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pUAST attB BmOr2 (Y464A)

Plasmid

#73927

Purpose

UAS induced expression of Bombyx mori odorant receptor 2 (OR2) cDNA with 464 amino acid change of Tyrosine to Alanine

Depositor

Leslie Vosshall

Article

Nakagawa et al PLoS One. 2012;7(3):e32372. doi: 10.1371

Insert

BmOr2 (Y464A)

Use

Tags

Expression

Insect

Mutation

changed 464 Tyrosine to Alanine

Promoter

hsp70

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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RP185 pUAST EGFP:Or47b

Plasmid

#59525

Purpose

N-terminal fusion of GFP to Drosophila melanogaster Or47b

Depositor

Leslie Vosshall

Article

Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

Insert

EGFP:Or47b (Or47b Fly)

Use

Tags

EGFP

Expression

Insect

Mutation

Promoter

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
Syn3D71

Plasmid

#14680

Depositor

Thomas Weimbs

Article

Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

Insert

Syntaxin3 (STX3 Human)

Use

Tags

2xMyc-His

Expression

Mammalian

Mutation

Insert is cloned into a modified vector; aa 1-71 …

Promoter

Available since

April 20, 2007

Availability

Academic Institutions and Nonprofits only
Syn3D96

Plasmid

#14681

Depositor

Thomas Weimbs

Article

Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

Insert

Syntaxin3 (STX3 Human)

Use

Tags

2xMyc-His

Expression

Mammalian

Mutation

Insert is cloned into a modified vector; aa 1-96 …

Promoter

Available since

April 20, 2007

Availability

Academic Institutions and Nonprofits only
Syn3D119

Plasmid

#14682

Depositor

Thomas Weimbs

Article

Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

Insert

Syntaxin3 (STX3 Human)

Use

Tags

2xMyc-His

Expression

Mammalian

Mutation

Insert is cloned into a modified vector; aa 1-119…

Promoter

Available since

April 20, 2007

Availability

Academic Institutions and Nonprofits only
Syn3F35A

Plasmid

#13402

Depositor

Thomas Weimbs

Article

Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

Insert

Syntaxin3 (STX3 Human)

Use

Tags

Myc-His

Expression

Mammalian

Mutation

Insert is cloned into a modified vector. F35 of s…

Promoter

Available since

Nov. 9, 2006

Availability

Academic Institutions and Nonprofits only
Syn3F36A

Plasmid

#13403

Depositor

Thomas Weimbs

Article

Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

Insert

Syntaxin3 (STX3 Human)

Use

Tags

Myc-His

Expression

Mammalian

Mutation

Insert is cloned into a modified vector. F36 of s…

Promoter

Available since

Nov. 9, 2006

Availability

Academic Institutions and Nonprofits only
Cdk12_intron3_sg2_pX330

Plasmid

#127174

Purpose

sgRNA that cuts within intron 3 of mouse Cdk12 genomic locus in pX330 backbone

Depositor

Phil Sharp

Article

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

Mouse Cdk12 Intron 3 sgRNA

Use

CRISPR and Mouse Targeting

Tags

Expression

Mutation

Promoter

U6 Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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Cdk12_intron4_sg1_pX458

Plasmid

#127175

Purpose

sgRNA that cuts within intron 4 of mouse CDK12 genomic locus in pX458 backbone

Depositor

Phil Sharp

Article

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

Mouse Cdk12 Intron 4 sgRNA

Use

CRISPR and Mouse Targeting

Tags

Expression

Mutation

Promoter

U6 Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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EF1a_mCherry_P2A_Hygro

Plasmid

#135003

Purpose

Lentiviral vector to express mCherry or to clone and express gene of interest, under control of EF1a promoter. Barcodes compatible with detection in single cell RNA-seq may be inserted at HpaI site.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pZS157 CRISPEY RT/Cas9

Plasmid

#114454

Purpose

Yeast Integration Plasmid for galactose inducible Ec86-RT and SpCas9 expression

Depositor

Hunter Fraser

Article

Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

Insert

SpCas9, Ec86-RT

Use

CRISPR

Tags

SV40-NLS

Expression

Yeast

Mutation

S. cerevisiae codon optimized

Promoter

GAL1-GAL10

Available since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFR-miRFP670nano3

Plasmid

#184673

Purpose

EGF receptor (EGFR) labelled with near-infrared fluorescent protein miRFP670nano3

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

EGFR-miRFP670nano3

Use

Tags

miRFP670nano3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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TDP-43 mRuby

Plasmid

#216225

Purpose

TDP-43 with a C-terminal mRuby tag for expression in mammalian cells.

Depositor

Jennifer Lee

Article

Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do

Insert

TDP-43 (TARDBP Human)

Use

Tags

mRuby

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pvimentin-emiRFP670

Plasmid

#136572

Purpose

Vimentin labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

vimentin-emiRFP670

Use

Tags

emiRFP670

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pLAMP1-emiRFP670

Plasmid

#136570

Purpose

LAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

LAMP1-emiRFP670

Use

Tags

emiRFP670

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFR-PATagRFP

Plasmid

#31950

Depositor

Vladislav Verkhusha

Article

Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

Insert

PATagRFP

Use

Tags

EGF receptor (EGFR)

Expression

Mammalian

Mutation

R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

Promoter

CMV

Available since

Oct. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pH2B-emiRFP670

Plasmid

#136571

Purpose

Histone 2B labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

H2B-emiRFP670

Use

Tags

emiRFP670

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA Flag Yap1

Plasmid

#18881

Depositor

Yosef Shaul

Article

Levy et al Mol Cell. 2008 Feb 15. 29(3):350-61.

Insert

Yap1 (YAP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 14, 2008

Availability

Academic Institutions and Nonprofits only

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piRFP

Plasmid

#31857

Purpose

a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

Depositor

Vladislav Verkhusha

Article

Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

Has Service

Cloning Grade DNA

Insert

iRFP

Use

Tags

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…

Promoter

CMV

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pvimentin-emiRFP703

Plasmid

#136566

Purpose

Vimentin labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

vimentin-emiRFP703

Use

Tags

emiRFP703

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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