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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 165,652 results for: addgene

Showing: 2521 - 2540 of 165652 results

  3. pU6-(BsaI)_CBh-UC-Cas9-T2A-mCherry

    Plasmid
    #135014
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to C-terminus of Cas9, linked to mCherry via a T2A peptide
    Depositor
    Yosef Shaul
    Article
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Insert
    humanized S. pyogenes Cas9
    Tags
    3x Flag, 3xFLAG (N terminal on insert), NLS, and …
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the C-termi…
    Promoter
    CBh
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    135014-plasmid-map-sequence-id-267599

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  4. pU6-(BsaI)_CBh-UN-Cas9-T2A-mCherry

    Plasmid
    #135013
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9, linked to mCherry via a T2A peptide
    Depositor
    Yosef Shaul
    Article
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Insert
    humanized S. pyogenes Cas9
    Tags
    3x Flag, 3xFLAG (N terminal on insert), NLS, and …
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the N-termi…
    Promoter
    CBh
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    135013-plasmid-map-sequence-id-267601

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  8. GG-EBNA-MIR302-3g-EEA-2g-PGK-Puro

    Plasmid
    #201677
    Purpose
    EBNA episome plasmid for U6 promoter-driven expression of 3 gRNAs targeting miRNA302/367 (Addgene #201960) and 2 gRNAs targeting EEA-motif (Addgene #102898). Includes PGK-puro selection cassette
    Depositor
    Timo Otonkoski
    Article
    Sokka et al Stem Cell Reports. 2022 Feb 8;17(2):413-
    Insert
    MIR302-3g-EEA-2g-PGK-Puro
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    201677-plasmid-map-sequence-id-395330

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  9. pMBP-HsSyx3

    Plasmid
    #179287
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Tags
    His6 + mature maltose binding protein + TEV prote…
    Expression
    Bacterial
    Mutation
    residues 393-792 from accession number NP_0010361…
    Promoter
    T7
    Available Since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179287-plasmid-map-sequence-id-354925

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  10. pMBP-HsSyx-375-792

    Plasmid
    #179291
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Tags
    His6 + mature maltose binding protein + TEV prote…
    Expression
    Bacterial
    Mutation
    residues 375-792 from accession number NP_0010361…
    Promoter
    T7
    Available Since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179291-plasmid-map-sequence-id-354915

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  12. pRS418

    Plasmid
    #11256
    Depositor
    Fred Cross
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    Feb. 15, 2006
    Availability
    Academic Institutions and Nonprofits only
    11256-plasmid-map-sequence-id-6930

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  13. pRS428

    Plasmid
    #11257
    Depositor
    Fred Cross
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    Feb. 15, 2006
    Availability
    Academic Institutions and Nonprofits only
    11257-plasmid-map-sequence-id-6931

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  14. CAG pShuttle

    Plasmid
    #13427
    Depositor
    Ronald Kahn
    Type
    Empty backbone
    Use
    Adenoviral
    Expression
    Mammalian
    Available Since
    Nov. 1, 2006
    Availability
    Academic Institutions and Nonprofits only
    13427-plasmid-map-sequence-id-258367

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  15. U6 pShuttle

    Plasmid
    #13428
    Depositor
    Ronald Kahn
    Type
    Empty backbone
    Use
    Adenoviral
    Expression
    Mammalian
    Available Since
    Nov. 1, 2006
    Availability
    Academic Institutions and Nonprofits only
    13428-plasmid-map-sequence-id-263315

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  16. pTN186

    Plasmid
    #34890
    Depositor
    Mitsuyasu Hasebe
    Type
    Empty backbone
    Use
    Moss targeting
    Available Since
    Feb. 28, 2012
    Availability
    Academic Institutions and Nonprofits only

  18. PGKFlpebpA

    Plasmid
    #13441
    Depositor
    Philippe Soriano
    Insert
    PGKFlpebpA
    Expression
    Mammalian
    Available Since
    Nov. 16, 2006
    Availability
    Academic Institutions and Nonprofits only
    13441-plasmid-map-sequence-id-7627

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  19. CbetaS-pA

    Plasmid
    #32104
    Depositor
    Michael Cole, Yardena Samuels
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    cmv
    Available Since
    Sept. 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  20. JDS88

    Plasmid
    #40319
    Depositor
    Keith Joung
    Type
    Empty backbone
    Tags
    3XFLAG and FOK1 KKR (+) heterodimer
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 26, 2012
    Availability
    Academic Institutions and Nonprofits only
    40319-plasmid-map-sequence-id-51987

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Showing: 2521 - 2540 of 165652 results