We narrowed to 4,420 results for: DUR

1. PB09-TRE-beta-globin-miR-124-EF1a-GFP

   Plasmid

   #117318

   Purpose

   Overexpression of miR-124 in eurkaryotic cells, encoded in a human beta-globin intron

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   hsa-miR-124-1 (MIR124-1 Human)
   

   Use

   Transposon-mediated integration

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-inducible promoter

   Available since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIG-828_HA-GD2-28z_CAR_BATF_Retroviral

   Plasmid

   #207505

   Purpose

   This plasmid can be used to generate retrovirus.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   BATF, HA-GD2-28z_CAR (BATF Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXS-IRES-BLAST flag-CAD

   Plasmid

   #169818

   Purpose

   Expresses C-terminal flag-tagged CAD in mammalian cells

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   TCCC -> AGTC silent mutations at nt527-530 eli…

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXS-IRES-BLAST flag-CAD S1859A

   Plasmid

   #169821

   Purpose

   Expresses C-terminal flag-tagged CAD with mutation of reported S6K phosphorylation site

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   S1859A; TCCC -> AGTC silent mutations at nt527…

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLIK 3xHA-MKK6-EE neo

   Plasmid

   #47546

   Purpose

   Lentiviral expression vector for an inducible 3xHA-tagged constitutively active MKK6 mutant

   Depositor

   Kevin Janes

   Article

   Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-

   Insert

   MKK6-EE (MAP2K6 Human)
   

   Use

   Lentiviral

   Tags

   3XHA

   Expression

   Mammalian

   Mutation

   S207E, T211E

   Promoter

   Available since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. plenti-As-Cas12a-2xNLS

   Plasmid

   #155047

   Purpose

   Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged _Acidaminococcus sp. BV3L6 (As)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   AsCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS, Myc-tag and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-puro/RAF1-P261A

   Plasmid

   #131728

   Purpose

   Mammalian Expression of RAF1

   Depositor

   Jacques De Grève

   Article

   Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

   Insert

   RAF1 (RAF1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   P261A

   Promoter

   CMV

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLIK MEK5-DD-mRFP1 neo

   Plasmid

   #47548

   Purpose

   Lentiviral expression vector for an inducible mRFP1-tagged constitutively active MEK5 mutant

   Depositor

   Kevin Janes

   Article

   Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-

   Insert

   MEK5-DD-mRFP1 (MAP2K5 Human)
   

   Use

   Lentiviral

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   S311D, T315D

   Promoter

   Available since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

9. BPV00554 pET21GG2-His(6)-BECN(248-450)

   Plasmid

   #136685

   Purpose

   Expresses the Beclin-1 ECD/BARA domain (a.a. 248-450) with a N-terminal hexa-Histidine tag

   Depositor

   Colin Garvie

   Article

   Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

   Insert

   Beclin-1 ECD/BARA (BECN1 Human)
   

   Use

   Tags

   Hexa-Histadine

   Expression

   Bacterial

   Mutation

   DNA optimized for E. coli expression

   Promoter

   T7

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGem-sHyper:sAPX

    Plasmid

    #84738

    Purpose

    Simultaneous expresion of stromal sHyPer and stromal Ascorbate peroxidase (APX) in plant cells

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Inserts

    sAPX (SAPX Mustard Weed)
    sHyper (HyPer2 targeted to nucleus)
    

    Use

    Tags

    nuclear localisation signals from the coding sequ…

    Expression

    Plant

    Mutation

    Promoter

    pFMV/p35S

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGem-nHyPer:sAPX

    Plasmid

    #84735

    Purpose

    Simultaneous expresion of stromal HyPer2 and stromal Ascorbate peroxidase (APX) in plant cells

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Inserts

    sAPX (SAPX Mustard Weed)
    nHyper (HyPer2 targeted to nucleus)
    

    Use

    Tags

    nuclear localisation signals from the coding sequ…

    Expression

    Plant

    Mutation

    Promoter

    FMV/35S

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMD9_2xUGI_3xHA

    Plasmid

    #136652

    Purpose

    Expresses St1BE4max-LMD9 in mammalian cells along with its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Rat Alpha2 delta-1 pMT2

    Plasmid

    #58726

    Purpose

    expression of rat alpha2 delta-1 in mammalian cells

    Depositor

    Annette Dolphin

    Article

    Davies et al Proc Natl Acad Sci U S A. 2010 Jan 26;10

    Insert

    alpha2 delta-1 (Cacna2d1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Sept. 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-sgRNA-Cre-GpNLuc-sgmcGAS

    Plasmid

    #208385

    Purpose

    Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine cGAS gene.

    Depositor

    Gaorav Gupta

    Article

    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

    Insert

    sgcGAS (Cgas Mouse)
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP Cav2.2 HA D122A pcDNA3

    Plasmid

    #206120

    Purpose

    expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D122A

    Promoter

    CMV

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cav2.2 HA E1658A (EIVA) pcDNA3

    Plasmid

    #206114

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658A mutation in the P loop of Domain IV which reduces trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E1658A

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Cav2.1 EIVA (E1707A) pcDNA3

    Plasmid

    #206125

    Purpose

    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain IV to to abolish divalent cation binding

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E1707A

    Promoter

    CMV

    Available since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Cav2.1 HA EIA (E320A) pcDNA3

    Plasmid

    #206124

    Purpose

    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain I to abolish divalent cation binding

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E320A

    Promoter

    CMV

    Available since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLEX-FKBP-Kras-Blasticidin

    Plasmid

    #120714

    Purpose

    Expresses membrane-targeted recruiter protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    KRAS4B (KRAS Human)
    

    Use

    Lentiviral

    Tags

    FKBP and mCherry

    Expression

    Mammalian

    Mutation

    amino acids 158-188

    Promoter

    CMV

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. p1.1-mCherry

    Plasmid

    #162772

    Purpose

    Fluorescent reporter for CHO expression studies

    Depositor

    Ivan Vorobiev

    Article

    Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2

    Insert

    red fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    consensus Kozak sequence (GCCGCCATGG) added befor…

    Promoter

    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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