We narrowed to 2,241 results for: LOB;

1. pBXMCS-2 Pconstitutive-sgRNA(Spa)_gcrA3

   Plasmid

   #133343

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets gcrA gene's promoter from Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_gcrA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   constitutive

   Available since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA

   Plasmid

   #133345

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets blaA gene's promoter from Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_blaA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   constitutive

   Available since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET15b+PCNA3 T180C

   Plasmid

   #66181

   Purpose

   Expresses the T180C mutant of Sulfolobus solfataricus PCNA3

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

   Insert

   DNA polymerase sliding clamp 3
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   T180C

   Promoter

   T7 promoter

   Available since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET15b+PCNA3 R112C

   Plasmid

   #66180

   Purpose

   Expresses the R112C mutant of Sulfolobus solfataricus PCNA3

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

   Insert

   DNA polymerase sliding clamp 3
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   R112C

   Promoter

   T7 promoter

   Available since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET15b+PCNA2 E105C

   Plasmid

   #66176

   Purpose

   Expresses the E105C mutant of Sulfolobus solfataricus PCNA2

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

   Insert

   DNA polymerase sliding clamp 2
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   E105C

   Promoter

   T7 promoter

   Available since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AF0837(1-127)

   Plasmid

   #19092

   Depositor

   SeWon Suh

   Article

   Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

   Insert

   Cell division protein pelota (DOM34 Archaeoglobus fulgidus)
   

   Use

   Tags

   C tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

7. AF0837(1-241)

   Plasmid

   #19095

   Depositor

   SeWon Suh

   Article

   Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

   Insert

   Cell division protein pelota (DOM34 Archaeoglobus fulgidus)
   

   Use

   Tags

   C tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA2

   Plasmid

   #133341

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the template strand in Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_gcrA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   constitutive

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pIVM_02

   Plasmid

   #204500

   Purpose

   Expression of VHL (54-213) for production of VCB complexes in conjunction with plasmid pIVM_26 (EloB 1-104, EloC 17-112)

   Depositor

   Alessio Ciulli

   Article

   Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.

   Insert

   amino acids 54-213 VHL (VHL Human)
   

   Use

   Tags

   6xHis, TEV, and Thrombin

   Expression

   Bacterial

   Mutation

   0

   Promoter

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFRT-PonA-BI-Gl-WT-24xMS2-PTC-18xPP7 (WP)

    Plasmid

    #189792

    Purpose

    Express wild-type and PTC-containing beta-globin genes bi-directionally under ponasteron A inducible promoter in mammalian cells

    Depositor

    Robert Singer

    Article

    Sato et al Nat Commun. 2021 Dec 10;12(1):7203. doi:

    Insert

    beta-globin (HBB Human, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ponasterone A inducible

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDUAL_tacI

    Plasmid

    #155170

    Purpose

    BaSP and CuCBP co-expression: tacI promoter controls BaSP expression

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDUAL_2_araBAD

    Plasmid

    #155176

    Purpose

    see pDUAL_araBAD: exchanged origin of replication (pBR322) and introduced bom site

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFRT-PonA-BI-Gl-GFP-PTC-mCherry-WT (5FP-WP)

    Plasmid

    #189795

    Purpose

    Express GFP/mCherry linked wild-type and PTC-containing beta-globin bi-directionally under ponasteron A inducible promoter in mammalian cells

    Depositor

    Robert Singer

    Article

    Sato et al Nat Commun. 2021 Dec 10;12(1):7203. doi:

    Insert

    beta-globin (HBB Human, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ponasterone A inducible

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFRT-PonA-BI-Gl-GFP-WT-mCherry-WT (5FP-WW)

    Plasmid

    #189794

    Purpose

    Express GFP/mCherry linked wild-type beta-globin bi-directionally under ponasteron A inducible promoter in mammalian cells

    Depositor

    Robert Singer

    Article

    Sato et al Nat Commun. 2021 Dec 10;12(1):7203. doi:

    Insert

    beta-globin (HBB Human, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ponasterone A inducible

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GammaPCDH-A3-GFP/pEGFP-N1

    Plasmid

    #197343

    Purpose

    Plasmid expressing an antigen targeting Pan-Gamma-protocadherin-Constant

    Depositor

    Joshua Weiner

    Article

    Lobas et al J Neurochem. 2012 Mar;120(6):913-27. doi

    Insert

    pcdh-gamma-A3
    

    Use

    Tags

    GFP (GFP observed)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDUAL_2_T5

    Plasmid

    #155175

    Purpose

    see pDUAL_T5: exchanged origin of replication (pBR322) and introduced bom site

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBR322-bom

    Plasmid

    #155178

    Purpose

    pBICI_2_strong without bom site

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pXGFPC-5 Pxyl-dcas9 (Spy)

    Plasmid

    #133316

    Purpose

    expresses dcas9 from Streptococcus pyogenes; repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    dcas9 (Streptococcus pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    xylose

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBICI_2_strong

    Plasmid

    #155168

    Purpose

    see pBICI_strong: exchanged origin of replication (pBR322) and introduced bom site

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX6P1‐Afu‐rnhBΔPIP

    Plasmid

    #108696

    Purpose

    For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with C-terminal PIP box deletion

    Depositor

    Martin Reijns, Andrew Jackson

    Article

    Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

    Insert

    rnhB
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    C-terminal truncation (7aa) removing PIP box

    Promoter

    tac

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge