We narrowed to 22,775 results for: crispr

1. pGES201

   Plasmid

   #190197

   Purpose

   For CRISPR-Cas9 mediated gene editing in soybean, soybean elongation factor 1A promoter (pM4)-Cas9, GmU6-sgRNA, Basta selection

   Depositor

   Yuefeng Guan

   Article

   Bai et al Plant Biotechnol J. 2020 Mar;18(3):721-7

   Insert

   spCas9
   

   Use

   CRISPR

   Tags

   3xFlag

   Expression

   Plant

   Mutation

   plant-codon optimized

   Promoter

   Glycine max elongation factor 1A(pM4)

   Available since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX458-DsRed2

   Plasmid

   #112219

   Purpose

   Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2

   Depositor

   Yinming Liang

   Article

   Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

   Insert

   DsRed2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   DsRed2 contains a silent nucleotide substitution …

   Promoter

   Available since

   July 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro

   Plasmid

   #42333

   Purpose

   This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   Available since

   March 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E1B

   Plasmid

   #64221

   Purpose

   Expression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry and to Ad4 E1B55K via P2A

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-mCherry-P2A-E1B

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-R26-FLEX-mStr

   Plasmid

   #135619

   Purpose

   Donor vector for genomic targeting of a CAG-FLEX-mStrawberry cassette to the mouse Rosa26 locus

   Depositor

   Lin He

   Article

   Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

   Insert

   CAG-FLEX-mStrawberry flanked by Rosa26 homology arms (Gt(ROSA)26Sor Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMVp-dCas9-3xNLS-VP64 (Construct 1)

   Plasmid

   #55195

   Purpose

   Expresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector.

   Depositor

   Timothy Lu

   Article

   Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

   Insert

   dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   VP64

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/hUBC

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSP712

   Plasmid

   #65768

   Purpose

   Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Bacterial

   Mutation

   D10A and H840A mutations in Cas9

   Promoter

   T7 (x2)

   Available since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pscAAV-Sox2-mStr

   Plasmid

   #135617

   Purpose

   Donor vector for genomic targeting of a P2A-mStrawberry cassette to the 3' end of the mouse Sox2 locus

   Depositor

   Lin He

   Article

   Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

   Insert

   P2A-mStrawberry flanked by Sox2 homology arms (Sox2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   P2A-mStrawberry

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pY31

   Plasmid

   #84746

   Purpose

   Expresses huLbCpf1-P2A-puro and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huLbCpf1
   puromycin resistance gene
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pY095

    Plasmid

    #84744

    Purpose

    Expresses huLbCpf1-T2A-GFP and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Inserts

    huLbCpf1
    GFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEJS581-pCSDest2-AcrIIC4Hpa-FLAG-NLS

    Plasmid

    #113436

    Purpose

    Expresses a FLAG/NLS tagged type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

    Insert

    type II-C anti-CRISPR
    

    Use

    CRISPR

    Tags

    FLAG/NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-CAG-DDdCas9VPPH-T2A-GFP-IRES-Neo

    Plasmid

    #102888

    Purpose

    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV CMV-driven AcrIIA4-2xmiR-122 target sites

    Plasmid

    #120298

    Purpose

    AAV vector for expression of AcrIIA4 with two miR-122 binding sites

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    AcrIIA4-2xmiR-122 binding sites
    

    Use

    AAV and CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

14. pGEL033

    Plasmid

    #137902

    Purpose

    STU2.0 rAPOBEC1-SpyCas9(D10A) for plant genome base editing

    Depositor

    Yong Zhang

    Article

    Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-Flag-LmoCas6

    Plasmid

    #126485

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas6
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. H513 px330.sgActin.UTR.2

    Plasmid

    #138176

    Purpose

    CRISPR SONIC: Encodes codon-optimized SpCas9 and gRNA for targeting 3' UTR of β-actin.

    Depositor

    Wen Xue

    Article

    Mou et al Genome Med. 2019 Apr 16;11(1):21. doi: 1

    Insert

    sgActin (Actb Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-Flag-LmoCas8b2

    Plasmid

    #126482

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B Cas8b2 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas8b2
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-Flag-LmoCas7

    Plasmid

    #126483

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B Cas7 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas7
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-Flag-LmoCas5

    Plasmid

    #126484

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B Cas5 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas5
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUDP012

    Plasmid

    #101167

    Purpose

    E. coli/S. cerevisiae shuttle vector carrying amdS andSpcas9D147Y P411T and expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 and in S. pastorianus (HH-gRNASeILV6-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Insert

    HH-gRNA-HDV targetting SeILV6 in S. pastorianus
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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