We narrowed to 8,809 results for: CAG

1. CAG-loxP-3xpA-H2BsfGFP-tdTomato - long GSA linker E

   Plasmid

   #113845

   Purpose

   Histone H2B-superfolder GFP and tdTomato linked by long GSA linker

   Depositor

   Mario Capecchi

   Insert

   H2B-sfGFP-GSA_linker-tdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP-CAG-Ace2N-4AA-mNeon A122D-WPRE-bGH-polyA

   Plasmid

   #172911

   Purpose

   Mammalian expression of voltage sensor

   Depositor

   GENIE Project

   Article

   Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

   Insert

   Ace2N-4AA-mNeon A122D
   

   Use

   Tags

   soma localization targeting sequence

   Expression

   Mammalian

   Mutation

   A122D

   Promoter

   CAG

   Available since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)

   Plasmid

   #107941

   Purpose

   Mammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutions

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized enAsCas12a (E174R/S542R/K548R)
   

   Use

   Tags

   3x HA and NLS (nucleoplasmin)

   Expression

   Mammalian

   Mutation

   E174R, S542R and K548R

   Promoter

   CAG

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAVS1-Puro-CAG-fl-STOP-fl-ZEB2-GFP-Flag-WPRE-poly(A)

   Plasmid

   #165456

   Purpose

   Inducible ZEB2 expression from AAVS1 locus

   Depositor

   Madeline Lancaster

   Article

   Benito-Kwiecinski et al Cell. 2021 Apr 15;184(8):2084-2102.e19.

   Insert

   ZEB2 (ZEB2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLQ2814 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SpdCas9

   Plasmid

   #84247

   Purpose

   Expresses direct fusion VPR-Sp dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   VPR-tagBFP-Sp dCas9
   Puro
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   2xNLS (SV40), HA tag, VPR, and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and PGK

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMB952:PB-CAG-PuroR-nGFP-FLEx-SaCas9-P2A-mCherry-WPRE

   Plasmid

   #168107

   Purpose

   PiggyBac transposon vector constitutively driving PuroR-eGFP and conditionally expressing S.aureus Cas9 and mCherry

   Depositor

   Erich Jarvis

   Article

   Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

   Insert

   Cas9-P2A-mCherry
   

   Use

   CRISPR and Cre/Lox

   Tags

   HA

   Expression

   Mutation

   Promoter

   CAG

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-SpdCas9

   Plasmid

   #84245

   Purpose

   Expresses direct fusion KRAB-Sp dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   KRAB-tagBFP-Sp dCas9
   Puro
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   2xNLS (SV40), HA tag, KRAB, and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and PGK

   Available since

   Nov. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. YET1000: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X4)

   Plasmid

   #104571

   Purpose

   Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to four DmrA domains

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

   Insert

   human codon optimized ‘dead’ Cpf1 fused to four DmrA domains
   

   Use

   CRISPR

   Tags

   NLS-3xHA-3xFLAG-4xDmrA

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. PP023 - CAG::QuasAr6a-TS-dmCitrine-TSx3-ER2-p2a- jRGECO1a-CAAX

   Plasmid

   #182063

   Purpose

   Co-expression of Arch-based voltage indicator (QuasAr6a) and membrane-localized calcium reporter (jRGECO1a-CAAX)

   Depositor

   Adam Cohen

   Article

   Landau et al eLife. 2022 Mar 23;11. pii: 76993. doi:

   Insert

   QuasAr6a-TS-dmCitrine-TSx3-ER2-p2a- jRGECO1a-CAAX
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   March 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SadCas9

    Plasmid

    #84246

    Purpose

    Expresses direct fusion VPR-Sa dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    VPR-tagBFP-Sa dCas9
    Puro
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    Nucleoplasmin NLS, SV40 NLS, VPR, and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and PGK

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pK263.CAG-loxP-stop-loxP-NLSturboRFP-ires-tTA-WPRE

