We narrowed to 5,792 results for: actin
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Plasmid#39461DepositorInsertmCD8-T2A-Gal4
PromoteractinAvailable sinceSept. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAG-NCad-B3
Plasmid#191668PurposeddFP-B3-inserted mouse N-cadherinDepositorInsertN-cadherin
TagsHA and ddFP-B3ExpressionMammalianPromoterChicken β-actin promoterAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro
Plasmid#216246PurposeExpress Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integrationDepositorPublicationInsertCas9 and sgAAVS1.5
UseCRISPR; Human safe harbor locus (a avs1) site-spe…ExpressionMammalianPromoterCAGAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CBE4max-SpRYc-P2A-EGFP
Plasmid#208336PurposeCAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYcDepositorInserthuman codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP
UseCRISPRTagsP2A-EGFPExpressionMammalianMutationSc++ (1-1119), SpRY (1111-1368), D10APromoterCAGAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-Oct4-p2a-Klf4-IRES-Sox2-17
Plasmid#193298Purposeepisomal OKS* (expressing mouse Oct4 + Klf4 + super-Sox) for generation of integration-free iPSCsDepositorExpressionMammalianMutationSox2-17 is engineered highly cooperative chimeric…PromoterCAGAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Stargazin-mEGFP-iLID
Plasmid#178523PurposeExpresses Stargazin-mEGFP-iLID in mammalian cellsDepositorInsertStargazin-mEGFP-iLID
ExpressionMammalianPromoterCAGAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB-PE
Plasmid#162796PurposeAll-in-one prime editor piggyBac transposonDepositorInsertSpCas9_ H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
UseCRISPR and Synthetic Biology; Piggybac transposonTagsSV40 NLSExpressionMammalianPromoterCAGAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG_Xph20-mRuby2-CCR5TC
Plasmid#135531PurposeEncodes a specific PSD-95 binder (Xph20) fused to mRuby2, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domainDepositorInsertXph20
TagsmRuby2ExpressionMammalianPromoterCAGAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Tol2-CAG::Cytbow
Plasmid#158991PurposeMulticolor cytoplasmic cell labeling with a genome-integrating Tol2 transposon vector (randomly expressing 3 tdTomato, mCerulean or mEYFP upon Cre recombination)DepositorInsertCytbow
TagsThe default expressed EBFP2 is fused to H2BExpressionMammalianPromoterCAGAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
LiOn-CAG∞IRFP
Plasmid#154017PurposeVector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein IRFP670 from a CAG promoter upon action of the piggyBac transposaseDepositorInsertIRFP670
ExpressionMammalianPromoterCAGAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-GCaMP6f-P2A-PAmCherry
Plasmid#133412PurposeFluorescent reporter for calcium imaging and photoactivation.DepositorInsertGCaMP6f-P2A-PAmCherry
UseAAVExpressionMammalianPromoterCAGAvailable sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-DDdCas9VP192-T2A-GFP-IRES-Neo
Plasmid#102885PurposePiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-T2A-GFP-IRES-Neo
UseCRISPRExpressionMammalianMutationD10A, H840APromoterCAGAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.CAG.SF-iGluSnFR.S72A
Plasmid#106200PurposeWeak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.S72A
UseAAVMutationGltI: S72APromoterCAGAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGP-AAV-CAG-FLEX-jGCaMP7c variant 1513-WPRE
Plasmid#105323PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre-dependent expressionDepositorInsertjGCaMP7c variant 1513
UseAAV and Cre/LoxTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianPromoterCAGAvailable sinceJan. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-PHFF
Plasmid#100280PurposeExpression vector for phycocyanobilin (PCB) synthesis in mammalian cells.DepositorInsertMTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
TagsFLAG, T7 and Mitochondria-targeting sequence (MTS…ExpressionMammalianMutationAll genes are codon-optimized for expression in h…PromoterCAG promoterAvailable sinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
RV-Cag-Dio-EYFP-P2A-cMaf
Plasmid#98174PurposeRetroviral vector encoding Cre-dependent expression of EYFP-P2A-MafDepositorAvailable sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
CAG-Otx2-IRES-GFPd2
Plasmid#73996PurposePlasmid expressing Otx2(isoform b)-IRES-GFPd2DepositorAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AMPH1-BAR-mCherry
Plasmid#85130PurposeBAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherryDepositorInsertBAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherry (AMPH Human)
TagsmCherryExpressionMammalianPromoterCAGAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPB-CAG-3xFLAG-empty-pgk-hph
Plasmid#48754PurposePiggyBac backbone, CAG promoter, Hygromycin selection, N-terminal 3xFLAG tagDepositorTypeEmpty backboneUsePiggybacExpressionMammalianPromoterCAGAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CAG sHRPa-FRB-pre mGRASP
Plasmid#73145PurposeLarge fragment of split HRP and FRB fused to the extracellular terminus of the "pre mGRASP" scaffold published by Magee, in which the extracellular domain of NRX is replaced by portions of CD4.DepositorInsertsHRPa-FRB-pre mGRASP
TagsFlag tagExpressionMammalianPromoterCAGAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only