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pJPC12-ΔM13-mCherry-P/PM,5G6T-GFP

Plasmid

#80912

Purpose

Reporter plasmid (mCherry and GFP)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

mCherry
GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

P5G6T and PM,5G6T

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 N-term PSPC1

Plasmid

#187579

Purpose

Express FLAG epitope and APEX2-tagged PSPC1 fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

PSPC1 (PSPC1 Human)

Use

Tags

APEX2-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCI-MS2V5-PABPC1 MLLEmut M161A/D165K

Plasmid

#65810

Purpose

expression of PABPC1-MS2 fusion, double mutant.

Depositor

Niels Gehring

Article

Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

Insert

PABPC1 (PABPC4 Human)

Use

Tags

MS2 stem loops and V5

Expression

Mammalian

Mutation

MLLEmut M161A/D165K

Promoter

CMV

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pEYFPC1-ECFP/GgVcl 1-419 control 1 FRET

Plasmid

#46277

Depositor

Susan Craig

Article

Chen et al J Cell Biol. 2005 May 9;169(3):459-70.

Insert

ECFP + Vcl 1-419 (VCL Chicken)

Use

Tags

EYFP

Expression

Mammalian

Mutation

aa 1-419

Promoter

CMV

Available since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only

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Anti-TrpC1 [1F1R-rat IgG2a] - Chimeric

Plasmid

#231834

Purpose

Mammalian expression plasmid of anti-TrpC1 (Human) rat IgG2a R-mab. Derived from hybridoma 1F1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-TrpC1 (Homo sapiens) recombinant mouse monoclonal antibody (TRPC1 Chimera: M. musculus (mouse)/R. norvegicus (rat))

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pC131: pAAV.CMV-CasRx-VEGFA sgRNA array

Plasmid

#203444

Purpose

Plasmid expressing active RfxCas13d with an array of three VEGFA mRNA targeting gRNAs

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA array
RfxCas13d

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1-RRM1-RRM2-V5His6_H

Plasmid

#146490

Purpose

Insect Expression of DmPABPC1-RRM1-RRM2

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Sep 21. ():.

Insert

DmPABPC1-RRM1-RRM2 (pAbp Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1-RRM2-RRM3-V5His6_H

Plasmid

#146491

Purpose

Insect Expression of DmPABPC1-RRM2-RRM3

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Sep 21. ():.

Insert

DmPABPC1-RRM2-RRM3 (pAbp Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1-RRM3-RRM4-V5His6_H

Plasmid

#146492

Purpose

Insect Expression of DmPABPC1-RRM3-RRM4

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Sep 21. ():.

Insert

DmPABPC1-RRM3-RRM4 (pAbp Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1_90-635-siRNAres-V5His6_L

Plasmid

#146860

Purpose

Insect Expression of DmPABPC1_90-635-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmPABPC1_90-635-siRNAres (pAbp Fly)

Use

Tags

Expression

Insect

Mutation

five silent mutations compared to the sequence gi…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1-del91-164-V5His6_L

Plasmid

#146861

Purpose

Insect Expression of DmPABPC1_del91-164

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmPABPC1_del91-164 (pAbp Fly)

Use

Tags

Expression

Insect

Mutation

one non silent mutation G26S, and five silent mut…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1-del183-258-siRNAres-V5His6_L

Plasmid

#146862

Purpose

Insect Expression of DmPABPC1_del183-258-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmPABPC1_del183-258-siRNAres (pAbp Fly)

Use

Tags

Expression

Insect

Mutation

one non silent mutation G26S, and seven silent mu…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1-del288-359-siRNAres-V5His6_L

Plasmid

#146863

Purpose

Insect Expression of DmPABPC1_del288-359-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmPABPC1_del288-359-siRNAres (pAbp Fly)

Use

Tags

Expression

Insect

Mutation

one non silent mutation G26S, and six silent muta…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1-del364-550-siRNAres-V5His6_L

Plasmid

#146864

Purpose

Insect Expression of DmPABPC1_del364-550-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmPABPC1_del364-550-siRNAres (pAbp Fly)

Use

Tags

Expression

Insect

Mutation

one non silent mutation G26S, and five silent mut…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFPC1/GgVcl 1-1066 A50I K1306A, R1308A mutant

Plasmid

#46267

Depositor

Susan Craig

Insert

Vcl (VCL Chicken)

Use

Tags

EGFP

Expression

Mammalian

Mutation

K1306A, R1308A, A50I mutation; talin binding defi…

Promoter

CMV

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pBBK67 pHomL-RPL18Bp-RPC19-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179051

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of RPC19-Link-Scarlet(rfp), a marker for the nucleolus

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-RPC19-Link-Scarlet(rfp)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK46 pHomL-RPL18Bp-RPC19-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179030

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of RPC19-Link-Neon(gfp), a marker for the nucleolus

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-RPC19-Link-Neon(gfp)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO CD16.7-h-PKD1 FLM (4078-4169)

Plasmid

#83463

Purpose

Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and PKD1 sequence that corresponds to 4078 to 4169 of PC1 (96)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Use

Tags

CD16.7

Expression

Mammalian

Mutation

Amino-acids 4078-4169

Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO CD16.7-h-PKD1 FLM (4078-4303)

Plasmid

#83465

Purpose

Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and PKD1 sequence that corresponds to amino acids 4078 to 4303 of PC1 (98)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Use

Tags

CD16.7

Expression

Mammalian

Mutation

Amino-acids 4078-4303

Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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AF20-double-tag

Plasmid

#108389

Purpose

Express human PC1

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

PKD1 (PKD1 Human)

Use

Tags

eGFP-TEV cleavage-S15 and mcherry-TEV cleavage-V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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