Patrick Concannon

   Article

   Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

   Insert

   UBASH3A (UBASH3A Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   K202R

   Promoter

   Available since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. K202R-short-UBASH3A-V5-6xHis

   Plasmid

   #195406

   Purpose

   Mammalian expression of human K202R short-form UBASH3A with V5-6xHis tag

   Depositor

   Patrick Concannon

   Article

   Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

   Insert

   UBASH3A (UBASH3A Human)
   

   Use

   Tags

   V5-6xHis

   Expression

   Mammalian

   Mutation

   K202R

   Promoter

   Available since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. W279A-short-UBASH3A-V5-6xHis

   Plasmid

   #195409

   Purpose

   Mammalian expression of human W279A short-form UBASH3A with V5-6xHis tag

   Depositor

   Patrick Concannon

   Article

   Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

   Insert

   UBASH3A (UBASH3A Human)
   

   Use

   Tags

   V5-6xHis

   Expression

   Mammalian

   Mutation

   W279A

   Promoter

   Available since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDGO186N-KS2

   Plasmid

   #174302

   Purpose

   Replicating plasmid with sgRNA cassette containing a killing spacer #2 targeting the wild type polC gene.

   Depositor

   Lee Lynd

   Article

   Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

   Insert

   spacer expression cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pB-puro-mU6-RtcAshRNA2

   Plasmid

   #126614

   Purpose

   Expresses shRNA against human RtcA under mouse U6 promoter

   Depositor

   Xiaozhong Wang

   Article

   Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

   Insert

   RtcA shRNA-2
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   Mouse U6 promoter

   Available since

   June 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. AICSDP-15:ACTB-mEGFP

    Plasmid

    #87425

    Purpose

    Homology arms and linker-mEGFP sequence for N-terminus tagging of human ACTB

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    ACTB Homology Arms with mEGFP-linker (ACTB Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    March 21, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pCSC-NGN2-IRES-ISL1-T2A-LHX3

    Plasmid

    #221814

    Purpose

    Lentiviral vector expressing human NEUROG2, ISL1 and LHX3 for inducing pluripotent stem cells into cholinergic motor neurons.

    Depositor

    Baojin Ding

    Article

    Sepehrimanesh et al Am J Physiol Cell Physiol. 2020 Oct 1;31

    Inserts

    NEUROG2 (NEUROG2 Human)
    ISL1 (ISL1 Human)
    LHX3 (LHX3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCV-MYC-t2A-BCL2

    Plasmid

    #135306

    Purpose

    Overexpression of multiple human genes (BCL2 and MYC)

    Depositor

    Daniel Hodson

    Article

    Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

    Inserts

    MYC (MYC Human)
    BCL2 (BCL2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVET-HA-K-RasG12V-IRES-GFP

    Plasmid

    #107140

    Purpose

    A bicistronic lentiviral mammalian expression vector for HA-KRasG12V(4B) and GFP

    Depositor

    Aki Manninen

    Article

    Zhang et al Oncogene. 2017 Oct 12;36(41):5681-5694.

    Insert

    HA-tagged Kras4B(G12V) and GFP (KRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF-1alpha

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDmelOR-mRho.V5.mER.hOr56a

    Plasmid

    #126479

    Purpose

    Human codon-optimized D. melanogaster Orco (hOrco) and Or56a (hOr56a). Or56a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells

    Depositor

    Bill Hansson

    Article

    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

    Inserts

    Or56a (Or56a Fly)
    Orco (Orco Fly)
    

    Use

    Tags

    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…

    Expression

    Mammalian

    Mutation

    codon optimization for H. sapiens and codon optim…

    Promoter

    CMV

    Available since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-PGK-RAP1A-WT-puro

    Plasmid

    #156166

    Purpose

    Lentiviral vector for expression of RAP1A-WT, with puromycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP1A (RAP1A Human)
    

    Use

    Lentiviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    wild type form

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-PGK-RAP1A-E63-puro

    Plasmid

    #156167

    Purpose

    Lentiviral vector for expression of RAP1A-E63, with puromycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP1A (RAP1A Human)
    

    Use

    Lentiviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    changed Glutamine 63 to Glutamic acid

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-PGK-flag-RAP1B-V12-puro

    Plasmid

    #156168

    Purpose

    Lentiviral vector for expression of flag-RAP1B-V12, with puromycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP1B (RAP1B Bovine)
    

    Use

    Lentiviral

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    changed Glycine 12 to Valine

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-PGK-flag-RAP1B-N17-puro

    Plasmid

    #156169

    Purpose

    Lentiviral vector for expression of flag-RAP1B-N17, with puromycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP1B (RAP1B Bovine)
    

    Use

    Lentiviral

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    changed Serine 17 to Asparagine

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-PGK-RAP2A-WT-puro

    Plasmid

    #156170

    Purpose

    Lentiviral vector for expression of RAP2A-WT, with puromycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    wild type form

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-PGK-RAP2A-V12-puro

    Plasmid

    #156171

    Purpose

    Lentiviral vector for expression of RAP2A-V12, with puromycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    changed Glycine 12 to Valine

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge