Addgene

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pSIL-eGFP

Plasmid

#52675

Purpose

shRNA silencing plasmid with eGFP transfection marker

Depositor

Johannes Hell

Article

Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

Type

Empty backbone

Use

RNAi

Tags

CMV-EGFP

Expression

Mutation

Promoter

CMV and U6

Availability

Academic Institutions and Nonprofits only

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pCMV-Kl-EGFP

Plasmid

#45532

Depositor

Zipora Yablonka-Reuveni

Article

Phelps et al FEBS J. 2016 May;283(9):1653-68. doi: 10

Insert

alpha Klotho (Kl Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pEGFP-PH1(WT)

Plasmid

#86599

Purpose

Expresses eGFP-tagged PH(WT) domain of human Akt1

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 PH domain (AKT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt1(WT)

Plasmid

#86637

Purpose

Expresses eGFP-tagged human Akt1(WT)

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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EGFP-Myo1b

Plasmid

#187362

Purpose

Expresses rat myosin 1b isoform b labelled with EGFP

Depositor

Evelyne Coudrier

Article

Raposo et al Mol Biol Cell. 1999 May . 10(5):1477-94.

Insert

myosin 1b isoform b (Myo1b Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AKT-PH-GFP

Plasmid

#18836

Depositor

Craig Montell

Article

Kwon et al Mol Cell. 2007 Feb 23. 25(4):491-503.

Insert

AKT-PH domain (AKT1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

contains amino acids 1-148 (compared to GenBank r…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCMV-pOTC-GFP

Plasmid

#205724

Purpose

Expression of GFP fused with pOTC, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

pOTC-GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_CSF2RA_CRLF2

Plasmid

#205798

Purpose

Express mEGFP-tagged fusion protein, CSF2RA_CRLF2 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

CSF2RA_CRLF2 (CSF2RA, CRLF2 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pBz172_CsoS2_N1_GFP

Plasmid

#140852

Purpose

His-tagged first N-peptide of NTD fused to Superfolder GFP ([N1]-GFP) used for BLI and MST, pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 NTD repeat 1 fused to GFP

Use

Tags

Hexahistidine tag, Strep-Tag II, and Superfolder …

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pBz173_CsoS2_N2_GFP

Plasmid

#140853

Purpose

His-tagged second N-peptide of NTD fused to Superfolder GFP ([N2]-GFP) used for BLI, pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 NTD repeat 2 fused to GFP

Use

Tags

Hexahistidine tag, Strep-Tag II, and Superfolder …

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pBz174_CsoS2_N3_GFP

Plasmid

#140854

Purpose

His-tagged third N-peptide of NTD fused to Superfolder GFP ([N3]-GFP) used for BLI, pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 NTD repeat 3 fused to GFP

Use

Tags

Hexahistidine tag, Strep-Tag II, and Superfolder …

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pBz175_CsoS2_N4_GFP

Plasmid

#140855

Purpose

His-tagged fourth N-peptide of NTD fused to Superfolder GFP ([N4]-GFP) used for BLI, pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 NTD repeat 4 fused to GFP

Use

Tags

Hexahistidine tag, Strep-Tag II, and Superfolder …

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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IL2Rα-EGFP

Plasmid

#86055

Purpose

A mammalian expression plasmid encoding IL2Rα (interleukin-2 receptor subunit alpha) with C-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Interleukin-2 receptor subunit alpha (IL2RA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcry:mCherry,-600unc:cct3GFP

Plasmid

#172528

Purpose

alphaA-crystallin promoter (cry) drives mCherry expression in the lens. The 600bp unc-45b muscle-specific promoter drives expression of GFP-tagged zebrafish cct3.

Depositor

Joachim Berger

Article

Berger et al Cell Rep. 2018 Jan 9;22(2):313-322. doi:

Insert

cct3-GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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APEX2 - Tubulin in pEGFP

Plasmid

#66171

Purpose

APEX2 fused to the N-terminus of alpha tubulin

Depositor

Alice Ting

Article

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

APEX2 - alpha tubulin

Use

Tags

Flag epitope tag (DYKDDDDK)

Expression

Mammalian

Mutation

APEX2 contains 4 mutations relative to wt soybean…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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L304-EGFP-Tubulin-WT

Plasmid

#64060

Purpose

EGFP fused at the N-terminus of tubulin; lentiviral construct

Depositor

Weiping Han

Article

Yang et al Biochem J. 2013 Feb 1;449(3):605-12. doi

Insert

tubulin

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCMV-pOTC-3H-GFP

Plasmid

#205725

Purpose

Expression of GFP fused with pOTC-3H, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase and a thermostable three helix-bundle

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

pOTC-3H-GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pUAST ɑSynWT-EGFP

Plasmid

#201249

Purpose

allows the expression of the insert in Drosophila melanogaster

Depositor

Pedro Domingos

Article

Pocas et al Hum Mol Genet. 2015 Apr 1;24(7):1898-907

Insert

ɑ-Synuclein (SNCA Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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NSS-GFP

Plasmid

#163661

Purpose

Expresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from TNF and TMD and luminal domain from ST6GAL1 in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and tumor necrosis factor (ST6GAL1, TNF Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

This chimera contains the cytosolic tail from TNF…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
SNS-GFP

Plasmid

#163662

Purpose

Expresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from ST6GAL1, TMD from TNF and luminal domain from ST6GAL1 in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and tumor necrosis factor (ST6GAL1, TNF Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

This chimera contains the cytosolic tail from ST,…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge