We narrowed to 30,195 results for: REP

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A1-20B1(P004)

Plasmid

#184056

Purpose

This plasmid carries Yn situ preamplifier A1-20B1 sequence and is used for nickase based synthesis.

Depositor

C. Ron Yu

Article

Wu et al Cell Rep Methods. 2022 Apr 18;2(4):10020

Insert

A1-20B1 preamplifier

Use

Synthetic Biology

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pMK445 (BSD-P2A-BromoTag)

Plasmid

#214375

Purpose

BromoTag for N-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

BSD-P2A-BromoTag

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pX330A_D10A-1x2

Plasmid

#58772

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGEMT-PTE2A

Plasmid

#111773

Purpose

Quadcistronic construct: Co-express four genes by three 2A peptides -- P2A, T2A and E2A

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Type

Empty backbone

Use

Cloning intermediate

Promoter

T7

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST-EGFP-TARG1

Plasmid

#172586

Purpose

For transient expression of N-terminal GFP-tagged TARG1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

TARG1 (OARD1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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TOPO mKate2

Plasmid

#68441

Purpose

TOPO construct expressing mKate2 for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

mKate2

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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Ac5-STABLE2-puro

Plasmid

#208032

Purpose

Enables expression of 2 ORFs (with or without tags) and selection with puromycin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-puro with Actin5C promoter.

Depositor

Rosa Barrio, James Sutherland

Article

Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

Type

Empty backbone

Expression

Insect

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCDB179-DepoPETase

Plasmid

#218702

Purpose

Encodes codon optimized DepoPETase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

DepoPETase

Tags

10xHis

Expression

Bacterial

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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Tol2-UAS-Dendra2

Plasmid

#97145

Purpose

For Gal4/UAS expression system

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

Dendra2

Use

Zebrafish

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMK463 (BSD-P2A-mAB)

Plasmid

#214389

Purpose

mAID-BromoTag for N-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

BSD-P2A-mAID-BromoTag

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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PNCA-GFP-dYY1

Plasmid

#67791

Purpose

Use together with pCMV-intron and VSV-G to package MMLV reporter virus

Depositor

Stephen Goff

Article

Schlesinger et al Cell Rep. 2013 Jul 11;4(1):50-8. doi: 10

Insert

MMLV-LTR-GFP

Mutation

Deletion of LTR U3 YY1 binding domain

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only
pMK382

Plasmid

#214373

Purpose

Tet-OsTIR1(F74G) (AAVS1 insertion donor)

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

Tet-OsTIR1(F74G)

Expression

Mammalian

Promoter

Tet ON 3G promoter

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SM-FLuc0-pA

Plasmid

#210583

Purpose

Expresses SM(FLAG)-nonoptimal Firefly luciferase- pA

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-nonoptimal FLuc

Use

Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMK465 (mAB-Hygro)

Plasmid

#214391

Purpose

mAID-BromoTag for C-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

mAID-BrpmpTag-Hygro

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMK449 (BromoTag-mClover-BSD)

Plasmid

#214379

Purpose

BromoTag-mClover for C-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

BromoTag-mClover-BSD

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Flag Rab12 Q101L

Plasmid

#86788

Purpose

mammalian expression of Rab12 Q101L, constitutively GTP-bound mutant

Depositor

Peter McPherson

Article

Xu et al EMBO Rep. 2015 Jun;16(6):709-18. doi: 10

Insert

Rab12 (RAB12 Human)

Tags

Flag

Expression

Mammalian

Mutation

Q101L, constitutively GTP-bound mutant

Promoter

CMV

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pMK486 (Hygro-P2A-2mAID)

Plasmid

#214398

Purpose

2mAID for C-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

Hygro-P2A-2mAID

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMK464 (mAB-Neo)

Plasmid

#214390

Purpose

mAID-BromoTag for C-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

mAID-BromoTag-Neo

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMK448 (BromoTag-BSD)

Plasmid

#214378

Purpose

BromoTag for C-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

BromoTag-BSD

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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4xPylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)

Plasmid

#174529

Purpose

Dual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiency

Depositor

Yu-Hsuan Tsai

Article

Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

Inserts

PylS
PylT

Tags

Flag

Expression

Mammalian

Promoter

U6

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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