Addgene

We narrowed to 6,341 results for: Mag

Showing: 3751 - 3780 of 6341 results

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pAAV-Syn-Ace-mNeon2

Plasmid

#195518

Purpose

Green fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

Ace-mNeon2

Use

AAV

Tags

Expression

Mammalian

Mutation

Ace-mNeon 81S; SY linker

Promoter

Synapsin

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_eCBmut-IRES-mCherry-CAAX

Plasmid

#164612

Purpose

Express the endocannabinoid non-responsive sensor GRAB_eCB2.0 in mammalian cells

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid non-responsive sensor GRAB_eCBmut

Use

Tags

Expression

Mammalian

Mutation

S383T, F177A

Promoter

CMV

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-Tubulin-C-18

Plasmid

#55148

Purpose

Localization: Microtubules, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Fiolka et al Proc Natl Acad Sci U S A. 2012 Apr 3;109

Insert

Tubulin (TUBA1B Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
GFP-Ub KO

Plasmid

#11934

Depositor

Nico Dantuma

Article

Bergink et al Genes Dev. 2006 May 15. 20(10):1343-52.

Insert

Ubiquitin KO (RBX1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

All 7 lysines of ubiquitin mutated to arginines.

Promoter

Available since

Aug. 7, 2006

Availability

Academic Institutions and Nonprofits only

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pFast-His6-hRad17

Plasmid

#222465

Purpose

Expresses hRad17 in insect cells

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

Insert

hRad17 (RAD17 Human)

Use

Tags

His

Expression

Insect

Mutation

Promoter

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pME-Sun1-2xsfGFP-6xMYC-pA (JDW 681)

Plasmid

#224489

Purpose

Gateway compatible middle entry clone containing Sun1 reporter fusion to 2 copies of sfGFP and 6 MYC tags (Nuclear envelope reporter and affinity tag)

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

Sun1-2xsfGFP-6xMYC-pA

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-synapsin-WHaloCaMP1a

Plasmid

#205308

Purpose

Production of AAV particles encoding WHaloCaMP1a from the synapsin promoter

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1a

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pME-DreERT2 (JDW 1344)

Plasmid

#224523

Purpose

A Gateway compatible middle entry clone containing Dre recombinase fused to mutant ERT2 for tamoxifen-inducible recombinase activity

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

DreERT2

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-dLight1.3b

Plasmid

#111056

Purpose

Expresses dLight1.3b in mammalian cells

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Insert

dLight1.3b

Use

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Puro-CAG-mNG2(1-10)

Plasmid

#206042

Purpose

Repair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.

Depositor

Alisa Piekny

Article

Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7

Insert

AAVS1 homology arms with SA-P2A-Puro marker and CAG-mNG2(1-10) expression cassette

Use

CRISPR and Synthetic Biology; Donor template

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-G-Flamp1-mut

Plasmid

#188568

Purpose

control probe of G-Flamp1, insensitive to cAMP

Depositor

Jun Chu

Article

Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

Insert

G-Flamp1-mut

Use

Tags

His tag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Pyronic/pcDNA3.1(-)

Plasmid

#51308

Purpose

Pyronic, FRET sensor that allow quantitative measurement of pyruvate transport, pyruvate production and mitochondrial pyruvate consumption in intact individual cells at high temporal resolution.

Depositor

Luis Felipe Barros

Article

San Martin et al PLoS One. 2014 Jan 21;9(1):e85780. doi:

Insert

Pyronic

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.GCaMP6s.WPRE.SV40

Plasmid

#100844

Purpose

AAV expression of ultrasensitive protein calcium sensor from CAG promoter

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Has Service

AAV1, AAV5, and AAV9

Insert

GCaMP6s

Use

AAV

Tags

Expression

Mammalian

Mutation

GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

Promoter

CAG

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-mGLIS1

Plasmid

#33273

Purpose

Retroviral expression of mouse Glis1

Depositor

Shinya Yamanaka

Article

Maekawa et al Nature. 2011 . 474(7350):225-9.

