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AV11_pDonor.EP.S1

Plasmid

#100296

Purpose

AAVS1-targeting expression plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

PuroR.T2A.EGFP expression unit

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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AV09_pDonor.EP.S1.TS

Plasmid

#100297

Purpose

AAVS1-targeting expression plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

PuroR.T2A.EGFP expression unit

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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AX53_pS.Donor.S1.TS

Plasmid

#100290

Purpose

AAVS1-targeting plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

18 bp RFLP (MluI+KspAI)

Use

CRISPR and Synthetic Biology; Human targeting

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pTS2163

Plasmid

#169632

Purpose

Tier-3 vector for CRISPR-mediated stable integration into the AAVS1 locus of the tetON-system (OTetO7-PCMVmin-SEAP-p2A-iRFP670-pA::PmPGK1-rtTA-pA::A3-pA)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

OTetO7-PCMVmin-SEAP-iRFP670::PmPGK1-rtTA-pA::A3

Expression

Mammalian

Promoter

tetO7-PhCMVmin / PPGK

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAB-NEO-PYL

Plasmid

#114393

Purpose

For PYL expression

Depositor

Vincent Dion

Article

Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

Insert

PYL

Tags

3xFLAG-SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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AX44_pS.Donor.S1

Plasmid

#100289

Purpose

AAVS1-targeting plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

18 bp RFLP (MluI+KspAI)

Use

CRISPR and Synthetic Biology; Human targeting

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pAB(EXPR-PYL-DNMT1-NEO)

Plasmid

#114396

Purpose

For PYL-DNMT1 expression

Depositor

Vincent Dion

Article

Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

Insert

PYL-DNMT1

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAB-HDAC5(mut-H-Y)

Plasmid

#114398

Purpose

For PYL-HDAC5 H1006Y expression

Depositor

Vincent Dion

Article

Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

Insert

PYL-HDAC5 H1006Y

Expression

Mammalian

Mutation

additional D593E compared to NCBI ref NP_005465.2

Promoter

CMV

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAB(EXPR-PYL-HDAC5-NEO)

Plasmid

#114395

Purpose

For PYL-HDAC5 expression

Depositor

Vincent Dion

Article

Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

Insert

PYL-HDAC5

Tags

3xFlag-NLS (internal)

Expression

Mammalian

Mutation

D593E compared with NCBI reference NP_005465.2

Promoter

CMV

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAB-HDAC5(mut-H-A)

Plasmid

#114397

Purpose

For PYL-HDAC5 H1006A expression

Depositor

Vincent Dion

Article

Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

Insert

PYL-HDAC5 H1006A

Tags

3xFlag-NLS (internal)

Expression

Mammalian

Mutation

D593E compared to NCBI ref NP_005465.2

Promoter

CMV

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAB-EXPR(PYL-cat_dom_HDAC5)

Plasmid

#114401

Purpose

For PYL-HDAC5 catalytic domain expression

Depositor

Vincent Dion

Article

Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

Insert

PYL-HDAC5 catalytic domain

Tags

3xFlag-NLS (internal)

Expression

Mammalian

Mutation

S220F and the deletion of M221

Promoter

CMV

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAB-EXPR(PYL-Nterm_dom_HDAC5)

Plasmid

#114402

Purpose

For PYL-HDAC5 N terminal domain expression

Depositor

Vincent Dion

Article

Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

Insert

PYL-HDAC5 N terminal domain

Tags

3xFlag-NLS (internal)

Expression

Mammalian

Mutation

D593E compared to NCBI ref NP_005465.2

Promoter

CMV

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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Hygro-Cas9 donor

Plasmid

#86883

Purpose

Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus with hygromycin selection

Depositor

Danwei Huangfu

Article

Shi et al Cell Stem Cell. 2017 Feb 8. pii: S1934-5

Insert

hSpCas9

Use

CRISPR

Tags

3xFlag, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Available Since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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pAB(EXPR-HDAC5-trun1)

Plasmid

#114400

Purpose

For PYL-HDAC5 aa 1-275 expression

Depositor

Vincent Dion

Article

Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

Insert

PYL-HDAC5 aa 1-275 (HDAC5 Human)

Tags

3xFlag-NLS (internal)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pC13P-iCLHN

Plasmid

#66580

Purpose

CLYBL safe harbor gene targeting donor expressing CAG-driven Nanoluc-HaloTag, compatible with pZT-C13 TALENs

Depositor

Jizhong Zou

Article

Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

Insert

Nanoluc-HaloTag

Expression

Mammalian

Promoter

CAG

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pAB(EXPR-PYL-HDAC3-NEO)

Plasmid

#114394

Purpose

For PYL-HDAC3 expression

Depositor

Vincent Dion

Article

Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

Insert

PYL-HDAC3 (HDAC3 Human)

Tags

3xFlag-NLS (internal)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-MYO7A-TAIL

Plasmid

#89585

Purpose

Expression plasmid for a positive control bait protein in NanoSPD 2.0 assays.

Depositor

Thomas Friedman

Article

Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

Insert

MYO7A (Myo7a Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Truncation encoding amino acids 870-2166 of MYO7A…

Promoter

CMV

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAB(EXPR-DS-HDAC5_HDAC3)

Plasmid

#114399

Purpose

For PYL-HDAC5 catalytic domain swap to HDAC3 catalytic domain expression

Depositor

Vincent Dion

Article

Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

Insert

PYL-HDAC5_HDAC3 catalytic domain swap (HDAC3, HDAC5 Human)

Tags

3xFlag-NLS (internal)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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Donor-attp-u6-blank-sgrna-backbone

Plasmid

#234076

Purpose

Blank donor for Perturb Seq landing pad with Pa01 recombinase at the AAVS1 loci

Depositor

Gary Hon

Article

Sivakumar et al bioRxiv [Preprint]. 2025 Jan 23:2025.01.

Insert

sgRNA-Blank

Use

Lentiviral

Expression

Mammalian

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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