We narrowed to 17,391 results for: puro

1. LentiU6-hASCL1 gRNA_multi1-3-MS2-Puro

   Plasmid

   #192682

   Purpose

   Lentiviral expression of multi gRNAs targeting hASCL1 promoter to activate human ASCL1 transcription

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   Human ASCL1 activating gRNAs #1,2,3 (ASCL1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTopo pLox hPGK-Puro pA pLox

   Plasmid

   #171048

   Purpose

   Empty donor template for CRISPR/Cas9 targeting of TERT RE's

   Depositor

   Agnel Sfeir

   Article

   Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCT-CMV-UBE3B-copGFP-Puro

   Plasmid

   #107342

   Purpose

   Expresses UBE3B fused to copGFP in mammalian cells.

   Depositor

   Robert Sobol

   Article

   Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

   Insert

   Ubiquitin-protein ligase E3B (UBE3B Human)
   

   Use

   Lentiviral

   Tags

   copGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 19, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pLVX-HA-UBE3B(C1036A)-IRES-Puro

   Plasmid

   #107348

   Purpose

   Expresses HA tagged UBE3B mutant C1036A in mammalian cells.

   Depositor

   Robert Sobol

   Article

   Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

   Insert

   UBE3B(C1036A) (UBE3B Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed Cysteine 1036 to Alanine

   Promoter

   CMV

   Available since

   April 19, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pLentiPGK Puro DEST JNKKTR EE Clover

   Plasmid

   #90239

   Purpose

   Lentiviral vector to express JNK KTR EE (mutant) mClover under PGK promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   JNK Kinase Translocation Reporter (EE mutant)
   

   Use

   Lentiviral

   Tags

   mClover

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX puro TRIM21 C54Y - GFP

   Plasmid

   #116942

   Purpose

   Expression of TRIM21 C54Y -GFP fusion

   Depositor

   Gaudenz Danuser

   Article

   Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

   Insert

   TRIM21 C54Y-GFP (TRIM21 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   C54Y

   Promoter

   Available since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1-puro U6 sgRNA SOX17 -177

   Plasmid

   #50925

   Purpose

   U6 driven sgRNA targeting Sox17 -177 bp from TSS

   Depositor

   Rene Maehr, Scot Wolfe

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   Sox17 -177 sgRNA (SOX17 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBABE puro Brca1 S1423A S1524A HA

   Plasmid

   #41968

   Depositor

   Stephen Elledge

   Article

   Cortez et al Science. 1999 Nov 5. 286(5442):1162-6.

   Insert

   BRCA1 (BRCA1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S1423A, S1524A

   Promoter

   Available since

   April 26, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pBABE-puro-Halo-hMRE11(H129N)

   Plasmid

   #208394

   Purpose

   To generate stable cell line expressing Halo-human MRE11(H129N) by Retroviral infection.

   Depositor

   Gaorav Gupta

   Article

   Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

   Insert

   Human MRE11 (MRE11 Human)
   

   Use

   Luciferase and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   H129N

   Promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-Flag-Polß(WT)-IRES-puro

    Plasmid

    #128653

    Purpose

    mammalian expression of Flag-Polß(WT) protein with puromycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Wild type

    Promoter

    CMV

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC118-mCtnnb1-FLIP-FlpE-Puro

    Plasmid

    #84541

    Purpose

    CRISPR - FLIP-FlpE targeting vector for mouse beta-catenin

    Depositor

    Bon-Kyoung Koo

    Article

    Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

    Insert

    mouse beta-catenin FLIP-FlpE - Puro targeting vector
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

12. XLone-Puro Cas13d-eGFP U6 SOX17 g2

    Plasmid

    #155188

    Purpose

    PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and SOX17 gRNA2

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Insert

    Cas13d SOX17 gRNA2
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC57-PB-PGK-tetR-KRAB-2A-Puro

    Plasmid

    #129536

    Purpose

    PiggyBac transposon carrying tetR-KRAB-2A-Puro under the PGK promoter.

    Depositor

    Hideyuki Okano

    Article

    Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

    Insert

    PB-PGK-tetR-KRAB-2A-Puro
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    hPGK promoter

    Available since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-PGK-RAP1A-E63-puro

    Plasmid

    #156167

    Purpose

    Lentiviral vector for expression of RAP1A-E63, with puromycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP1A (RAP1A Human)
    

    Use

    Lentiviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    changed Glutamine 63 to Glutamic acid

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBabe puro pI3K p110 CAAX

    Plasmid

    #13339

    Depositor

    Channing Der

    Article

    McFall et al Mol Cell Biol. 2001 Aug . 21(16):5488-99

    Insert

    pI3K p110 CAAX (PIK3CA Bovine)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pBabe-TetCMV-puro-mVenus-LOV

    Plasmid

    #22033

    Purpose

    Mammalian expression of mVenus tagged LOV2 domain

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mVenus-LOV
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Insert length based on Venus (720bp) + LOV (450bp)

    Promoter

    Available since

    Sept. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pGL3-U6-XsgRNA-ccdB-EF1a-Puromycin

    Plasmid

    #115519

    Purpose

    To construct multiple sgRNA expression vector for CRISPR

    Depositor

    Xingxu Huang

    Insert

    ccdB-sgRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBabe puro-HA-PIK3CA(H1047R;I848V)

    Plasmid

    #33253

    Depositor

    Thomas Roberts

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Retroviral

    Tags

    Hemagglutinin (HA)

    Expression

    Mammalian

    Mutation

    changed histidine 1047 to arginine and isoleucine…

    Promoter

    SV40

    Available since

    Jan. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pBabe puro H-Ras G12V Y40C

    Plasmid

    #12276

    Depositor

    Bob Weinberg

    Article

    Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

    Insert

    H-Ras G12V Y40C (HRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Contains G12V activating mutation. The Y40C mutat…

    Promoter

    Available since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pRetroX-TetOne-Puro-p62 deltaUBA

    Plasmid

    #204550

    Purpose

    Expresses p62 deltaUBA in a doxycycline-dependent manner in mammalian cells

    Depositor

    Masaaki Komatsu

    Article

    Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

    Insert

    p62 (SQSTM1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    deltaUBA

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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