Purpose
All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1206a sequence CGAACATTTTTCCATGCGCT in yeast chromosome 12.
Insert
Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1206a
Available Since
April 5, 2017
Availability
Academic Institutions and Nonprofits only