We narrowed to 157,337 results for: Des

1. pORANGE GFP-Nlgn3 KI

   Plasmid

   #131501

   Purpose

   Endogenous tagging of Neuroligin-3: N-terminal (amino acid position: T37)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Nlgn3, Nlgn3 Rat, Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pORANGE GFP-Syt7 KI

   Plasmid

   #131488

   Purpose

   Endogenous tagging of Synaptotagmin-7: N-terminal (amino acid position: P5)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Syt7, Syt7 Rat, Mouse)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cre-p2a-tdTomato

   Plasmid

   #176837

   Purpose

   Express Cre in mammalian cells and enable FACS sorting Cre expressing cells by tdTomato expression

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

   Insert

   Cre-P2A-tdTomato
   

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Nov. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pORANGE GFP-CADPS KI

   Plasmid

   #131482

   Purpose

   Endogenous tagging of CAPS1: N-terminal (amino acid position: S39)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Cadps Rat)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTRY-GFP

   Plasmid

   #15301

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   hrGFP
   

   Expression

   Mammalian

   Available Since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pFUGW ORANGE Tubb3-GFP KI _ mCherry-KASH

   Plasmid

   #131508

   Purpose

   Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Tubb3, Tubb3 Rat, Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hUBC

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJEP304-pAAV-EFS-dSaCas9-VP64-pA

   Plasmid

   #113679

   Purpose

   A EFS driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted region

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   de-catalyzed SaCas9
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   NLS and VP64

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV ORANGE Gria1-HaloTag KI

   Plasmid

   #139655

   Purpose

   Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and HaloTag donor
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pORANGE CACNG8-GFP KI #2

   Plasmid

   #131474

   Purpose

   Endogenous tagging of Tarpγ8: Intramolecular (amino acid position: A408)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Cacng8 Rat)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pORANGE GFP-Clta KI

    Plasmid

    #131483

    Purpose

    Endogenous tagging of Clathrin light chain α: N-terminal (amino acid position: M1)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Clta Rat)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-IP3KB

    Plasmid

    #32528

    Depositor

    Christophe Erneux

    Article

    Dewaste et al Biochem J. 2003 Aug 15;374(Pt 1):41-9.

    Insert

    IP3KB (ITPKB Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pENTRY-YFP

    Plasmid

    #15302

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    YFP
    

    Use

    Gateway entry

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

13. GFP-IP3KA

    Plasmid

    #133582

    Purpose

    Expresses IP3KA GFP tagged

    Depositor

    Christophe Erneux

    Article

    Dewaste et al Biochem J. 2003 Aug 15;374(Pt 1):41-9.

    Insert

    Human inositol 1,4,5-trisphosphate 3-kinase A GFP tagged (ITPKA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

14. pORANGE Shank1-GFP KI

    Plasmid

    #131500

    Purpose

    Endogenous tagging of Shank1: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Shank1, Shank1 Rat, Mouse)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pORANGE Rims2-GFP KI

    Plasmid

    #131495

    Purpose

    Endogenous tagging of RIM2: C-terminal (amino acid position: S1551)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Rims2, Rims2 Rat, Mouse)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJEP302-pAAV-CMV-dSaCas9-KRAB-pA

    Plasmid

    #113677

    Purpose

    A CMV driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    de-catalyzed SaCas9
    

    Use

    AAV and CRISPR

    Tags

    KRAB and NLS

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pORANGE Shank2-GFP KI

    Plasmid

    #131496

    Purpose

    Endogenous tagging of Shank2: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Shank2, Shank2 Rat, Mouse)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJEP309-pAAV-EFS-dSaCas9-KRAB-Dio-pA

    Plasmid

    #113686

    Purpose

    An EFS driven inverted de-catalyzed SaCas9 fused to the transcription repressor KRAB. dSaCas9-KRAB is floxed to render the system cre-dependent.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    de-catalyzed SaCas9
    

    Use

    AAV, CRISPR, and Cre/Lox

    Tags

    KRAB

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJEP308-pAAV-EFS-dSaCas9-VP64-Dio-pA

    Plasmid

    #113685

    Purpose

    An EFS driven inverted de-catalyzed SaCas9 fused to the transcription activator VP64. dSaCas9-VP64 is floxed to render the system cre-dependent.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    inverted de-catalyzed SaCas9
    

    Use

    AAV, CRISPR, Cre/Lox, and Synthetic Biology

    Tags

    NLS and VP64

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJEP305-pAAV-EFS-dSaCas9-KRAB-pA

    Plasmid

    #113681

    Purpose

    A EFS driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    de-catalyzed SaCas9
    

    Use

    AAV and CRISPR

    Tags

    KRAB and NLS

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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