We narrowed to 8,860 results for: sgRNA

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pAT-9208

Plasmid

#124221

Purpose

Cloning plasmid for a self-targeting gRNA library

Depositor

Ervin Welker

Article

Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

Insert

self-targeting gRNA

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pDIV494

Plasmid

#177701

Purpose

Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

Depositor

Uffe Mortensen

Article

Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

Inserts

Cas9
Nat
sgRNA Targeting ADE2 locus in D.hansenii: AGCTAAGCAGATTAATGCAT

Use

CRISPR

Tags

SV40

Expression

Bacterial and Yeast

Promoter

RNR2p (Debaryomyces hansenii ), SNR52p (Candida s…

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)_No_FLAG_ATP1A1-G7

Plasmid

#173203

Purpose

Expresses the ATP1A1 G7 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 17. pX330-like plasmid.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G7 sgRNA + FLAGless enhanced specificity Cas9 (1.1)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQDKN0761

Plasmid

#185630

Purpose

Modified tobacco rattle virus RNA2 vector expressing an sgRNA fused to trucated flowering locus T which targets phytoene desaturase (PDS) of Nicotiana benthamiana, functionally equivalent to pEE393

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

sgRNA

Use

CRISPR and Synthetic Biology

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Aa3.8

Plasmid

#136376

Purpose

Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 is used

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti CRISPR V2 sgMTFMT_1

Plasmid

#106318

Purpose

Express Cas9 and sgRNA targeting MTFMT

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting MTFMT

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pgRNA_hMECP2_promoter_No4

Plasmid

#194899

Purpose

Lentiviral construct with mCherry and Puro cassettes to express sgRNA-4 targeting the promoter of human MECP2

Depositor

Shawn Liu

Article

Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

Insert

sgRNA targeting human MECP2 promoter No.4

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pUC CBA-SpCas9N863Anickase.EF1a-BFP.sgLMNApair1

Plasmid

#98976

Purpose

Cas9 Homologous Recombination Reporter. SpCas9N863A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 44bp 3' overhangs. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNAs pair 1 and Cas9(N863A)

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pSECB sgMTHFD2L_1

Plasmid

#106306

Purpose

Express Cas9 and sgRNA targeting MTHFD2L

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting MTHFD2L (MTHFD2L Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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pSECB sgMTHFD2L_2

Plasmid

#106307

Purpose

Express Cas9 and sgRNA targeting MTHFD2L

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting MTHFD2L (MTHFD2L Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti CRISPR sgSHMT1_2

Plasmid

#106312

Purpose

Express Cas9 and sgRNA targeting SHMT1

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting SHMT1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAT-9222

Plasmid

#124222

Purpose

Cloning plasmid for EGxFP assay with self-targeting gRNA-s

Depositor

Ervin Welker

Article

Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

Insert

self-targeting gRNA between EGFP halves

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti CRISPR V2 sgShmt2_2

Plasmid

#106315

Purpose

Express Cas9 and sgRNA targeting mShmt2

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting mShmt2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti CRISPR sgSHMT2_2

Plasmid

#106310

Purpose

Express Cas9 and sgRNA targeting SHMT2

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting SHMT2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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APq5210

Plasmid

#66086

Purpose

co-expression of Cas9 and a sgRNA targeting K08F4.2 in the middle of the ORF

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APa4-2

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
pV1210

Plasmid

#242891

Purpose

Modified plasmid from pV1200 to perform ENO1-SI CRISPR in Candidozyma auris

Depositor

Patrick Van Dijck

Article

Sofras et al bioRxiv [Preprint]. 2025 Jan 11:2025.01.

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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MIGR1-U6gRNA2-Filler

Plasmid

#237401

Purpose

For the sequential insertion of two guide RNAs into two U6gRNA cassettes.

Depositor

Zuoming Sun

Article

Zhong et al STAR Protoc. 2025 Jun 20;6(2):103831. do

Inserts

U6gRNA-1
U6gRNA-2
PGK-TagBFP

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

PGK promoter, U6 promoter, and U6, PGK

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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pQdCas12a.luxR(mut)-sggfp(B)

Plasmid

#236041

Purpose

The plasmid pQdCas12a.luxR(mut)-sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.

Depositor

Radhakrishnan Mahadevan

Article

Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-

Insert

quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene

Use

CRISPR

Promoter

quorum sensing promoter

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-Cas9-NS-A

Plasmid

#207823

Purpose

Dual expression of Cas9 and non-specific sgRNA

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

non-specific sgRNA

Use

CRISPR and Lentiviral

Tags

FLAG

Promoter

EF-1a; U6

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-Cas9-NS-B

Plasmid

#207824

Purpose

Dual expression of Cas9 and non-specific sgRNA

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

non-specific sgRNA

Use

CRISPR and Lentiviral

Tags

FLAG

Promoter

EF-1a; U6

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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