We narrowed to 10,133 results for: gnas
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Plasmid#217694PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRF1 (ADGRF1 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-566Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRD2 CTF-FLAG
Plasmid#217688PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRD2 (ADGRD2 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-636Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRD1 CTF-FLAG
Plasmid#217687PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRD1 (ADGRD1 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-544Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRF5 CTF-FLAG
Plasmid#217698PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRF5 (ADGRF5 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-990Available SinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRG1 CTF-FLAG
Plasmid#217699PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRG1 (ADGRG1 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-382Available SinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1751 - pcDNA3.1 CMV-IE V5-ER-LBD
Plasmid#201818PurposeA plasmid expressing the ligand-binding domain of estrogen receptor with a V5 epitope tag from a CMV promoterDepositorInsertV5-ER-LBD
ExpressionMammalianPromoterCMV-IEAvailable SinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV1-DIO-eGFP-pvRPL10a
Plasmid#202542PurposeCr-dependent (DIO) expression of eGFP-tagged ribosomal subunit that uses the prairie vole RPL10a gene sequence under the human synapsin promoter.DepositorInsertRPL10a subunit of the ribosome
UseAAV and Cre/LoxTagseGFPExpressionMammalianMutationoptimized for prairie vole DNA sequence and optim…Promoterhuman synapsinAvailable SinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2trLATm2allL+A
Plasmid#203750PurposeEncodes the transmembrane domain from human LAT with mEos3.2 fused on the C terminus. Residues within the transmembrane domain mutated to L and A to increase the TMD interfacial surface area with the membrane. Used as a fluorescent plasma membrane probe.DepositorAvailable SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-δ
Plasmid#197259PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-3 δ fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)DepositorInsertAP-3 δ (AP3D1 Human)
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationVal 549 Ile substitution; silent substitutions in…PromoterADH1Available SinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-ε
Plasmid#197261PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-4 ε fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)DepositorInsertAP-4 ε (AP4E1 Human)
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationsilent substitutions in codons 905, 1129 and 1137PromoterADH1Available SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_SP-HTR2A-NTEV-TCS-GV-2xHA
Plasmid#194367PurposeSplit TEV assaysDepositorInsertSP-HTR2A-NTEV-TCS-GV-2xHA (HTR2A Human, Synthetic)
Tags2xHAExpressionMammalianPromoterCMVAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_HTR2A-V2R-NTEV-TCS-GV-2xHA
Plasmid#194368PurposeSplit TEV assaysDepositorInsertHTR2A-V2R-NTEV-TCS-GV-2xHA (HTR2A Human, Synthetic)
Tags2xHAExpressionMammalianPromoterCMVAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_SP-HTR2A-V2R-NTEV-TCS-GV-2xHA
Plasmid#194369PurposeSplit TEV assaysDepositorInsertSP-HTR2A-V2R-NTEV-TCS-GV-2xHA (HTR2A Human, Synthetic)
Tags2xHAExpressionMammalianPromoterCMVAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_AVPR2-V2R-NTEV-TCS-GV-2xHA
Plasmid#194372PurposeSplit TEV assaysDepositorInsertAVPR2-V2R-NTEV-TCS-GV-2xHA (AVPR2 Human, Synthetic)
Tags2xHAExpressionMammalianPromoterCMVAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_SP-AVPR2-V2R-NTEV-TCS-GV-2xHA
Plasmid#194373PurposeSplit TEV assaysDepositorInsertSP-AVPR2-V2R-NTEV-TCS-GV-2xHA (AVPR2 Human, Synthetic)
Tags2xHAExpressionMammalianPromoterCMVAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_SP-GCGR-NTEV-TCS-GV-2xHA
