We narrowed to 7,125 results for: RAP

1. pBiT3.1-C_WRAP73:1-460

   Plasmid

   #211307

   Purpose

   Mammalian expression of full-length human WRAP73. C-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   WRAP73:M1-T460 (WRAP73 Human)
   

   Use

   Tags

   GSSGGSSGVSGWRLFKKIS

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBiT3.1-C_WRAP53:1-548

   Plasmid

   #211306

   Purpose

   Mammalian expression of full-length human WRAP53. C-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   WRAP53:M1-I548 (WRAP53 Human)
   

   Use

   Tags

   GSSGGSSGVSGWRLFKKIS

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. phAG-MCL-CDK5RAP3

   Plasmid

   #204571

   Purpose

   Expresses hAG-tagged CDK5RAP3 in mammalian cells

   Depositor

   Masaaki Komatsu

   Article

   Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

   Insert

   CDK5RAP3 (AMY2A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAsh-MCL-GABARAP

   Plasmid

   #204574

   Purpose

   Expresses Ash-tagged GABARAP in mammalian cells

   Depositor

   Masaaki Komatsu

   Article

   Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

   Insert

   GABARAP (GABARAP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deltaL117

   Promoter

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-RAPH1Δ536-587

   Plasmid

   #194865

   Purpose

   Express eGFP-RAPH1 lacking amino acids 536-587 in mammalian cells. This constructs does not localise to filopodia anymore.

   Depositor

   Guillaume Jacquemet

   Article

   Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

   Insert

   RAPH1 (RAPH1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   RAPH1 with amino acids 536-587 deleted

   Promoter

   CMV

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLPC-FLAG.HA.HA.Mm.RAP1.I312R

   Plasmid

   #184643

   Purpose

   Retroviral expressions of mouse RAP1 I312R

   Depositor

   Agnel Sfeir

   Article

   Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

   Insert

   rap1
   

   Use

   Retroviral

   Tags

   HA FLAG

   Expression

   Mammalian

   Mutation

   I312R

   Promoter

   CMV

   Available since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Rap-MESA-20-Tev

   Plasmid

   #84555

   Purpose

   MESA protease chain with rapamycin FKBP ectodomain, 20 extracellular linkers, and Tev protease

   Depositor

   Joshua Leonard

   Article

   Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

   Insert

   Rap-MESA-FKBP-20-Tev
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. RAP2A-p2A-E2Crimson

   Plasmid

   #83940

   Purpose

   Lentiviral vector expressing RAP2A-p2A-E2Crimson

   Depositor

   David Sabatini

   Article

   Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

   Insert

   RAP2A (RAP2A Human)
   

   Use

   Lentiviral

   Tags

   p2A-E2Crimson

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223 GABARAPL1 no-stop

   Plasmid

   #123210

   Purpose

   Gateway entry clone encoding human GABARAPL1 lacking stop codon, suitable for C-terminal tagging

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
   

   Use

   Gateway entry vector / entry clone

   Tags

   Expression

   Mutation

   Stop codon removed

   Promoter

   Available since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR223 GABARAPL2 no-stop

    Plasmid

    #123211

    Purpose

    Gateway entry clone encoding human GABARAPL2 lacking stop codon, suitable for C-terminal tagging

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
    

    Use

    Gateway entry vector / entry clone

    Tags

    Expression

    Mutation

    Stop codon removed

    Promoter

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. CDK5RAP2 F11.3 gRNA

    Plasmid

    #90606

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK5RAP2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    CDK5RAP2 (Guide Designation F11.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. CDK5RAP2 F12.3 gRNA

    Plasmid

    #90607

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK5RAP2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    CDK5RAP2 (Guide Designation F12.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGAPTrap-Clover-Muro

    Plasmid

    #82334

    Purpose

    Vector targeting the Clover gene to the GAPDH locus of human cells. Clover is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    Clover-T2AMuro
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGAPTrap-eGFP-IRESMeo

    Plasmid

    #82502

    Purpose

    Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. G418 resistance is encoded by the Meo gene.

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    eGFP-IRESMeo
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. TRRAP gRNA (BRDN0001146781)

    Plasmid

    #76536

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRRAP

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRRAP (TRRAP Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TRRAP gRNA (BRDN0001148174)

    Plasmid

    #76538

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRRAP

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRRAP (TRRAP Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcFLAG-Grap2-R83E

    Plasmid

    #74512

    Purpose

    Overexpression of Grap2 R83E in mammalian cells

    Depositor

    Lars Rönnstrand

    Article

    Chougule et al Oncotarget. 2016 Feb 15. doi: 10.18632/o

    Insert

    Grap2 (GRAP2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    R83E

    Promoter

    CMV

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

18. miR-451 Trap

    Plasmid

    #61487

    Purpose

    to knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of bulged trap sequences cloned 3' to GFP under the control of a biPGK promoter

    Depositor

    Curt Civin

    Article

    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

    Insert

    miR-451 Trap
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    bidirectional phosphoglycerate kinase promoter (b…

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

19. hSRAP.HMG6.wt.Dlnk.a13-236

    Plasmid

    #49814

    Purpose

    expression vector for MBP-SRAP(13-236) fusion

    Depositor

    Thomas Cech

    Article

    McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

    Insert

    steroid receptor RNA activator protein (SRA1 Human)
    

    Use

    Tags

    E. coli Maltose Binding Protein

    Expression

    Bacterial

    Mutation

    wildtype codon-optimized for E. coli expression

    Promoter

    T7

    Available since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. hSRAP.TWIN1.a111-215

    Plasmid

    #49820

    Purpose

    expression vector for SRAP(111-215) in pTWIN1 vector

    Depositor

    Thomas Cech

    Article

    McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

    Insert

    steroid receptor RNA activator protein aa 111-215 (SRA1 Human)
    

    Use

    Tags

    intein & chitin binding domain

    Expression

    Bacterial

    Mutation

    wildtype codon-optimized for E. coli expression

    Promoter

    T7

    Available since

    Jan. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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