Insert

GLIS1, Gateway casette A (Glis1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLEX-RiboL1-jGCaMP8m

Plasmid

#192617

Purpose

AAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

FLEX-RiboL1-jGCaMP8m

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-tagRFP-FLAG-cGAS

Plasmid

#86676

Purpose

Expresses catalytically-active tagRFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

Depositor

Nicolas Manel

Article

Raab et al Science. 2016 Apr 15;352(6283):359-62. d

Insert

tagRFP-FLAG-cGAS

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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mVenus-IQ6 R6A

Plasmid

#198202

Purpose

FRET acceptor: Mutated IQ6 domain of Moysin Va tagged with N-terminal mVenus (lower binding affinity)

Depositor

Christian Wahl-Schott

Article

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Insert

MYO5A IQ domain (Myo5a Mouse)

Use

Tags

mVenus

Expression

Mammalian

Mutation

R6A

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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Tg(mpeg1:LifeAct-mRuby)

Plasmid

#166977

Purpose

labels F-actin in macrophages

Depositor

Anna Huttenlocher

Article

Barros-Becker et al J Cell Sci. 2017 Nov 15;130(22):3801-380

Inserts

mpeg1 (mpeg1.1 Zebrafish)
LifeAct (actb1 Frog)

Use

Tags

Lifeact-mRuby and mRuby

Expression

Mutation

Promoter

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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VN-beta Synuclein

Plasmid

#89483

Purpose

expresses human beta Synuclein fused to the N-terminal part of Venus

Depositor

Tiago Outeiro

Article

Tenreiro et al Hum Mol Genet. 2016 Jan 15;25(2):275-90.

Insert

SNCB (SNCB Human)

Use

Tags

N-terminal part of Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pLX307-VHL(Y98H)-3F-BirA

Plasmid

#220148

Purpose

Expresses VHL(Y98H)-BirA as a control for VHL substrate identification by E-STUB

Depositor

William Sellers

Article

Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

Insert

von Hippel-Lindau tumor suppressor (VHL Human)

Use

Lentiviral

Tags

3F-BirA

Expression

Mammalian

Mutation

changed tyrosine 98 to histidine; codon optimized

Promoter

EF-1a

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-dLight1.2

Plasmid

#111054

Purpose

Expresses dLight1.2 in mammalian cells

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Insert

dLight1.2

Use

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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tetO-SMAD6

Plasmid

#170700

Purpose

doxycycline-inducible overexpression of SMAD6

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

SMAD6 (SMAD6 Human)

Use

Lentiviral; Doxycycline inducible

Tags

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet-ON

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-RdLight1

Plasmid

#125708

Purpose

Expresses the red genetically encoded dopamine sensor RdLight1 in mammalian neurons.

Depositor

Lin Tian

Article

Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

Has Service

AAV9

Insert

RdLight1

Use

AAV

Tags

Flag tag

Expression

Mutation

Promoter

hSynapsin1

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-5U-Venus-PSD-95-3U

Plasmid

#102949

Purpose

Expresses Venus-PSD-95 in mammalian cells

Depositor

Gary Bassell

Article

Ifrim et al J Neurosci. 2015 May 6;35(18):7116-30. d

Insert

PSD-95 (Dlg4 Mouse)

Use

Tags

Venus Fluorescent protein

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-RdLight1

Plasmid

#125707

Purpose

Expresses the red genetically encoded dopamine sensor RdLight1 in mammalian cells.

Depositor

Lin Tian

Article

Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

Has Service

AAV9

Insert

dLight1.3b

Use

AAV

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pME Actin-Vhh sfGFP (JDW 1215)

Plasmid

#224495

Purpose

Gateway compatible middle entry clone with Actin nanobody fused to sfGFP (GFP actin reporter)

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

Actin-Vhh-sfGFP

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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tetO-MYOCD

Plasmid

#170696

Purpose

doxycycline-inducible overexpression of MYOCD

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

MYOCD (MYOCD Human)

Use

Lentiviral; Doxycycline inducible

Tags

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet-ON

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pFLAG AXL

Plasmid

#105933

Purpose

Mammalian expression of Flag-tagged AXL

Depositor

Rosa Marina Melillo

Article

Krishnamoorthy et al J Biol Chem. 2013 Jun 14;288(24):17481-9

Insert

AXL (AXL Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-Voltron

Plasmid

#119033

Purpose

Express Voltron in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Insert

Voltron

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry

Plasmid

#124434

Purpose

Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in Drosophila

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipGFP(Casp3 cleavage seq) T2A mCherry

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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