Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 15,983 results for: LEA

Clear keywords
Showing: 2501 - 2520 of 15983 results

  1. pAIP4_5GUQ

    Plasmid
    #234171
    Purpose
    Heterologous protein expression of ASCH domain-containing ribonuclease in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5GUQ
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234171-plasmid-map-sequence-id-464137

    Enlarge

  2. WT_H1-S1

    Plasmid
    #218400
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, and hnRNPA1 motif-rich region in first intron
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    218400-plasmid-map-sequence-id-442676

    Enlarge

  3. MUT_S1-N

    Plasmid
    #218398
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, neutral region in second intron, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218398-plasmid-map-sequence-id-433922

    Enlarge

  4. MUT_N-H1

    Plasmid
    #218397
    Purpose
    3-exon minigene, with neutral middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218397-plasmid-map-sequence-id-433918

    Enlarge

  5. WT_S7-H1

    Plasmid
    #218393
    Purpose
    3-exon minigene, with SRSF7 motif-rich middle exon, and hnRNPA1 motif-rich region in second intron
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218393-plasmid-map-sequence-id-433906

    Enlarge

  6. pQE80l B-CC-B

    Plasmid
    #196479
    Purpose
    Protein expression of SpyCatcher-ELP-CarHCC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher, and GRGD site linked with (GGGGS)2 before CarHCC
    Depositor
    Fei Sun
    Insert
    B-CC-B
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    196479-plasmid-map-sequence-id-387244

    Enlarge

  7. pJK636

    Plasmid
    #72466
    Purpose
    Produces Treponema denticola ATCC 35405 PurE with a TEV protease-cleavable N-terminal His6 tag (H6tTdPurE2)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72466-plasmid-map-sequence-id-133767

    Enlarge

  8. pHL1747

    Plasmid
    #53035
    Purpose
    PLtetO-1:ydeHlead::mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Han Lim
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7) and ydeHlead
    Expression
    Bacterial
    Promoter
    pLlacO-1 and pLtetO-1
    Available Since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  9. pHL1722

    Plasmid
    #53030
    Purpose
    PLtetO-1:glgClead::mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Han Lim
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7) and glgC leader
    Expression
    Bacterial
    Promoter
    pLlacO-1 and pLtetO-1
    Available Since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. pHL1745

    Plasmid
    #53033
    Purpose
    PLtetO-1:cstAlead::mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Han Lim
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7) and cstAlead
    Expression
    Bacterial
    Promoter
    pLlacO-1 and pLtetO-1
    Available Since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. pHL1724

    Plasmid
    #53031
    Purpose
    PLtetO-1:pgaAleadFull::mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Han Lim
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7) and pgaAleadFull
    Expression
    Bacterial
    Promoter
    pLlacO-1 and pLtetO-1
    Available Since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. pHL1914

    Plasmid
    #53042
    Purpose
    PconNoHindM2:hfqlead::gfp, PconNoHind:RBS(st3):tetR
    Depositor
    Han Lim
    Inserts
    GFP
    tetR
    Use
    Synthetic Biology
    Tags
    RBS(st3) and hfqlead
    Expression
    Bacterial
    Promoter
    PconNoHindM2 and pLlacO-1
    Available Since
    May 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. pAcGP67A - murine TfR

    Plasmid
    #12392
    Depositor
    Pamela Bjorkman
    Insert
    transferrin receptor (Tfrc Mouse)
    Tags
    6X-His tag, Factor Xa cleavage site, and leader p…
    Expression
    Insect
    Mutation
    See comments section
    Available Since
    Aug. 17, 2006
    Availability
    Academic Institutions and Nonprofits only
    12392-plasmid-map-sequence-id-274275

    Enlarge

  14. pExp-DsbA

    Plasmid
    #112562
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-DsbA(AA20-208) and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112562-plasmid-map-sequence-id-217223

    Enlarge

  15. pCWU50

    Plasmid
    #232318
    Purpose
    A conditional plasmid system where RepA, required for pCWU6-based plasmid replication, is regulated by an inducible fdx promoter and theophylline-responsive riboswitch
    Depositor
    Chenggang Wu
    Insert
    Pfdx-E
    Expression
    Bacterial
    Promoter
    fdx
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232318-plasmid-map-sequence-id-462281

    Enlarge

  16. pZP06

    Plasmid
    #232320
    Purpose
    pZP05 was constructed by cloning the oriFn-Pfdx-E-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, creating a conditional plasmid with replication controlled by Pfdx-E.
    Depositor
    Chenggang Wu
    Insert
    Pfdx-E
    Expression
    Bacterial
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232320-plasmid-map-sequence-id-462279

    Enlarge

  17. pPB-US-ECasE

    Plasmid
    #83961
    Purpose
    Piggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.
    Depositor
    Eleanor Chen
    Insert
    ERT2-spCas9-ERT2
    Expression
    Mammalian
    Mutation
    please see depositor comments below
    Promoter
    U6 (gRNA), EF1a (ERT2-Cas9-ERT2)
    Available Since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    83961-plasmid-map-sequence-id-214594

    Enlarge

  20. pCer-C3-Plk1-K82R

    Plasmid
    #68134
    Purpose
    Expression of Cerulean-tagged, kinase-dead Plk1 in mammalian cells
    Depositor
    Catherine Lindon
    Insert
    Polo-like kinase 1 (PLK1 Human)
    Tags
    Cerulean
    Expression
    Mammalian
    Mutation
    K82R kinase-dead version
    Promoter
    CMV
    Available Since
    Oct. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    68134-plasmid-map-sequence-id-123466

    Enlarge

Showing: 2501 - 2520 of 15983 results