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pHAGE2-TetOminiCMV-OSM

Plasmid

#136555

Purpose

Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, cMyc

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Oct4-P2A-Sox2-E2A-cMyc (Myc, Pou5f1, Sox2 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial

Mutation

Promoter

tetO-miniCMV (dox-inducible)

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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KHBD00401

Plasmid

#34511

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG1070 (Alh Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-Casp3 C163A-myc

Plasmid

#11814

Depositor

Guy Salvesen

Article

Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

Insert

Caspase 3 catalytic mutant (CASP3 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

C163A

Promoter

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

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pUC6

Plasmid

#49793

Purpose

E. coli cloning vector

Depositor

Joachim Messing

Article

Vieira et al Gene. 1982 Oct;19(3):259-68.

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

none

Available since

July 18, 2014

Availability

Academic Institutions and Nonprofits only
pPOU5F1-donor

Plasmid

#176367

Purpose

CRISPR donor plasmid to create GFP fusion proteins

Depositor

Barbara Stranger, Kevin White

Insert

POU5F1 (POU5F1 Human)

Use

CRISPR

Tags

GFP

Expression

Mutation

Promoter

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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NG0886 pCI-TPI-WT-4H

Plasmid

#65801

Purpose

NMD reporter mRNA

Depositor

Niels Gehring

Article

Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

Insert

TPI (TPI1 Human)

Use

Tags

4H (4 copies of short beta-globin 3'UTR for …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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NG0750 pCI-TPI-PTC48-4H

Plasmid

#65803

Purpose

NMD reporter mRNA

Depositor

Niels Gehring

Article

Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

Insert

TPI (TPI1 Human)

Use

Tags

4H (4 copies of short beta-globin 3'UTR for …

Expression

Mammalian

Mutation

premature stop codon at 48

Promoter

CMV

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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NG1237 pCI-TPI-PTC160-4H

Plasmid

#65804

Purpose

NMD reporter mRNA

Depositor

Niels Gehring

Article

Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

Insert

TPI (TPI1 Human)

Use

Tags

4H (4 copies of short beta-globin 3'UTR for …

Expression

Mammalian

Mutation

premature stop codon at 160

Promoter

CMV

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) ZKSCAN1 Sense

Plasmid

#60631

Purpose

Expresses the human ZKSCAN1 exon 2/3 circular RNA

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

ZKSCAN1 (ZKSCAN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-Sharpin

Plasmid

#195092

Purpose

Gene Overexpression of Human SHARPIN

Depositor

Jukka Westermarck

Article

Butt et al J Cell Sci. 2022 Oct 15;135(20):jcs26062

Insert

SHARPIN (SHARPIN Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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Arp3-TagRFP

Plasmid

#195091

Purpose

Gene Overexpression of Human Arp3

Depositor

Jukka Westermarck

Article

Butt et al J Cell Sci. 2022 Oct 15;135(20):jcs26062

Insert

Arp3 (APOBEC3A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Sharpin S178A

Plasmid

#195097

Purpose

Gene Overexpression of Human SHARPINS178A

Depositor

Jukka Westermarck

Article

Butt et al J Cell Sci. 2022 Oct 15;135(20):jcs26062

Insert

SHARPIN (SHARPIN Human)

Use

Tags

Expression

Mammalian

Mutation

S178A

Promoter

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Sharpin S131A

Plasmid

#195095

Purpose

Gene Overexpression of Human SHARPINS131A

Depositor

Jukka Westermarck

Article

Butt et al J Cell Sci. 2022 Oct 15;135(20):jcs26062

Insert

SHARPIN (SHARPIN Human)

Use

Tags

Expression

Mammalian

Mutation

S131A

Promoter

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Sharpin S146E

Plasmid

#195099

Purpose

Gene Overexpression of Human SHARPIN S146E

Depositor

Jukka Westermarck

Article

Butt et al J Cell Sci. 2022 Oct 15;135(20):jcs26062

Insert

SHARPIN (SHARPIN Human)

Use

Tags

Expression

Mammalian

Mutation

S146E

Promoter

Available since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Sharpin S285A

Plasmid

#195098

Purpose

Gene Overexpression of Human SHARPINS285A

Depositor

Jukka Westermarck

Article

Butt et al J Cell Sci. 2022 Oct 15;135(20):jcs26062

Insert

SHARPIN (SHARPIN Human)

Use

Tags

Expression

Mammalian

Mutation

S285A

Promoter

Available since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Sharpin S146A

Plasmid

#195096

Purpose

Gene Overexpression of Human SHARPINS146A

Depositor

Jukka Westermarck

Article

Butt et al J Cell Sci. 2022 Oct 15;135(20):jcs26062

Insert

SHARPIN (SHARPIN Human)

Use

Tags

Expression

Mammalian

Mutation

S146A

Promoter

Available since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-SHARPIN L276A

Plasmid

#195093

Purpose

Gene Overexpression of Human SHARPIN L276A

Depositor

Jukka Westermarck

Article

Butt et al J Cell Sci. 2022 Oct 15;135(20):jcs26062

Insert

SHARPIN (SHARPIN Human)

Use

Tags

Expression

Mammalian

Mutation

L276A

Promoter

Available since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pFS_0380_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_3_RC

Plasmid

#116992

Purpose

Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 3 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Tolypothrix campylonemoides RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0361_pET30-RT-CRISPR_Micromonospora_rosaria_ARRAY_3

Plasmid

#116973

Purpose

Expresses MrRT-Cas1-Cas2 under pT7lac promoter, encodes MrCRISPR array 3, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Micromonospora rosaria RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0379_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_3

Plasmid

#116991

Purpose

Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 3, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Tolypothrix campylonemoides RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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