    Plasmid

    #89587

    Purpose

    For nucleus-specific turboRFP labeling in single cells, pK263 should be used with pK031.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    nuclear localization signal (nls) turboRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-lox-rox-STOP-rox-lynScarlet-lox-Lyn-NeonGreen

    Plasmid

    #175259

    Purpose

    dual plasmid labelling membrane target protein of different neuronal population with different flurophore

    Depositor

    Sebastián Dupraz, Frank Bradke

    Article

    Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

    Insert

    Lox-rox-STOP-rox-lyn-mScarlet-pA-lox
    

    Use

    Cre/Lox; Dre/rox

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    pCAG

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGP-CAG-Ace2N-4AA-mNeon-ST A122D-WPRE-bGH-polyA

    Plasmid

    #172912

    Purpose

    Mammalian expression of voltage sensor; soma localization targeting sequence

    Depositor

    GENIE Project

    Article

    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

    Insert

    Ace2N-4AA-mNeon-ST A122D
    

    Use

    Tags

    soma localization targeting sequence

    Expression

    Mammalian

    Mutation

    A122D

    Promoter

    CAG

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. JG674: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X1)

    Plasmid

    #104568

    Purpose

    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to one DmrA domain

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    dLbCpf1(D832A)-DmrA(x1)
    

    Use

    Tags

    3x FLAG, 3x HA, and NLS

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. JG676: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X2)

    Plasmid

    #104569

    Purpose

    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to two DmrA domains

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    dLbCpf1(D832A)-DmrA(x2)
    

    Use

    Tags

    3x FLAG, 3x HA, and NLS

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMB1052:miniTol2-U6-(empty)SaCas9_gRNA-CAG-Cre-P2A-NeoR-WPRE

    Plasmid

    #168108

    Purpose

    Tol2 transposon vector of empty U6-S.aureus Cas9 gRNA cassette and CAG-driven Cre-NeoR

    Depositor

    Erich Jarvis

    Article

    Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-CAG-NOTCH2NL-delta C terminus (N2NL-delta C)-ires-mCherry

    Plasmid

    #122959

    Purpose

    Lentiviral expression of NOTCH2NL delta C terminus under CAG promoter with mCherry expression

    Depositor

    Pierre Vanderhaeghen

    Article

    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

    Insert

    NOTCH2NL-delta C terminus (NOTCH2NLB Human)
    

    Use

    Lentiviral

    Tags

    6xmyc tag

    Expression

    Mutation

    deletion of C terminus from full length NOTCH2NLB

    Promoter

    CAG promoter

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-CAG-NOTCH2NL-delta C terminus (N2NL-delta C)-ires-EGFP

    Plasmid

    #122956

    Purpose

    Lentiviral expression of NOTCH2NL delta C terminus under CAG promoter with EGFP expression

    Depositor

    Pierre Vanderhaeghen

    Article

    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

    Insert

    NOTCH2NL-delta C terminus (NOTCH2NLB Human)
    

    Use

    Lentiviral

    Tags

    6xhis tag

    Expression

    Mutation

    deletion of C terminus from full length NOTCH2NLB

    Promoter

    CAG promoter

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-pCAG-FRT(F3F5)-ChRmine3.3-p2A-oScarlet,rev.-WPRE

    Plasmid

    #234895

    Purpose

    CAG-driven, Flp-dependent ChRmine for optogenetic excitation of neurons with an oScarlet tag

    Depositor

    Silvia Arber

    Article

    Falasconi et al Nature. 2025 May 28. doi: 10.1038/s41586

    Insert

    FRT(F3F5)-ChRmine3.3-p2A-oScarlet
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-eCas9-T2A-EGFP-U6-gRNA targeting Nrl-no ITR and f1 ori

    Plasmid

    #234737

    Purpose

    All-in-one CRISPR/Cas9 vector encodes high-fidelity eSpCas9 and a gRNA targeting Nrl, efficiently reprogramming rod precursors into cone-like cells in the mouse retina.

    Depositor

    Kirill Martemyanov

    Article

    Zhao et al Nat Commun. 2025 Apr 22;16(1):3768. doi:

    Insert

    Nrl (Nrl Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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