We narrowed to 1,305 results for: pET28a
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Plasmid#172096PurposeExpresses a fusion protein of Gluc and iLID, which dimerizes with a sspB-tagged protein when the bioluminescent reaction is initiated, in bacteriaDepositorInsertGaussia Luciferase - iLID
UseTagsExpressionBacterialMutationGluc Mutations - M43L, M110LPromoterT7Available sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HsSENP3(307-574)
Plasmid#71481PurposeEncodes the catalytic domain of human SENP3. Suitable for in vitro transcription/translation (T7).DepositorInsertSUMO1/sentrin specific peptidase 3 (SENP3 Human)
UseTagsHexahistidineExpressionBacterialMutationDeleted amino acids 1-306 (catalytic domain only)PromoterT7Available sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-hDcp2 E147Q, E148Q
Plasmid#72215Purposebacterial expression of His-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)DepositorInsertDcp2 (DCP2 Human)
UseTagsHisExpressionBacterialMutationE147Q, E148QPromoterT7Available sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 aa 1-160-GCN4
Plasmid#74608PurposeExpresses artificially dimerized Hook domain of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-160 (HOOK3 Human)
UseTags6x His, GCN4, Strep II, and superfolder GFPExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
WT N-His Ana GvpC in pET28a
Plasmid#153294PurposeExpresses N-terminal His-tagged GvpC from Anabaena flos-aquae in E.coliDepositorInsertGas vesicle protein C (N-terminal his-tagged)
UseTagsHis-tagExpressionBacterialMutationAdded a hexahistidine tag after the methionine at…PromoterT7Available sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pF714-pET28a-Hs-FL-METTL20-NHis wt
Plasmid#85115Purposeexpression of recombinant human full-length METTL20 wt with N-terminal 6xHis-tagDepositorInserthuman full-length METTL20 (METTL20 Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) 6xHis-TEV-IFIT5
Plasmid#53560Purposebacterial expression of IFIT5DepositorInsertIFIT5 (IFIT5 Human)
UseTags6xHIS and TEVExpressionBacterialMutationPromoterT7Available sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hisx6-Sirt6-H133Y
Plasmid#20279DepositorInsertSirt6 (Sirt6 Mouse)
UseTagsHisx6ExpressionBacterialMutationHistidine 133 to TyrosinePromoterAvailable sinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Gluc-EL222-his
Plasmid#172097PurposeExpresses the fusion protein of Gluc and the light-activated transcription factor EL222 in bacteriaDepositorInsertGaussia Luciferase - EL222
UseTagsExpressionBacterialMutationGluc mutations - M43L, M110LPromoterT7Available sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MBP-(CBX1)3-eReader
Plasmid#135090PurposeBacterial expression of engineered CBX1 fusions with enhanced binding to H3K9meDepositorInsertCBX1 chromobox protein 1, engineered x3
UseTags6xhis-tag and MBPExpressionBacterialMutationlysine 43 mutated to alanine; aspartic acid 59 mu…PromoterT7Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TAF4(827-909)
Plasmid#105620Purposebacterially express TAF4 HFDDepositorInsertTAF4-HFD(827-909) (Taf4 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28A-p53 (1-73) (P12A P13A)
Plasmid#62066PurposeExpression of human p53 (residues 1-73) (P12A, P13A) in e. coliDepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationContains TAD residues 1-73; P12A, P13APromoterT7Available sinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hisx6-Sirt6-S56A
Plasmid#20278DepositorInsertSirt6 (Sirt6 Mouse)
UseTagsHisx6ExpressionBacterialMutationSerine 56 to AlaninePromoterAvailable sinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28a-hfYFP-TEV-Bcx
Plasmid#191195PurposeFusion of hfYFP and Bacillus circulans xylanase separated by a TEV cleavage site for fluorescence-assisted protein purificationDepositorInserthfYFP & Bcx fusion
UseTags6xHis and Bacillus circulans xylanaseExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-hfYFP-TEV-mScarlet-i
Plasmid#191196PurposeFusion of hfYFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purificationDepositorInserthfYFP & mScarlet-I fusion
UseTags6xHis and mScarlet-IExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-hfYFP-TEV-SAV
Plasmid#191197PurposeFusion of hfYFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purificationDepositorInserthfYFP & Streptavidin fusion
UseTags6xHis and Streptavidin core domain, amino acids 1…ExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-LSSmGFP-TEV-mScarlet-i
Plasmid#191199PurposeFusion of LSSmGFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purificationDepositorInsertLSSmGFP & mScarlet-I fusion
UseTags6xHis and mScarlet-IExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-LSSmGFP-TEV-SAV
Plasmid#191200PurposeFusion of LSSmGFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purificationDepositorInsertLSSmGFP & Streptavidin fusion
UseTags6xHis and Streptavidin core domain, amino acids 1…ExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-NEDD4L_W2-W3-W4-HECT (381)
Plasmid#187708PurposeExpress his-tagged NEDD4L W2-W3-W4-HECT in bacterial cellsDepositorInsertNEDD4L residues 381-975 (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-380PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-NEDD4L_W3-W4-HECT (492)
