We narrowed to 11,272 results for: VARS

1. pZH514

   Plasmid

   #102667

   Purpose

   Constitutive expression of GFPmut2; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

   Depositor

   Zach Hensel

   Article

   Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

   Insert

   GFPmut2
   

   Expression

   Bacterial

   Available Since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX165-Sniper-Cas9

   Plasmid

   #140560

   Purpose

   Expresses Sniper Cas9 in mammalian cells

   Depositor

   Feng Zhang

   Article

   Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

   Insert

   Cas9
   

   Mutation

   F539S M763I K890N

   Available Since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a_speG_Cterm_His

   Plasmid

   #220483

   Purpose

   heterologous expression for protein purification

   Depositor

   Emily Balskus

   Article

   Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

   Insert

   speG
   

   Tags

   6xHs

   Expression

   Bacterial

   Mutation

   none

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZH516

   Plasmid

   #102669

   Purpose

   Constitutive expression of GFPmut2; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

   Depositor

   Zach Hensel

   Article

   Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

   Insert

   GFPmut2
   

   Expression

   Bacterial

   Available Since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKSB-610 U6-sgRNA2 GGAA AGAG

   Plasmid

   #173672

   Purpose

   To clone and express a gRNA in Anopheles

   Depositor

   Eric Marois

   Article

   Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX165-Cas9-HF1

   Plasmid

   #140565

   Purpose

   Expresses Cas9-HF1 in mammalian cells

   Depositor

   Feng Zhang

   Article

   Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

   Insert

   Cas9
   

   Mutation

   N497A R661A Q695A Q926A

   Available Since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a_PA4114_Cterm_His

   Plasmid

   #220484

   Purpose

   heterologous expression of PA4114 for protein purification

   Depositor

   Emily Balskus

   Article

   Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

   Insert

   PA4114
   

   Tags

   6xHs

   Expression

   Bacterial

   Mutation

   none

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBAD/HisD-TagRFP675

   Plasmid

   #44274

   Depositor

   Vladislav Verkhusha

   Article

   Piatkevich et al Sci Rep. 2013;3:1847. doi: 10.1038/srep0

   Insert

   TagRFP675
   

   Expression

   Bacterial

   Mutation

   M41Q/F80W/S143N/L147M/S158N/D159Y/N173S

   Promoter

   pBAD

   Available Since

   May 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJY3919

   Plasmid

   #113332

   Purpose

   Hermes transposable plasmid; TIR-flanked LEU2, recoded transposase driven by TEF1 promoter

   Depositor

   Suzanne Sandmeyer

   Article

   Patterson et al Metab Eng. 2018 May 22;48:184-196. doi:

   Inserts

   beta-isopropyl malate dehydrogenase
   codon-optimized Hermes transposase
   

   Expression

   Yeast

   Available Since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-HA-MAVS-M142A(KaganF61)

    Plasmid

    #51999

    Purpose

    Expresses the human MAVS CDS containing the point mutation M142A and an N-terminal HA tag "strong translational context"

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    MAVS
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Met 142 to Ala

    Available Since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pCMV5 MYC-Smurf2 (FF29/30A) WT

    Plasmid

    #24604

    Depositor

    Jeff Wrana

    Article

    Ogunjimi et al J Biol Chem. 2010 Feb 26. 285(9):6308-15

    Insert

    Smurf2 (SMURF2 Human)
    

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    F29A, F30A

    Available Since

    April 30, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. VZV ORF47

    Plasmid

    #26698

    Depositor

    Robert Kalejta

    Article

    Kuny et al PLoS Pathog. 2010 . 6(9):.

    Insert

    ORF47 (ORF47 Varicella Zoster Virus (HHV-3))
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    A 3B3 epitope tag (DQRQYGDVFKGD) from the VZV gB …

    Available Since

    Nov. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pLX_313-EGFP

    Plasmid

    #158580

    Purpose

    Lentiviral expression of EGFP

    Depositor

    John Doench, David Root

    Article

    Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

    Insert

    EGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRDA_450

    Plasmid

    #179094

    Purpose

    nuclease Cas expression

    Depositor

    John Doench, David Root

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    SpRY
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET15b I-SceI (Ctermhis -MH)

    Plasmid

    #195613

    Purpose

    Expresses the wild-type I-SceI homing endonuclease from the T7 promoter

    Depositor

    Frederick Gimble

    Article

    Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

    Insert

    I-SceI
    

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-R4-uAb

    Plasmid

    #101801

    Purpose

    Bacterial cell expression of the beta-galactosidase-specific ubiquibody, R4-uAb

    Depositor

    Matthew DeLisa

    Article

    Portnoff et al J Biol Chem. 2014 Mar 14;289(11):7844-55

    Insert

    R4-uAb
    

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. p1.2-Hygro

    Plasmid

    #162741

    Purpose

    Protein expression in CHO cells

    Depositor

    Ivan Vorobiev

    Article

    Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CHO Elongation factor 1A

    Available Since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. p1.2-Neo

    Plasmid

    #162739

    Purpose

    Protein expression in CHO cells

    Depositor

    Ivan Vorobiev

    Article

    Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CHO Elongation factor 1A

    Available Since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330A_FokI-1x2

    Plasmid

    #63602

    Purpose

    Expresses FokI-dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    FokI-dCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQP-2473

    Plasmid

    #138976

    Purpose

    Nucleus-to-cytoplasm relocalization of genomically expressed GFP-fusion using NIR light controlled intrabody.

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

    Insert

    NcoI-BphP1-NES-T2A- iB(GFP)-NES-mCherry-QPAS1- NLS-NotI
    

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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