We narrowed to 4,893 results for: PIR

1. CL7-LbCas12a

   Plasmid

   #164658

   Purpose

   Bacterial expression of LbCas12a using CL7/Im7

   Depositor

   Piyush Jain

   Article

   Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

   Insert

   LbCas12a
   

   Use

   Tags

   Thioredoxin, CL7, SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGBW-m4046768

   Plasmid

   #145763

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 E (envelope)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pAJM.847

   Plasmid

   #108524

   Purpose

   PhlFAM + PPhlF-YFP Reporter. DAPG sensor for use in non-Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   PhlFAM + PPhlF-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   PhlF: S50G S73I R95H A121V M137I N144D T155I R200C

   Promoter

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGBW-m4046959

   Plasmid

   #145722

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 ORF3a protein

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 ORF3a protein (ORF3a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 27, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pET28_mouse-pro-IL1B

   Plasmid

   #183386

   Purpose

   Overexpression of catalytic domain of inflammatory caspase in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   pro-IL1B (Il1b Mouse)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. OSCA1.2

   Plasmid

   #136593

   Purpose

   Expresses the mechanosensitive channel OSCA1.2 in mammalian cells

   Depositor

   Ardem Patapoutian

   Article

   Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

   Insert

   OSCA1.2 (AT4G22120 Mustard Weed)
   

   Use

   Tags

   pIRES2-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAJM.1642

   Plasmid

   #108535

   Purpose

   CinRAM + PCin-YFP Reporter. OHC14 sensor for use in non-Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   CinRAM + PCin-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   CinR: S72C V80I G114D G115D R137H K220R A224T *24…

   Promoter

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAJM.690

   Plasmid

   #108533

   Purpose

   PcaUAM + P3B5B-YFP Reporter. DHBA sensor for use in non-Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   PcaUAM + P3B5B-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   PcaU: K14N A165T N201H

   Promoter

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28_mouse-pro-IL18

   Plasmid

   #183389

   Purpose

   Overexpression of catalytic domain of inflammatory caspase in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   pro-IL18 (Il18 Mouse)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLG017 (AVAST type 1)

    Plasmid

    #157895

    Purpose

    Expresses the AVAST type 1 defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    AVAST type 1 (STAND ATPase with N-terminal serine protease)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAJM.884

    Plasmid

    #108536

    Purpose

    AcuRAM + PAcu-YFP Reporter. Acr sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    AcuRAM + PAcu-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    AcuR: E80K R154H L202M

    Promoter

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. LbCas12a-D156R

    Plasmid

    #213744

    Purpose

    For circular RNA-mediated prime editor using LbCas12a-D156R in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    LbCas12a
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    D156R

    Promoter

    CMV

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAC1430

    Plasmid

    #127981

    Purpose

    AMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    Cpf1
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    codon optimized for Aspergillus nidulans

    Promoter

    Aspergillus nidulans tef1 promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGBW-m4137384

    Plasmid

    #149543

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike) for VSV pseudotyping

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Human, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H. sapiens recode

    Promoter

    CMV

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pGBW-m4137383

    Plasmid

    #149541

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike) for VSV pseudotyping

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Human, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H. sapiens recode;18-aa Ctrunc

    Promoter

    CMV

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. LbdCas12a KRAB

    Plasmid

    #188503

    Purpose

    Expresses FLAG tagged LbdCas12a KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    EF1a

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. OSCA1.1

    Plasmid

    #136592

    Purpose

    Expresses the mechanosensitive channel OSCA1.1 in mammalian cells

    Depositor

    Ardem Patapoutian

    Article

    Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

    Insert

    OSCA1.1 (AT4G04340 Mustard Weed)
    

    Use

    Tags

    pIRES2-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAC1749

    Plasmid

    #127983

    Purpose

    AMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    Cpf1
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    codon optimized for Aspergillus nidulans

    Promoter

    Aspergillus nidulans tef1 promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGBW-m4046852

    Plasmid

    #145584

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp8

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. H6hSDHA

    Plasmid

    #113045

    Purpose

    Hetero expression of His-tagged human SDHA

    Depositor

    Edward A. Berry

    Article

    Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

    Insert

    H6hSDHA (SDHA Human)
    

    Use

    Tags

    His6 (MGSSHHHHHHSQDP-)

    Expression

    Bacterial

    Mutation

    Insert begins at amino acid 43

    Promoter

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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