We narrowed to 10,316 results for: plasmids 101

1. pAAV-hSyn-mCherry.3xFLAG.NOS1AP-S-WPRE

   Plasmid

   #127867

   Purpose

   pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP-S fusion protein under the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

   Insert

   Nos1ap (Nos1ap Human, Mouse)
   

   Use

   AAV

   Tags

   3xFLAG and mCherry

   Expression

   Mutation

   constructed by blunt end fusion of the 5’ 51 nucl…

   Promoter

   hSyn

   Available Since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-500-WPRE

   Plasmid

   #174134

   Purpose

   pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-500 fusion protein under the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Freudenberg et al EBioMedicine. 2021 Aug 26;71:103565. doi

   Insert

   Nos1ap (Nos1ap Mouse)
   

   Use

   AAV

   Tags

   3xFLAG and mCherry

   Expression

   Mutation

   Only contains the sequences coding for amino acid…

   Promoter

   hSyn

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EF1α-Flex/3'USS-hM4D/2A/GFP(ATG mut)

   Plasmid

   #197892

   Purpose

   Can be used to generate AAV virus that will express hM4D/2A/GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   hM4D/2A/GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   N/A

   Promoter

   EF1a

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIS1 ATP2B1

   Plasmid

   #60788

   Purpose

   Luciferase reporter to measure miR-155 mediated differential repression. Contains ATP2B1 3' UTR and wild-type miR-155 sites

   Depositor

   David Bartel

   Article

   Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi

   Insert

   ATP2B1 3'UTR and wild-type miR-155 binding site (ATP2B1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   thymidine kinase (HSV-TK promoter)

   Available Since

   Oct. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. R619-M68-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-529)-3C-His8-SBP

   Plasmid

   #166019

   Purpose

   mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD) optimized for serology

   Depositor

   Dominic Esposito

   Article

   Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

   Insert

   SARS-CoV-2 RBD (S )
   

   Use

   Tags

   3C-His8-SBP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV51

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIS1 LMBRD2

   Plasmid

   #60800

   Purpose

   Luciferase reporter to measure miR-155 mediated differential repression. Contains LMBRD2 3' UTR and wild-type miR-155 sites

   Depositor

   David Bartel

   Article

   Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi

   Insert

   LMBRD2 3'UTR and wild-type miR-155 binding site (LMBRD2 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   thymidine kinase (HSV-TK promoter)

   Available Since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. human ETV6 gRNA-4

   Plasmid

   #133404

   Purpose

   human ETV6 gRNA-3 and 4 is a pair of gRNAs. Four ETV6 gRNA plasmids used the same backbone(Addgene plasmid#41824). ETV6 gRNA-4 target the second ETV6 exon.

   Depositor

   Linzhao Cheng

   Article

   Wu et al Stem Cell Res. 2018 May;29:6-14. doi: 10

   Insert

   ETV6 (ETV6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOGS539_GLuc

   Plasmid

   #226718

   Purpose

   Plasmid enabling yeast-mediated expression and secretion of Gaussia luciferase (GLuc)

   Depositor

   Gautam Dantas

   Article

   Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

   Insert

   Gaussia Luciferase
   

   Use

   Tags

   HA tag and Mating factor alpha secretion signal

   Expression

   Yeast

   Mutation

   Promoter

   pTEF1

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3-noSV40-humanEC1.45_min1-2_4XEboxMutant

   Plasmid

   #215026

   Purpose

   Firefly luciferase enhancer reporter plasmid with minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with E-boxes mutated

   Depositor

   Joanna Wysocka

   Article

   Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

   Insert

   Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with E-boxes mutated
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   4X E-box mutations within Coordinator motifs

   Promoter

   SV40

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-503-WPRE

    Plasmid

    #174133

    Purpose

    pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-503 fusion protein under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Freudenberg et al EBioMedicine. 2021 Aug 26;71:103565. doi

    Insert

    Nos1ap (Nos1ap Mouse)
    

    Use

    AAV

    Tags

    3xFLAG and mCherry

    Expression

    Mutation

    Only contains the sequences coding for amino acid…

    Promoter

    hSyn

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-427-WPRE

    Plasmid

    #174135

    Purpose

    pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-427 fusion protein under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Freudenberg et al EBioMedicine. 2021 Aug 26;71:103565. doi

    Insert

    Nos1ap (Nos1ap Mouse)
    

    Use

    AAV

    Tags

    3xFLAG and mCherry

    Expression

    Mutation

    Only contains the sequences coding for amino acid…

    Promoter

    hSyn

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. R619-M96-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(318-529)-3C-His8-SBP

    Plasmid

    #166020

    Purpose

    mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD) optimized for serology

    Depositor

    Dominic Esposito

    Article

    Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

    Insert

    SARS-CoV-2 RBD (S )
    

    Use

    Tags

    3C-His8-SBP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV51

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFnCpf1GG

    Plasmid

    #131010

    Purpose

    Backbone plasmid for generating CRISPR arrays for FnCas12a. Contains a GFP-dropout cassette and a direct repeat.

    Depositor

    Chase Beisel

    Article

    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

    Inserts

    direct repeat of FnCas12a
    GFP expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pK.myc-MYPT1

    Plasmid

    #24101

    Depositor

    Masumi Eto

    Article

    Eto et al Cell Motil Cytoskeleton. 2005 Oct . 62(2

    Insert

    Myosin-targeting subunit-1 (PPP1R12A Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    S50G, P415Q

    Promoter

    Available Since

    March 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. PLKO.1-G3BP1-CDS

    Plasmid

    #136039

    Purpose

    G3BP1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (CGGGAATTTGTGAGACAGTAT)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCerulean-Keratin-17

    Plasmid

    #55379

    Purpose

    Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Keratin (KRT18 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    V282L

    Promoter

    CMV

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAsCpf1GG

    Plasmid

    #131011

    Purpose

    Backbone plasmid for generating CRISPR arrays for AsCas12a. Contains a GFP-dropout cassette and a direct repeat.

    Depositor

    Chase Beisel

    Article

    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

    Inserts

    direct repeat of AsCas12a
    GFP expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHW1176

    Plasmid

    #229871

    Purpose

    gene trap RMCE plasmid with GFP-C1-tbb-2 3'UTR::SEC for RMCE cloned in the destination vector pHW1133 to swap driver via RMCE

    Depositor

    Han Wang

    Article

    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

    Insert

    GFP-C1-tbb-2 3'UTR::SEC
    

    Use

    Tags

    GFP-C

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    June 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHW1643

    Plasmid

    #229876

    Purpose

    gene trap RMCE plasmid with Tet-On[rTetR-linker-QFAD]-act-4 3' UTR::SEC cloned in the destination vector pHW1133 to swap driver via RMCE

    Depositor

    Han Wang

    Article

    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

    Insert

    Tet-On[rTetR-linker-QFAD]-act-4 3' UTR::SEC
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHW1177

    Plasmid

    #229872

    Purpose

    gene trap RMCE plasmid with Tet-Off[TetR-linker-QFAD]-act-4 3'UTR::SEC cloned in the destination vector pHW1133 to swap driver via RMCE

    Depositor

    Han Wang

    Article

    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

    Insert

    Tet-Off[TetR-QFAD]-act-4 3'UTR::SEC
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge