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Plasmid#231594PurposeExpress high ribosome loading circular RNA translation reporter with 2xALFATag-10xSunTag under H1 promoterDepositorInsertKozak sequence-Tornado-10xSunTag-2xALFATag
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
TS module
Plasmid#26021DepositorInsertTension Sensor module
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
6xHis(TEV)-Drp1(K38A)-StrepII
Plasmid#174423PurposeExpresses human Drp1 Isoform 3 with K38A mutation in bacteriaDepositorInsertDrp1
UseTags6xHis(TEV) and StrepIIExpressionBacterialMutationK38APromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
P[acman]-Alpha2
Plasmid#165859PurposeAlpha2 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
"AND-gate" receiver plasmid
Plasmid#127676PurposeEncodes the transcriptional activator LuxR and GFP. LuxR has an pLlacO-1 promoter and the GFP has a pLux promoter.DepositorInsertLuxR, GFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLlacO-1 for LuxR, pLux for GFPAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
HH-gRNA-HDV-Shuttle-Vector
Plasmid#169098PurposeBackbone plasmid to generate HH-gRNA-HDV segmentDepositorInsertHH-gRNAbackbone-HDV
UseTagsExpressionMammalianMutationPromoterNAAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTW199b2
Plasmid#136928PurposeDirected evolution of SpCas9DepositorInsertgIII
UseCRISPRTagsExpressionBacterialMutationnonePromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWB402SC
Plasmid#224552PurposeEmpty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes C-terminal Twin-Strep-HA tags. Duplicated 35S promoter and 35S terminator.DepositorTypeEmpty backboneUseTagsTwin-Strep-HAExpressionPlantMutationPromoter2 X 35SAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB402SN
Plasmid#224551PurposeEmpty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes an N-terminal HA-Twin-Strep tag. Duplicated 35S promoter and 35S terminator.DepositorTypeEmpty backboneUseTagsHA-Twin-StrepExpressionPlantMutationPromoter2 X 35SAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-CamKIIa-ChR2-EGFP
Plasmid#114216PurposeExpresses channelrhodopsin-EGFP fusion in CamKIIa+ neuronsDepositorInsertChannelrhodopsin 2
UseAAV and Mouse TargetingTagsEGFPExpressionMammalianMutationPromoterCamKIIa 1.3 kbAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 S6K2 WT
Plasmid#17729DepositorInsertS6K2 (RPS6KB2 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA3-TSC1
Plasmid#19911DepositorInsertTsc1 (Tuberous sclerosis 1) (TSC1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCS2-epNTR
Plasmid#62213PurposeepNTR (zebrafish optimized nitroreductase, T41L, N71S, F124W) in pCS2DepositorInsertepNTR
UseMrna synthesisTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TetON-CD4
Plasmid#217537PurposeAAV vector to drive the expression of Cre- and doxycycline-dependent expression of HA-epitope tagged CD4 receptorDepositorArticleInsertCD4 receptor (CD4 Human)
UseAAVTagsHA epitopeExpressionMammalianMutationPromoterTetOperon (TetO)Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCGN-S2P-WT
Plasmid#32957PurposeFor mammalian expression of human S2P with an HA tagDepositorInsertS2P (MBTPS2 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTW170b2
Plasmid#136929PurposeDirected evolution of SpCas9DepositorInsertgVI
UseCRISPRTagsExpressionBacterialMutationnonePromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG>nls-Cas9-nls-2A-Citrine-EcoRI/NotI
Plasmid#169097PurposeCas9 and Citrine expression in transfected cellsDepositorInsertCas9-2A-Citrine
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTW195b63
Plasmid#136931PurposeDirected evolution of SpCas11DepositorInsertgIII(N)-npuN
UseCRISPRTagsExpressionBacterialMutationnonePromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
hRad51(K133R)–Cas9(D10A)
Plasmid#125568PurposeExpresses the Rad51(K133R)–Cas9(D10A) fusion construct in mammalian cells under CMV promoterDepositorInsertRad51(K133R)–Cas9(D10A) (RAD51 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-12xLexAOp-mCherry-MiniWhite
Plasmid#165917PurposeBlasticidin resistant 12xLexAOp mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable LexAOp Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIRES RED/SP M87C448Y