Plasmid#194375PurposeSplit TEV assaysDepositorAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_GCGR-V2R-NTEV-TCS-GV-2xHA
Plasmid#194376PurposeSplit TEV assaysDepositorInsertGCGR-V2R-NTEV-TCS-GV-2xHA (GCGR Human, Synthetic)
Tags2xHAExpressionMammalianPromoterCMVAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_SP-DRD1-V2R-NTEV-TCS-GV-2xHA
Plasmid#194361PurposeSplit TEV assaysDepositorInsertSP-DRD1-V2R-NTEV-TCS-GV-2xHA (DRD1 Human, Synthetic)
Tags2xHAExpressionMammalianPromoterCMVAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_SP-DRD2-NTEV-TCS-GV-2xHA
Plasmid#194363PurposeSplit TEV assaysDepositorAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_DRD2-V2R-NTEV-TCS-GV-2xHA
Plasmid#194364PurposeSplit TEV assaysDepositorInsertDRD2-V2R-NTEV-TCS-GV-2xHA (DRD2 Human, Synthetic)
Tags2xHAExpressionMammalianPromoterCMVAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_SP-DRD2-V2R-NTEV-TCS-GV-2xHA
Plasmid#194365PurposeSplit TEV assaysDepositorInsertSP-DRD2-V2R-NTEV-TCS-GV-2xHA (DRD2 Human, Synthetic)
Tags2xHAExpressionMammalianPromoterCMVAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_mGFP-FGFR3-Y373C
Plasmid#191754PurposeExpression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-Y373C mutation (associated with TD1) (target mutation: c.1118A>G ; silent mutation added on purpose: c.1110C>A)DepositorInsertmGFP-FGFR3IIIc
TagsmGFPExpressionMammalianMutationc.1118A>G, p.Y373C + c.1110C>A (silent muta…Available SinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKTop::sptse5-CT (1169-1229)
Plasmid#192948PurposeFor membrane insertion study; expresses Tse5-CT (1169-1229)/PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminusDepositorInsertsptse5-CT (1169-1229)
TagsPelB signal peptideExpressionBacterialMutationencodes for tse5 (1169-1229)Available SinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKTop::sptse5-CT (1169-1281)
Plasmid#192950PurposeFor membrane insertion study; Expresses sptse5-CT (1169-1281) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminusDepositorInsertsptse5-CT (1169-1281)
TagsPelB signal peptideExpressionBacterialMutationencodes for tse5 (1169-1281)Available SinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKTop::sptse5-CT (1169-1269)
Plasmid#192949PurposeFor membrane insertion study; Expresses Tse5-CT (1169-1269)/PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminusDepositorInsertsptse5-CT (1169-1269)
TagsPelB signal peptideExpressionBacterialMutationencodes for tse5 (1169-1269)Available SinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKTop::sptse5-CT (1169-1300)
Plasmid#192951PurposeFor membrane insertion study; Expresses sptse5-CT (1169-1300) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminusDepositorInsertsptse5-CT (1169-1300)
TagsPelB signal peptideExpressionBacterialMutationencodes for sptse5 (1169-1300)Available SinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-dTLN1 intron
Plasmid#176224PurposeA vector for the CRISPR-Cas9 system targeting an intron of dog TLN1 (talin1)DepositorInsertA gRNA targeting the dog TLN1 gene and the cDNA of Cas9 (TLN1 )
UseCRISPRExpressionMammalianAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-dTLN1 exon
Plasmid#176033PurposeA vector for the CRISPR-Cas9 system targeting an exon of dog TLN1 (talin1)DepositorInsertA gRNA targeting the dog TLN1 gene and the cDNA of Cas9 (TLN1 )
UseCRISPRExpressionMammalianAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a
Plasmid#182126PurposepET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a in bacteriaDepositorInsert1N/2CxNLS-cMyc-NLP-cMyc AspCas12aen
Tags6xHisExpressionBacterialPromoterT7Available SinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
1N/2CxNLS-cMyc-NLP-cMyc AspCas12a
Plasmid#182121PurposepET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc AspCas12a in bacteriaDepositorInsert1N/2CxNLS-cMyc-NLP-cMyc AspCas12a
Tags6xHisExpressionBacterialPromoterT7Available SinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a
