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EasyFusion T2A-H2B-miRFP703

Plasmid

#113097

Purpose

General donor vector with T2A-H2B-miRFP703 coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

T2A-H2B-miRFP703

Use

Synthetic Biology

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-SV40-puro-EF1a-HA-NLGN1-bc

Plasmid

#200167

Purpose

Expresses HA-tagged human NLGN1

Depositor

Paul Blainey

Article

Le et al Cell Rep. 2025 Jun 24;44(7):115889. doi:

Insert

Neuroligin-1 (NLGN1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

EF1a

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTOX4

Plasmid

#127449

Purpose

Encodes YhaV toxin and amilCP chromoprotein. CAM-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Inserts

yhaV toxin
amilCP chromoprotein

Expression

Bacterial

Mutation

Codon optimized from protein sequence in FPbase

Promoter

pRha and tac

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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pRL278

Plasmid

#70256

Purpose

sacB plasmid with polylinker; kanamycin/neomycin resistance: used as a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion seq.

Depositor

Peter Wolk

Article

Cai et al J Bacteriol. 1990 Jun;172(6):3138-45.

Type

Empty backbone

Use

Conditionally lethal

Available Since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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EasyFusion T2A-H2B-TagBFP

Plasmid

#113086

Purpose

General donor vector with T2A-H2B-TagBFP coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

T2A-H2B-TagBFP

Use

Synthetic Biology

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pKm-HABE

Plasmid

#170332

Purpose

a modularized single-plasmid adenosine base editing (A-to-G) tool for Sinorhizobium meliloti

Depositor

Deqiang Duanmu

Article

Wang et al Front Microbiol. 2021 Jun 18;12:686008.

Insert

Rhizobium codon-optimized ABEs

Use

CRISPR

Mutation

Cas9n-D10A

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR APLP1 shRNA 2

Plasmid

#30157

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al Neural Dev. 2008 Jun 23;3:15. doi: 10.11

Insert

APLP1 shRNA 2

Expression

Mammalian

Available Since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
pGCDNsam-Foxa1-IRES-GFP

Plasmid

#33007

Depositor

Atsushi Suzuki

Article

Sekiya et al Nature. 2011 Jun 29;475(7356):390-3. doi

Insert

forkhead box A1 (Foxa1 Mouse)

Use

Retroviral

Available Since

March 6, 2012

Availability

Academic Institutions and Nonprofits only

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pNM3689

Plasmid

#154111

Purpose

miniMos single component RMCE landing site vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

loxP::SEC::loxP::mex-5p::FLP::sl2::nNeonGreen::glh-2 3'::rpl-28p::FRT::GFP::his-58::unc-54 3'::FRT3

Use

Minimos

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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hRIP3 GFP wt

Plasmid

#41387

Depositor

Francis Chan

Article

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP3 (RIPK3 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only

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pEC2483 (pSpy1C)

Plasmid

#220248

Purpose

replicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expression

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pTOX5

Plasmid

#127450

Purpose

Encodes MqsR toxin and amilCP chromoprotein. CAM-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Inserts

mqsR toxin
amilCP chromoprotein

Expression

Bacterial

Mutation

Codon optimized from protein sequence in FPbase

Promoter

pRha and tac

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-mNeon-TurboID

Plasmid

#186610

Purpose

Third generation lentiviral vector expressing mNeonGreen fused to V5-TurboID

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

mNeon-V5-TurboID

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Mutation

For reference see Branon et al., 2018

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pBBK28 pHomL-TDH3p-GFPdropout-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179012

Purpose

Parent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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DAGR

Plasmid

#14865

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

DAG reporter (Prkcb Rat)

Tags

CFP and YFP

Expression

Mammalian

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pBS-HA::UPRT

Plasmid

#47110

Purpose

for subcloning UPRT into expression vectors

Depositor

Mike Cleary, Chris Doe

Article

Miller et al Nat Methods. 2009 Jun;6(6):439-41. doi:

Insert

uracil phosphoribosyltransferase

Tags

HA

Available Since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pC1-CMV-DAAO-NES

Plasmid

#141107

Purpose

Expresses DAAO with nuclear export signal in mammalian cells

Depositor

Vsevolod Belousov

Article

Bogdanova et al Curr Protoc Chem Biol. 2017 Jun 19;9(2):

Insert

DAAO-NES

Tags

Nuclear export signal

Expression

Mammalian

Mutation

deleted DAAO amino acids 367-368

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAVEC2_N-TurboID

Plasmid

#231293

Purpose

For transiently expressing fusion proteins with N terminal TurboID for proximity labeling in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

TurboID

Expression

Plant

Mutation

N/A

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC2_N-mEGFP

Plasmid

#231287

Purpose

For transiently expressing fusion proteins with N terminally tagged mEGFP in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mEGFP

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC2_N-mCitrine

Plasmid

#231289

Purpose

For transiently expressing fusion proteins with N terminally tagged mCitrine in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mCitrine

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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