Plasmid#89324PurposeExpression of RFP and M87C448Y spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-460 deletedPromoterCMVAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
paGFP-HRas
Plasmid#18693DepositorInsertH-Ras (HRAS Human)
UseTagsphotoactivatable GFPExpressionMammalianMutationA206K mutation in paGFPPromoterAvailable sinceJuly 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
T7RNAP Cyslight V174C
Plasmid#205078PurposeT7RNAP protein expressionDepositorInsertT7RNAP Cyslight V174C (T7p07 Bacteriophage T7)
UseTagsExpressionBacterialMutationC125S, C347S, C510S, C515S, C530S, C723S V174CPromoterAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
NHA-hnRNPK
Plasmid#120389PurposeExpression of HA tagged hnRNPK-lambdaN fusionDepositorInserthnRNPK (HNRNPK Human)
UseTagsHA and LambdaNExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 S6K2 KR
Plasmid#17730DepositorInsertS6K2 (RPS6KB2 Human)
UseTagsHAExpressionMammalianMutationK99R: kinase dead mutantPromoterAvailable sinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCGN-ELK-1 S383A
Plasmid#27160PurposeFor mammalian expression of human Elk-1 with an HA tag and a mutation in the MAPK phosphorylation site of the transcriptional activation domainDepositorInsertELK1 (ELK1 Human)
UseTagsHAExpressionMammalianMutationTAD mutant: S383APromoterAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
LZRS-RasG12V
Plasmid#21203DepositorInsertRas (HRAS Human)
UseRetroviralTagsExpressionMutationG12VPromoterAvailable sinceJuly 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCruzHA SIRT1 G261A
Plasmid#10963DepositorInsertSIRT1 (Sirt1 Mouse)
UseTagsHAExpressionMammalianMutationG261APromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-miR-29b-1~29a
Plasmid#51376DepositorInsertpri-miR-29b-1~29a (MIR29B1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCGN-ELK-1 Ets mut
Plasmid#27157PurposeFor mammalian expression of human Elk-1 with an HA tag and a mutation in the Ets DNA binding domainDepositorInsertELK1 (ELK1 Human)
UseTagsHAExpressionMammalianMutationR62E/R65E/Y66DPromoterAvailable sinceDec. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTW194b63
Plasmid#136930PurposeDirected evolution of SpCas10DepositorInsertnpuC-gIII(C)
UseCRISPRTagsExpressionBacterialMutationnonePromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pINTO-N-FH
Plasmid#65924PurposeInducible expression of proteins fused to an N-terminal Flag-HA tag (FH)DepositorTypeEmpty backboneUseTagsFLAG-HAExpressionMammalianMutationPromoterCMV/TetO2 (T-REx)Available sinceJuly 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xUAS-sfGFP-GMRWhite
Plasmid#165907PurposeBlasticidin resistant 5xUAS sfGFP GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable UAS Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag/WT M87
Plasmid#89322Purposeexpression of WT M87 spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-460 bp deletedPromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO {ChETA}on-WPRE
Plasmid#111386PurposeAAV expression of ChETA (hChR2 with E123T/H134R mutations) fused to HA tag driven by hSynapsin promoter in Cre-ON manner for optogenetic activationDepositorInsertChETA
UseAAVTagsHAExpressionMammalianMutationhumanized ChR2, E123T/H134RPromoterhSynapsinAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
P[acman]-Omega2
Plasmid#165861PurposeOmega2 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-NES-MBP-cATOM(Ras)
Plasmid#209711PurposeMammalian expression of c-ATOM biosensor for HRas with nuclear export signalDepositorInsertNES-MBP-cATOM(Ras)
UseTagsNES-MBPExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag/M1C448Y
Plasmid#89319Purposeexpression of M1 C448Y spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-194 bp of 5'UTR deleted, C448YPromoterCMVAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBabe-vHras-zeo
Plasmid#17756DepositorInsertvHras (genomic0 (HRAS Human)
UseRetroviralTagsExpressionMammalianMutationV12DPromoterAvailable sinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xUAS-sfGFP-MiniWhite
Plasmid#165910PurposeBlasticidin resistant 5xUAS sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable UAS Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only