Plasmid#182124PurposepET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a in bacteriaDepositorInsert1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a
Tags6xHisExpressionBacterialPromoterT7Available SinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
ppil2.280.457.Y389H
Plasmid#137658PurposeExpresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.DepositorAvailable SinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
ppil2.280.457.Y389W
Plasmid#137657PurposeExpresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.DepositorAvailable SinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNL-CEF-IgG1
Plasmid#187010PurposeExpression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgG1DepositorInsertHuman anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgG1
UseLentiviralAvailable SinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GFP-IRES-hSNCA (S129D)
Plasmid#185719PurposeAAV expression of GFP and human α-Synuclein with S129D mutation from hSyn promoterDepositorAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GFP-IRES-hSNCA (S129A)
Plasmid#185718PurposeAAV expression of GFP and human α-Synuclein with S129A mutation from hSyn promoterDepositorAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GFP-IRES-mSNCA(1-95)
Plasmid#185716PurposeAAV expression of GFP and C-terminal truncated (1-95 amino acid) mouse α-Synuclein from hSyn promoterDepositorAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-GFP11-RhoA
Plasmid#181860PurposePart of FluoSTEP-RhoA biosensor; co-express with cpPKN-mRuby2-GFP1-10 (Addgene #181859).DepositorAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHcRed-NPMMLF1nes-C1
Plasmid#131849PurposeExpresses the N-terminal nuclear export signal of HcRed-NPMMLF1 in mammalian cells through transient transfectionDepositorInsertNPM1 (NPM1 Human)
TagsHcRedExpressionMammalianMutationThis is a control deletion contruct of the cytop…Available SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
ETS2_pLENTI-CAG-IRES-GFP
Plasmid#177005PurposeMammalian lentiviral expression vector encoding ETS2DepositorAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
MOSMO_pcDNA6.2/EmGFP-Bsd
Plasmid#176975PurposeMammalian expression vector encoding MOSMO and EmGFP-BsdDepositorAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
CYYR1_pcDNA6.2/EmGFP-Bsd
Plasmid#176986PurposeMammalian expression vector encoding CYYR1 and EmGFP-BsdDepositorAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
CBR3_pcDNA6.2/EmGFP-Bsd
Plasmid#176961PurposeMammalian expression vector encoding CBR3 and EmGFP-BsdDepositorAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
CLDN8_pcDNA6.2/EmGFP-Bsd
Plasmid#176963PurposeMammalian expression vector encoding CLDN8 and EmGFP-BsdDepositorAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
FAM207A_pcDNA6.2/EmGFP-Bsd
Plasmid#176950PurposeMammalian expression vector encoding FAM207A and EmGFP-BsdDepositorAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
PX459_GRHL1-exon5
Plasmid#177763PurposesgRNA for CRISPR/Cas9-mediated deletion of human GRHL1DepositorInsertGRHL1 (GRHL1 Human)
UseCRISPRAvailable SinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
PX459_GRHL1-exon8
Plasmid#177764PurposesgRNA for CRISPR/Cas9-mediated deletion of human GRHL1DepositorInsertGRHL1 (GRHL1 Human)
UseCRISPRAvailable SinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Hygro(+)_GRHL1-R9A-L3F6H
Plasmid#177767PurposeOverexpression of 3xFLAG-6xHis tagged human GRHL1 (R9A mutation)DepositorAvailable SinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Hygro(+)_GRHL1-S95A-L3F6H
Plasmid#177768PurposeOverexpression of 3xFLAG-6xHis tagged human GRHL1 (S95A mutation)DepositorAvailable SinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Hygro(+)_GRHL1-K116A-L3F6H
Plasmid#177769PurposeOverexpression of 3xFLAG-6xHis tagged human GRHL1 (K116A mutation)DepositorAvailable SinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only