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pBoL1P3GA

Plasmid

#112909

Purpose

Express a cloned sgRNA from AtU626 promoter

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2019;1917:155-170. doi

Insert

AtU626-LACZ-sgRNA

Use

Tags

Expression

Mutation

Promoter

At U626

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLJM1-CRY2-mCherry-(GGGGS)3-hRGS2box

Plasmid

#112257

Purpose

Expresses RGS component of opto-RGS2 in mammalian cells

Depositor

Brian Chow

Article

Hannanta-Anan et al ACS Synth Biol. 2018 Jun 15;7(6):1488-14

Insert

CRY2-mCherry-(GGGGS)3-hRGS2box

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-PdCO-mScarlet

Plasmid

#198507

Purpose

Expresses optimized PdCO in frame with mScarlet under control of CMV promotor.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Insert

PdCO

Use

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-PdCO-EGFP

Plasmid

#198512

Purpose

Expresses optimized PdCO in frame with EGFP under control of CMV promotor.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Insert

PdCO

Use

Tags

EGFP and Rho1D4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAGT8162 (AATG_dttLbCas12a-i_TTCG)

Plasmid

#192350

Purpose

L0 MoClo-compatible GoldenGate-module of dttLbCas12a-i for in planta expression

Depositor

Tom Schreiber

Article

Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

Insert

AATG_dttLbCas12a-i_TTCG

Use

CRISPR

Tags

Expression

Mutation

D156R; E925Q

Promoter

Available since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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Py2087ACR1

Plasmid

#153032

Purpose

Anion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).

Depositor

Peter Hegemann

Article

Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.

Insert

Py2087ACR1

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV (+ enhancer)

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pGG-PIP64

Plasmid

#196802

Purpose

Contains RPS4 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

RESISTANT TO P. SYRINGAE 4 (RPS4 Mustard Weed)

Use

Greengate (gg) entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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PymeACR1

Plasmid

#153031

Purpose

Prasinophyte anion-conducting channelrhodopsin. Codon-optimized for mammalian expression (human/mouse).

Depositor

Peter Hegemann

Article

Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.

Insert

PymeACR1

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV (+ enhancer)

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pTol1-UAS:ChrimsonR-tdTomato

Plasmid

#124231

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChrimsonR fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Article

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

ChrimsonR-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Expression

Mutation

Promoter

14xUAS-E1b

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pTol1-UAS:GtACR2-tdTomato

Plasmid

#124236

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin GtACR2 fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Article

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

GtACR2-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Expression

Mutation

Promoter

14xUAS-E1b

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pTol1-UAS:CoChR-tdTomato

Plasmid

#124233

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CoChR fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Article

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

CoChR-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Expression

Mutation

Promoter

14xUAS-E1b

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAGM51323

Plasmid

#153224

Purpose

Level 1 position 2 containing the Arabidopsis Rps5a promoter, Cas9 (Zcasi), and the nos terminator

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

Arabidopsis Rps5a promoter, Cas9 (Zcasi), nos terminator

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pTol1-UAS:GtACR1-tdTomato

Plasmid

#124235

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin GtACR1 fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Article

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

GtACR1-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Expression

Mutation

Promoter

14xUAS-E1b

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pTol1-UAS:Chronos-tdTomato

Plasmid

#124232

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin Chronos fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Article

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

Chronos-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Expression

Mutation

Promoter

14xUAS-E1b

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChrimsonR-LSSmOrange

Plasmid

#149691

Purpose

For packaging and expression of ChrimsonR-LSSmOrange in neurons via AAV

Depositor

Robert Malenka

Article

Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

Insert

ChrimsonR

Use

AAV

Tags

LSSmOrange

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLBC0120

Plasmid

#172791

Purpose

Level 0 module containing CDS2ns_mHSP21

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS mature HSP21 (44-227), Arabidopsis thaliana

Use

Synthetic Biology

Tags

Expression

Mutation

no

Promoter

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0130

Plasmid

#172792

Purpose

Level 0 module containing CDS2ns_mPTAC5

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS mature PTAC5 (41-387), Arabidopsis thaliana

Use

Synthetic Biology

Tags

Expression

Mutation

no

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV EF1a DIO iChloC 2A dsRed

Plasmid

#70762

Purpose

An improved chloride-conducting channelrhodopsin for light-induced inhibition of neuronal activity in vivo. Cre inducible expression (double floxed inversed ORF)

Depositor

Thomas Oertner

Article

Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103

Inserts

Channelrhodopsin-2
red fluorescent protein

Use

AAV

Tags

Expression

Mammalian

Mutation

E83Q,E90R,E101S,D156N,T159C

Promoter

EF1a

Available since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE1109

Plasmid

#202773

Purpose

CRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thaliana

Depositor

Johannes Stuttmann

Article

Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

Inserts

FAST marker
pRPS5a:TREX2-zCas9_ttriple
ccdB cassette (BsaI-excisable)

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CIBN-CreC

Plasmid

#75267

Purpose

The first half of the photo-activatable cre recombinase

Depositor

Michael Bruchas

Article

Schindler et al Sci Rep. 2015 Sep 9;5:13627. doi: 10.103

Insert

N-terminal portion of the CIB1 transcription factor

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only

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