4,181 results

1. pFR_HCV_xb

   Plasmid

   #11510

   Depositor

   Phil Sharp

   Article

   Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

   Insert

   Firefly luciferase, Hepatitis C Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
   

   Use

   Luciferase

   Available Since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-DIO-mCherry

   Plasmid

   #50459

   Purpose

   Double floxed mCherry under the control of human synapsin promoter

   Depositor

   Bryan Roth

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   mCherry
   

   Use

   AAV

   Tags

   N/A

   Promoter

   human Synapsin 1

   Available Since

   March 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pORANGE Cloning template vector

   Plasmid

   #131471

   Purpose

   Cloning template form ORANGE method based knock-in constructs

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Type

   Empty backbone

   Tags

   HA

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ub-R-GFP

   Plasmid

   #11939

   Depositor

   Nico Dantuma

   Article

   Dantuma et al Nat Biotechnol. 2000 May . 18(5):538-43.

   Insert

   Ub-R-GFP
   

   Expression

   Mammalian

   Mutation

   Ubiquitin fused to N-terminus of GFP. Arginine at…

   Available Since

   June 22, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMK292 (mAID-mCherry2-NeoR)

   Plasmid

   #72830

   Purpose

   C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-mCherry2-Neo
   

   Use

   CRISPR

   Tags

   mAID-mCherry2

   Expression

   Mammalian

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-EF1a-IRES-Blast

   Plasmid

   #85133

   Purpose

   lentiviral backbone

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-FLEX-tdTomato

   Plasmid

   #28306

   Purpose

   Cre-dependent AAV-mediated expression of tdTomato under the CAG promoter

   Depositor

   Edward Boyden

   Has Service

   AAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   tdTomato
   

   Use

   AAV and Cre/Lox; Adeno-associated virus

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CAG

   Available Since

   Sept. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-RAM-d2TTA::TRE-EGFP-WPREpA

   Plasmid

   #84469

   Purpose

   pAAV-RAM-EGFP

   Depositor

   Yingxi Lin

   Article

   Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

   Insert

   EGFP
   

   Use

   AAV

   Promoter

   TRE

   Available Since

   Dec. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPomyces-2

   Plasmid

   #61737

   Purpose

   Streptomyces expression of codon-optimized Cas9 and custom gRNA

   Depositor

   Huimin Zhao

   Article

   Cobb et al ACS Synth Biol. 2014 Dec 8.

   Inserts

   sSpCas9
   gRNA cassette
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   BbsI-flanked lacZ cassette inserted in place of s…

   Promoter

   gapdhp(EL) and rpsL(XC)-BbsI

   Available Since

   Jan. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CaMKIIa-hM3D(Gq)-mCherry

    Plasmid

    #50476

    Purpose

    Gq-coupled hM3D DREADD fused with mCherry under the control of CaMKIIa promoter

    Depositor

    Bryan Roth

    Has Service

    AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    hM3D(Gq)-mCherry (CHRM3 Human)
    

    Use

    AAV

    Tags

    mCherry

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    CaMKIIa

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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