We narrowed to 11,913 results for: pcDNA

1. pcDNA3-RIIα-μDpTT

   Plasmid

   #182327

   Purpose

   PKA RIIα subunit-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

   Depositor

   Jin Zhang

   Article

   Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

   Insert

   RIIalpha-μDronpa-TagRFP-T (PRKAR2A Human)
   

   Use

   Tags

   Full-length human PKA type 2alpha regulatory subu…

   Expression

   Mammalian

   Mutation

   Dronpa contains H193T mutation.

   Promoter

   CMV

   Available since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3-H2B-μDpTT

   Plasmid

   #182328

   Purpose

   Histone H2B-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

   Depositor

   Jin Zhang

   Article

   Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

   Insert

   H2B-μDronpa-TagRFP-T (H2BC3 Human)
   

   Use

   Tags

   Full-length human histone H2B

   Expression

   Mammalian

   Mutation

   Dronpa contains H193T mutation.

   Promoter

   CMV

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-Lifeact-Dronpa

   Plasmid

   #182330

   Purpose

   Actin-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

   Depositor

   Jin Zhang

   Article

   Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

   Insert

   Lifeact-Dronpa
   

   Use

   Tags

   N-terminal 17 amino acids from budding yeast Abp1…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-Lyn-μDpTT

   Plasmid

   #182321

   Purpose

   Plasma membrane-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

   Depositor

   Jin Zhang

   Article

   Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

   Insert

   Lyn-μDronpa-TagRFP-T
   

   Use

   Tags

   N-terminal targeting sequence from Lyn kinase

   Expression

   Mammalian

   Mutation

   Dronpa contains H193T mutation.

   Promoter

   CMV

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-CYP450-μDpTT

   Plasmid

   #182325

   Purpose

   Endoplasmic reticulum-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

   Depositor

   Jin Zhang

   Article

   Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

   Insert

   DAKAP-μDronpa-TagRFP-T
   

   Use

   Tags

   N-terminal 27 amino acids from CYP450

   Expression

   Mammalian

   Mutation

   Dronpa contains H193T mutation.

   Promoter

   CMV

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-Lifeact-μDpTT

   Plasmid

   #182322

   Purpose

   Actin-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

   Depositor

   Jin Zhang

   Article

   Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

   Insert

   Lifeact-μDronpa-TagRFP-T
   

   Use

   Tags

   N-terminal 17 amino acids from budding yeast Abp1…

   Expression

   Mammalian

   Mutation

   Dronpa contains H193T mutation.

   Promoter

   CMV

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA4TO-24xGCN4_v4-kif18b

   Plasmid

   #74934

   Purpose

   Translation reporter that lacks PP7 sites in the 3'UTR

   Depositor

   Marvin Tanenbaum

   Article

   Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

   Insert

   24xGCN4_v4-kif18b
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3 HA Sam68 deltaKH

   Plasmid

   #17688

   Depositor

   David Shalloway

   Article

   Lin et al J Biol Chem. 1997 Oct 24. 272(43):27274-

   Insert

   Sam68 (Khdrbs1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   aa 157-256 deleted (KH domain)

   Promoter

   Available since

   April 24, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA5-FRT-TO-Nsp16

   Plasmid

   #157723

   Purpose

   mammalian expression of untagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp16 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pcDNA5/FRT/TO V5 DNAJB2b

    Plasmid

    #19524

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    DNAJB2b (DNAJB2 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-ShhN-Ren

    Plasmid

    #37677

    Depositor

    Philip Beachy

    Article

    Ma et al Cell. 2002 Oct 4;111(1):63-75.

    Insert

    shh (Shh Mouse)
    

    Use

    Tags

    renilla luciferase

    Expression

    Mammalian

    Mutation

    soluble form (see comments)

    Promoter

    CMV

    Available since

    Nov. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA5-FRT-TO-Nsp10

    Plasmid

    #157706

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp10 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pcDNA5-FRT-TO-FH-eGFP

    Plasmid

    #157734

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged eGFP under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-eGFP
    

    Use

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-HA-ERK2 D319N

    Plasmid

    #8979

    Depositor

    John Blenis

    Article

    Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

    Insert

    ERK2 (Mapk1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D319N

    Promoter

    Available since

    Oct. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3-AktAR2-NES

    Plasmid

    #175011

    Purpose

    Cytosol-targeted AktAR2.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    AktAR-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA-HA-Peli1 (mouse)

    Plasmid

    #44037

    Depositor

    Shao-Cong Sun

    Article

    Chang et al Nat Immunol. 2009 Oct;10(10):1089-95. do

    Insert

    pellino 1 (Peli1 Mouse)
    

    Use

    Tags

    1xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.1(+)- e18 LOF

    Plasmid

    #131729

    Purpose

    Expresses a loss of function mutant of an engineered variant of RAD18 ("e18")

    Depositor

    Alberto Ciccia

    Article

    Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

    Insert

    e18/D221A (Loss of function mutant of RAD18 with SAP domain deleted and UBZ point mutation) (RAD18 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5-FRT-TO-Nsp9-HF

    Plasmid

    #157705

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp9 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp9-HF (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pcDNA3.1-mPCSK3 H194L

    Plasmid

    #122689

    Purpose

    Expresses mutated mouse PCSK3 in mammalian cells (histidine at position 194 mutated to leucine via site directed mutagenesis)

    Depositor

    Liming Pei

    Article

    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

    Insert

    Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed Histidine 194 to Leucine

    Promoter

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-mDIS3-FLAG

    Plasmid

    #60046

    Purpose

    Mammalian expression of FLAG-tagged mouse DIS3

    Depositor

    Zissimos Mourelatos

    Article

    Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

    Insert

    DIS3 (Dis3 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 12, 2014

    Availability

    Academic Institutions and Nonprofits only