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pAc5.1B-lambdaN-HA-DmNot3_A

Plasmid

#145895

Purpose

Insect Expression of DmNot3

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmNot3 (Not3 Fly)

Expression

Insect

Mutation

two silent mutations compared to the sequence giv…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-F2V2_A

Plasmid

#145896

Purpose

Insect Expression of DmAGO1-F2V2

Depositor

Elisa Izaurralde

Article

Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

Insert

DmAGO1-F2V2 (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations compared to…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-F594V_A

Plasmid

#145897

Purpose

Insect Expression of DmAGO1-F594V

Depositor

Elisa Izaurralde

Article

Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

Insert

DmAGO1-F594V (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations compared to…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-siRNAres_A

Plasmid

#145899

Purpose

Insect Expression of DmAGO1-siRNAres

Depositor

Elisa Izaurralde

Article

Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

Insert

DmAGO1-siRNAres (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations compared to…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pnYC-NvHN-DmXRN1_1323-1355_N

Plasmid

#147052

Purpose

Bacterial Expression of DmXRN1_1323-1355

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmXRN1_1323-1355 (pcm Fly)

Expression

Bacterial

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pGMC00008

Plasmid

#166724

Purpose

sgRNA cloning backbone

Depositor

Raj Chari

Article

Choi et al Nat Commun. 2020 Jun 1;11(1):2718. doi:

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-F629V_A

Plasmid

#145898

Purpose

Insect Expression of DmAGO1-F629V

Depositor

Elisa Izaurralde

Article

Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

Insert

DmAGO1-F629V (AGO1 Fly)

Expression

Insect

Mutation

V315A, F629V, H913R - variation with 2 non silent…

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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OA-1045B

Plasmid

#125004

Purpose

Expresses gRNAs targeting hid, eve, and hedgehog

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

eve gRNA (eve Fly)

Expression

Insect

Promoter

U6:3

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1045D

Plasmid

#125006

Purpose

Expresses gRNAs targeting hedgehog and wingless

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

wg gRNA (wg Fly)

Expression

Insect

Promoter

u6:3

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1045E

Plasmid

#125007

Purpose

Expresses tRNA-flanked gRNAs targeting hid, eve, and hedgehog

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

eve gRNA (eve Fly)

Expression

Insect

Promoter

U6:3

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1045A

Plasmid

#125003

Purpose

Expresses gRNAs targeting hid and eve

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

eve gRNA (eve Fly)

Expression

Insect

Promoter

U6:3

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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Drosophila Nedd2-like caspase (DRONC)

Plasmid

#157778

Purpose

E. coli expression vector pET23 with C-term 6xHis tag

Depositor

Guy Salvesen

Article

Snipas et al Cell Death Differ. 2008 May;15(5):938-45

Insert

Drosophila Nedd2-like caspase (DRONC) (Dronc Fly)

Tags

6x HIS

Expression

Bacterial

Promoter

T7

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pET28P_dCrk

Plasmid

#131145

Purpose

Expresses 6XHis::dCrk (based on crk isoform RA).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

Crk (Crk Fly)

Tags

6XHis

Expression

Bacterial

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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gRNAs[bTub+Tra].1046D

Plasmid

#112693

Purpose

express two gRNA targeting bTub & Tra under dU6-3 promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

U6.3-gRNA[bTub] & U6.3-gRNA[Tra] (tra Fly)

Use

CRISPR

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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gRNA[bTub+DsxF].1046A

Plasmid

#112694

Purpose

express two gRNA targeting bTub & DsxF under dU6-3 promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

U6.3-gRNA[bTub] & U6.3-gRNA[DsxF] (dsx Fly)

Use

CRISPR

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pOU2

Plasmid

#41754

Depositor

Jeff Gelles

Article

Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

kinesin heavy chain (Khc Fly, E. coli)

Tags

His6 and bccp

Expression

Bacterial

Mutation

residues 1-401; C45S C338S C451S

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF3H09

Plasmid

#71003

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

SNF4Agamma (SNF4Agamma Fly)

Use

Gateway entry vector

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF3C07

Plasmid

#70985

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

14-3-3zeta (14-3-3zeta Fly)

Use

Gateway entry vector

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2B09

Plasmid

#70918

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

gro (gro Fly)

Use

Gateway entry vector

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF3C03

Plasmid

#70959

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Taf4 (Taf4 Fly)

Use

Gateway entry vector

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only