We narrowed to 7,491 results for: RAP

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pBiT3.1-C_STRAP:1-350

Plasmid

#211180

Purpose

Mammalian expression of full-length human STRAP. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

STRAP:M1-A350 (STRAP Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_WRAP73:1-460

Plasmid

#211307

Purpose

Mammalian expression of full-length human WRAP73. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WRAP73:M1-T460 (WRAP73 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_WRAP53:1-548

Plasmid

#211306

Purpose

Mammalian expression of full-length human WRAP53. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WRAP53:M1-I548 (WRAP53 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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phAG-MCL-CDK5RAP3

Plasmid

#204571

Purpose

Expresses hAG-tagged CDK5RAP3 in mammalian cells

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

CDK5RAP3 (AMY2A Human)

Expression

Mammalian

Mutation

none

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAsh-MCL-GABARAP

Plasmid

#204574

Purpose

Expresses Ash-tagged GABARAP in mammalian cells

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

GABARAP (GABARAP Human)

Expression

Mammalian

Mutation

deltaL117

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-RAPH1Δ536-587

Plasmid

#194865

Purpose

Express eGFP-RAPH1 lacking amino acids 536-587 in mammalian cells. This constructs does not localise to filopodia anymore.

Depositor

Guillaume Jacquemet

Article

Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

Insert

RAPH1 (RAPH1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

RAPH1 with amino acids 536-587 deleted

Promoter

CMV

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pLPC-FLAG.HA.HA.Mm.RAP1.I312R

Plasmid

#184643

Purpose

Retroviral expressions of mouse RAP1 I312R

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

rap1

Use

Retroviral

Tags

HA FLAG

Expression

Mammalian

Mutation

I312R

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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Rap-MESA-20-Tev

Plasmid

#84555

Purpose

MESA protease chain with rapamycin FKBP ectodomain, 20 extracellular linkers, and Tev protease

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

Rap-MESA-FKBP-20-Tev

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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RAP2A-p2A-E2Crimson

Plasmid

#83940

Purpose

Lentiviral vector expressing RAP2A-p2A-E2Crimson

Depositor

David Sabatini

Article

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Tags

p2A-E2Crimson

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223 GABARAPL1 no-stop

Plasmid

#123210

Purpose

Gateway entry clone encoding human GABARAPL1 lacking stop codon, suitable for C-terminal tagging

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Use

Gateway entry vector / entry clone

Mutation

Stop codon removed

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 GABARAPL2 no-stop

Plasmid

#123211

Purpose

Gateway entry clone encoding human GABARAPL2 lacking stop codon, suitable for C-terminal tagging

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)

Use

Gateway entry vector / entry clone

Mutation

Stop codon removed

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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CDK5RAP2 F11.3 gRNA

Plasmid

#90606

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK5RAP2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CDK5RAP2 (Guide Designation F11.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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CDK5RAP2 F12.3 gRNA

Plasmid

#90607

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK5RAP2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CDK5RAP2 (Guide Designation F12.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pGAPTrap-Clover-Muro

Plasmid

#82334

Purpose

Vector targeting the Clover gene to the GAPDH locus of human cells. Clover is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.

Depositor

Ed Stanley

Article

Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

Insert

Clover-T2AMuro

Use

Synthetic Biology

Expression

Mammalian

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pGAPTrap-eGFP-IRESMeo

Plasmid

#82502

Purpose

Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. G418 resistance is encoded by the Meo gene.

Depositor

Ed Stanley

Article

Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

Insert

eGFP-IRESMeo

Use

Synthetic Biology

Expression

Mammalian

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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TRRAP gRNA (BRDN0001146781)

Plasmid

#76536

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRRAP

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRRAP (TRRAP Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TRRAP gRNA (BRDN0001148174)

Plasmid

#76538

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRRAP

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRRAP (TRRAP Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcFLAG-Grap2-R83E

Plasmid

#74512

Purpose

Overexpression of Grap2 R83E in mammalian cells

Depositor

Lars Rönnstrand

Article

Chougule et al Oncotarget. 2016 Feb 15. doi: 10.18632/o

Insert

Grap2 (GRAP2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

R83E

Promoter

CMV

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
miR-451 Trap

Plasmid

#61487

Purpose

to knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of bulged trap sequences cloned 3' to GFP under the control of a biPGK promoter

Depositor

Curt Civin

Article

Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

Insert

miR-451 Trap

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

bidirectional phosphoglycerate kinase promoter (b…

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only
hSRAP.HMG6.wt.Dlnk.a13-236

Plasmid

#49814

Purpose

expression vector for MBP-SRAP(13-236) fusion

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator protein (SRA1 Human)

Tags

E. coli Maltose Binding Protein

Expression

Bacterial

Mutation

wildtype codon-optimized for E. coli expression

Promoter

T7

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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