Plasmid#187709PurposeExpress his-tagged NEDD4L W3-W4-HECT in bacterial cellsDepositorInsertNEDD4L residues 492-975 (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-491PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FKBP-IRSp53(pLxIS)
Plasmid#221336PurposeOverexpression of IRSp53 pLxIS domain in bacteriaDepositorInsertIRSp53 pLxIS domain (Baiap2 Mouse)
UseTags6xHis-4xFKBPExpressionBacterialMutationPromoterT7Available sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FKBP-MAVS(pLxIS)
Plasmid#221335PurposeOverexpression of MAVS pLxIS domain in bacteriaDepositorInsertMAVS pLxIS domain (Mavs Mouse)
UseTags6xHis-4xFKBPExpressionBacterialMutationPromoterT7Available sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FKBP-TRIF(Nterm)
Plasmid#221334PurposeOverexpression of TRIF Nterm domain in bacteriaDepositorInsertTRIF Nterm domain (Ticam1 Mouse)
UseTags6xHis-4xFKBPExpressionBacterialMutationPromoterT7Available sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FKBP-GMIP(pLxIS)
Plasmid#221337PurposeOverexpression of GMIP pLxIS domain in bacteriaDepositorInsertGMIP pLxIS domain (Gmip Mouse)
UseTags6xHis-4xFKBPExpressionBacterialMutationPromoterT7Available sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SnoopTag-SpyTag-(AffiHER2)x3
Plasmid#216281PurposeThree affibodies to HER2 linked in a chain for bacterial expression. SnoopTag and SpyTag allow isopeptide bond formation to their cognate CatcherDepositorInsertSnoopTag-SpyTag-(AffiHER2)x3
UseAffinity Reagent/ AntibodyTagsHis6, SnoopTag, and SpyTagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a LZ LED 4CA-FLAG
Plasmid#199681PurposeBacterial expression of Leucine Zipper (LZ) LYCHOS LED 4CA-FlagDepositorInsertLeucine zipper LYCHOS LED 4CA (GPR155 Human)
UseTags6xHis and FlagExpressionBacterialMutationC595.604.629.638 of LYCHOS mutated to APromoterT7 promoterAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a LZ LED Y551A-FLAG
Plasmid#199680PurposeBacterial expression of Leucine Zipper (LZ) LYCHOS LED Y551A-FlagDepositorInsertLeucine zipper LYCHOS LED Y551A (GPR155 Human)
UseTags6xHis and FlagExpressionBacterialMutationY551 of LYCHOS mutated to APromoterT7 promoterAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a Cdk2ap1ΔN (MT2B2)-MutTER
Plasmid#178035PurposeExpression vector for the purpose of protein purification.DepositorInsertCdk2ap1 (Cdk2ap1 Mouse)
UseNonviralTags6xHIS - Thrombin - MBP- TEV-TRSExpressionBacterialMutationFirst N-Terminal 27aa are deleted and Changed T(8…PromoterT7LacIAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
PET28a-cpLumiLuc-amylase sensor
Plasmid#186293PurposeExpression alpha-amylase sensor in e coliDepositorInsertcpLumiLuc-amylase sensor
UseTagsHisTagExpressionBacterialMutationPromoterAvailable sinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
PET28a-cpLumiLuc-HSA sensor
Plasmid#186294PurposeExpression Human serum albumin sensor in e coliDepositorInsertcpLumiLuc-HSA sensor
UseTagsHisTagExpressionBacterialMutationPromoterAvailable sinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (E60amb)-His
Plasmid#168043PurposeExpresses anticalin targeting CTLA-4 with the codon encoding E60 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationGlu 60 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (E143amb)-His
Plasmid#168046PurposeExpresses anticalin targeting CTLA-4 with the codon encoding E143 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationGlu 143 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (S87amb)-His
Plasmid#168044PurposeExpresses anticalin targeting CTLA-4 with the codon encoding S87 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationSer 87 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (N116amb)-His
Plasmid#168045PurposeExpresses anticalin targeting CTLA-4 with the codon encoding N116 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationAsn 116 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (N21amb)-His
Plasmid#168041PurposeExpresses anticalin targeting CTLA-4 with the codon encoding N21 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationAsn 21 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (I55amb)-His
Plasmid#168042PurposeExpresses anticalin targeting CTLA-4 with the codon encoding I55 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationIle 55 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(delta C-term)
Plasmid#112731PurposeThis mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.DepositorInsert6His-TEV-TBP6.7(delta C-term)
UseTags6His-TEVExpressionBacterialMutationdelta C-termPromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 D102A, E108A
Plasmid#74618PurposeExpresses a double mutant truncation of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-239, D102A, E108A (HOOK3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationD102A, E108APromoterT7Available sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG_N2N3-B1-B2-HIS-ybbr
Plasmid#117979PurposeSdrG N2 and N3, B1 and B2 domains with C-terminal ybbr tag for covalent immobilizationDepositorInsertSdrG_N2N3-B1-B2
UseTagsH3V 3C, HIS, and ybbrExpressionBacterialMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG_N2N3-B1-HIS-ybbr
Plasmid#117980PurposeSdrG N2 and N3, B1 domains with C-terminal ybbr tag for covalent immobilizationDepositorInsertSdrG_N2N3-B1
UseTagsH3V 3C, HIS, and ybbrExpressionBacterialMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only