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We narrowed to 23,988 results for: nes

Showing: 2541 - 2560 of 23988 results

  1. pC0048-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q)

    Plasmid
    #103864
    Purpose
    dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GSGGGGS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Promoter
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    103864-plasmid-map-sequence-id-199176

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  2. pC0039-CMV-dPspCas13b-GS-ADAR2DD(E488Q)

    Plasmid
    #103849
    Purpose
    dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA.
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Promoter
    CMV
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    103849-plasmid-map-sequence-id-199152

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  3. Ratiometric FPX sensor for ERK kinase activity

    Plasmid
    #60974
    Purpose
    For imaging of ERK kinase activity using a single polypeptide RA-WW domain-ERK substrate-B protein and free GA-NES.
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddRFP A-WW domain-ERK substrate-ddFP B
    Use
    Tags
    ERK substrate is before ddFP copy B, WW domain is…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. pAB1676 REPAIR.t1

    Plasmid
    #176319
    Purpose
    CMV-HIVNES-GS-dCas13bt1- (GGS)2-huADAR2dd(E488Q) (REPAIR.t1 expression)
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Inserts
    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Use
    CRISPR
    Tags
    HIV NES
    Expression
    Mammalian
    Mutation
    E488Q, only the deaminase domain (aa 276-702 are …
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176319-plasmid-map-sequence-id-345279

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  8. pKT-IDH1(R132H)-IRES-Katushka

    Plasmid
    #124257
    Purpose
    Expresses IDH1 with a R132H mutation and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Article
    Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii
    Insert
    Isocitrate dehydrogenase 1 (R132H) (IDH1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Arginine 132 is mutated to Histidine. This mutati…
    Promoter
    Available since
    Sept. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    124257-plasmid-map-sequence-id-252091

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  9. pUAP1

    Plasmid
    #63674
    Purpose
    Accepts DNA sequences via BpiI cloning sites, resulting in Level 0 standard parts for Golden Gate cloning. Parts can be released with a user-defined 4-base-pair, 5 prime overhangs using BsaI.
    Depositor
    Nicola Patron
    Article
    Patron et al New Phytol. 2015 Jul 14. doi: 10.1111/np
    Insert
    RFP cloning selection cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    63674-plasmid-map-sequence-id-106088

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  10. 4xNFkB Luc

    Plasmid
    #111216
    Purpose
    reporter gene
    Depositor
    Johannes A. Schmid
    Insert
    firefly Luciferase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    111216-plasmid-map-sequence-id-213082

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  11. pVenus-N1 (804)

    Plasmid
    #61854
    Purpose
    empty backbone for tagging proteins at the C-terminus with Venus
    Depositor
    Jonathon Pines
    Type
    Empty backbone
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61854-plasmid-map-sequence-id-375257

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  12. pcDNA3.1-xASIC1

    Plasmid
    #32793
    Depositor
    Cecelia Canessa
    Insert
    ASIC1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 1, 2011
    Availability
    Academic Institutions and Nonprofits only

  13. FUCas9GFP

    Plasmid
    #85555
    Purpose
    Expresses Cas9 with green fluorescent reporter
    Depositor
    Marco Herold
    Insert
    humanised S.pyogenes Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    hUBC
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85555-plasmid-map-sequence-id-169910

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  14. pEN_TT CVB3 A67G

    Plasmid
    #216788
    Purpose
    Gateway entry vector for the Kandolf strain of CVB3 with a VP4 A67G mutation
    Depositor
    Kevin Janes
    Insert
    CVB3
    Use
    Entry vector for gateway cloning
    Tags
    Expression
    Mutation
    A67G in VP4
    Promoter
    Available since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216788-plasmid-map-sequence-id-434924

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  15. FUCas9Cyan

    Plasmid
    #85554
    Purpose
    Expresses Cas9 with fluorescent Cyan reporter
    Depositor
    Marco Herold
    Insert
    humanised S.pyrogenes Cas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    hUBC
    Available since
    June 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    85554-plasmid-map-sequence-id-180892

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  16. pEN_TT luciferase-V5

    Plasmid
    #220360
    Purpose
    Gateway entry vector for an inducible V5-tagged luciferase
    Depositor
    Kevin Janes
    Insert
    luciferase
    Use
    Entry vector for gateway cloning
    Tags
    V5
    Expression
    Mutation
    Promoter
    Available since
    Oct. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    220360-plasmid-map-sequence-id-447352

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  17. pcDNA3 VP4-VP2

    Plasmid
    #216787
    Purpose
    Mammalian expression of the VP4-VP2 portion of the CVB3 Kandolf strain polyprotein
    Depositor
    Kevin Janes
    Insert
    VP4-VP2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    216787-plasmid-map-sequence-id-434923

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  18. EGFP-TRAF1

    Plasmid
    #111199
    Purpose
    fluorescent fusion protein
    Depositor
    Johannes A. Schmid
    Insert
    TRAF1 (TRAF1 Human)
    Use
    Tags
    EGFP-FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    111199-plasmid-map-sequence-id-213113

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  19. EYFP-TRAF1

    Plasmid
    #111200
    Purpose
    fluorescent fusion protein
    Depositor
    Johannes A. Schmid
    Insert
    TRAF1 (TRAF1 Human)
    Use
    Tags
    EYFP and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    111200-plasmid-map-sequence-id-212973

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  20. ECFP-TRAF1

    Plasmid
    #111198
    Purpose
    fluorescent fusion protein
    Depositor
    Johannes A. Schmid
    Insert
    TRAF1 (TRAF1 Human)
    Use
    Tags
    ECFP and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    111198-plasmid-map-sequence-id-213171

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Showing: 2541 - 2560 of 23988 results