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We narrowed to 7,666 results for: trac

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Showing: 6401 - 6450 of 7666 results

  1. pcDNA5-FRT-TO-Nsp8-HF_GC3opt

    Plasmid
    #157699
    Purpose
    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
    Tags
    His8-Flag
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Available Since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157699-plasmid-map-sequence-id-307863

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  3. pcDNA5-FRT-TO-Nsp7-Nsp8-HF_GC3opt

    Plasmid
    #157693
    Purpose
    mammalian expression of C-terminally His8-Flag tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    Nsp7-Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
    Tags
    His8-Flag
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Available Since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157693-plasmid-map-sequence-id-307835

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  10. pcs2-cDELE1(142-515)-sumo

    Plasmid
    #226107
    Purpose
    cDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) fused to sumo used for in-vitro translation by wheat germ extract in in-vitro ubiquitylation assays
    Depositor
    Michael Rape
    Article
    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:
    Insert
    DELE1 (DELE1 Human)
    Tags
    Sumo
    Expression
    Mammalian
    Mutation
    encodes only residues 142-515 of DELE1 (represent…
    Available Since
    Oct. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    226107-plasmid-map-sequence-id-449375

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  11. EF1a-HexaPro_Foldon-PDGFR-T2A-mCherry (pZYW079)

    Plasmid
    #194234
    Purpose
    Lentiviral expression vector expressing Jason McClellan lab's 6-proline Spike extracellular domain on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction marker
    Depositor
    Hana El-Samad
    Article
    Weinberg et al bioRxiv [Preprint]. 2021 Apr 20:2021.04.
    Insert
    EF1a-Spike ECD-PDGFR TMD-T2A-mCherry (S Synthetic)
    Use
    Lentiviral
    Promoter
    EF1a
    Available Since
    Sept. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    194234-plasmid-map-sequence-id-383907

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  12. AAV-TRE-dSaCas9-NLS-3xHA-3xSunTag_bGHpA

    Plasmid
    #177344
    Purpose
    AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence from doxycycine-inducible promoter
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    dead hSaCas9
    Use
    AAV and CRISPR
    Tags
    3x GCN4 peptide, 3xHA, and NLS
    Expression
    Mammalian
    Mutation
    D10A, N580A
    Promoter
    Tetracycline-dependent promoters
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177344-plasmid-map-sequence-id-353629

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  13. pLCHKOv3-intergenic-As

    Plasmid
    #209026
    Purpose
    Lentiviral vector expressing U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209026-plasmid-map-sequence-id-413692

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  14. pLCHKOv3-CD46 Ex3_1-Lb

    Plasmid
    #209027
    Purpose
    Lentiviral vector expressing U6 driven hybrid guide (hgRNA) targeting human CD46 Exon 3 for deletion using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    hgRNA for deletion of CD46 Exon 3 with SpCas9 tracrRNAv1 & LbCas12a DR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209027-plasmid-map-sequence-id-414806

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  15. pLCHKOv3-CD46 Ex3_2-Lb

    Plasmid
    #209028
    Purpose
    Lentiviral vector expressing U6 driven hybrid guide (hgRNA) targeting human CD46 Exon 3 for deletion using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    hgRNA for deletion of CD46 Exon 3 with SpCas9 tracrRNAv1 & LbCas12a DR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209028-plasmid-map-sequence-id-414792

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  16. pLCHKOv3-CD46 Ex3_3-Lb

    Plasmid
    #209029
    Purpose
    Lentiviral vector expressing U6 driven hybrid guide (hgRNA) targeting human CD46 Exon 3 for deletion using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    hgRNA for deletion of CD46 Exon 3 with SpCas9 tracrRNAv1 & LbCas12a DR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209029-plasmid-map-sequence-id-414782

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  17. pLCHKOv3-intergenic-Lb

    Plasmid
    #209030
    Purpose
    Lentiviral vector expressing U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases (CHyMErA system), respectively
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    intergenic hgRNA with SpCas9 tracrRNAv1 & LbCas12a DR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209030-plasmid-map-sequence-id-413690

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  18. pLCHKOv3-CD46 Ex3_1-As

    Plasmid
    #209031
    Purpose
    Lentiviral vector expressing U6 driven hybrid guide (hgRNA) targeting human CD46 Exon 3 for deletion using SpCas9 and AsCas12a nucleases (CHyMErA system)
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    hgRNA for deletion of CD46 Exon 3 with SpCas9 tracrRNAv1 & AsCas12a DR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209031-plasmid-map-sequence-id-414800

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  19. pLCHKOv3-CD46 Ex3_2-As

    Plasmid
    #209032
    Purpose
    Lentiviral vector expressing U6 driven hybrid guide (hgRNA) targeting human CD46 Exon 3 for deletion using SpCas9 and AsCas12a nucleases (CHyMErA system)
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    hgRNA for deletion of CD46 Exon 3 with SpCas9 tracrRNAv1 & AsCas12a DR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209032-plasmid-map-sequence-id-413524

    Enlarge

  20. pLCHKOv3-CD46 Ex3_3-As

    Plasmid
    #209033
    Purpose
    Lentiviral vector expressing U6 driven hybrid guide (hgRNA) targeting human CD46 Exon 3 for deletion using SpCas9 and AsCas12a nucleases (CHyMErA system)
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    hgRNA for deletion of CD46 Exon 3 with SpCas9 tracrRNAv1 & AsCas12a DR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209033-plasmid-map-sequence-id-413572

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  22. pUt-mNF-GFP

    Plasmid
    #199220
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with GFP Negative-Feedback circuit (mNF-GFP). Use BlastR for positive selection and HSV-TK for negative selection. 
    Depositor
    Gabor Balazsi
    Article
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Insert
    hTetR::P2A::EGFP
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV D2ir promoter
    Available Since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199220-plasmid-map-sequence-id-396466

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Showing: 6401 - 6450 of 7666 results