We narrowed to 12,173 results for: SHA;

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pBABE myc-Raptor S722A/S792A

Plasmid

#18117

Depositor

Reuben Shaw

Article

Gwinn et al Mol Cell. 2008 Apr 25. 30(2):214-26.

Insert

Raptor (RPTOR Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

S722A, S792A

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pGEX-5X2-RalBP1

Plasmid

#37245

Depositor

Christopher Counter, David Kashatus

Article

Kashatus et al Nat Cell Biol. 2011 Aug 7;13(9):1108-15.

Insert

RalBP1 (RALBP1 Human)

Tags

GST

Expression

Bacterial

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
pRS159

Plasmid

#122378

Purpose

CRISPRi plasmid for use in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)

Depositor

Rebecca Shapiro

Article

Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pAS1-Prig-3::tagBFP

Plasmid

#165598

Purpose

rig-3 promoter derive BFP expression in AVA head neurons.

Depositor

Andrew Leifer

Article

Hallinen et al Elife. 2021 Jul 29;10:e66135. doi: 10.75

Insert

Prig-3, tagBFP

Expression

Worm

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRS143

Plasmid

#122377

Purpose

CRISPRi plasmid for use in Candida albicans. Contains dCas9 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)

Depositor

Rebecca Shapiro

Article

Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pSLC-284

Plasmid

#124294

Purpose

A low copy plasmid constitutively expressing vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.

Depositor

Swaine Chen

Article

Eshaghi et al Pathogens. 2016 Jan 5;5(1). pii: pathoge

Insert

vsfGFP-9

Expression

Bacterial

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pRS267

Plasmid

#122380

Purpose

CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -112 bp upstream of HSP90 start codon

Depositor

Rebecca Shapiro

Article

Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

Insert

HSP90

Use

CRISPR

Expression

Yeast

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pQP-CiRISB

Plasmid

#138978

Purpose

Light-controlled tri-directional targeting of endogenous proteins (cytoplasm-shifted)

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-BphP1-CAAX-IRES2-iB(GFP)-NESmCherry- Q-PAS1-AsLOV2cNLS-NotI

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pRS263

Plasmid

#122379

Purpose

CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -141 bp upstream of HSP90 start codon

Depositor

Rebecca Shapiro

Article

Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

Insert

HSP90

Use

CRISPR

Expression

Yeast

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pQP-2473

Plasmid

#138976

Purpose

Nucleus-to-cytoplasm relocalization of genomically expressed GFP-fusion using NIR light controlled intrabody.

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-BphP1-NES-T2A- iB(GFP)-NES-mCherry-QPAS1- NLS-NotI

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pQP-AR10

Plasmid

#138980

Purpose

Endogenous RAS regulation using light-controlled intrabody

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

BglII-BphP1-NES-T2A- iB(RAS)-NES-mCherry-QPAS1- NLS-NotI

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pQP-NiRISB

Plasmid

#138977

Purpose

Light-controlled tri-directional targeting of endogenous proteins (nucleus-shifted)

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-BphP1-CAAX-IRES2-NES- iB(GFP)- mCherry-Q-PAS1-AsLOV2cNLS-NotI

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pQP-iRISB

Plasmid

#138979

Purpose

Light-controlled tri-directional targeting of endogenous proteins

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-BphP1-CAAX-IRES2-NES-SGlinkeriB( GFP)-mCherry-Q-PAS1-AsLOV2cNLS-NotI

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pQP-2363

Plasmid

#138975

Purpose

Relocalization of genomically expressed protein of interest from cytoplasm to nucleus upon near-infrared illumination

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-BphP1- iB(GFP) -IRES2-NES-mCherry-QPAS1- NLS-NotI

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-V5-DGCR8

Plasmid

#51383

Depositor

Narry Kim

Article

Yeom et al Nucleic Acids Res. 2006;34(16):4622-9. E

Insert

DGCR8 (DGCR8 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pRS156

Plasmid

#182707

Purpose

CRISPRa plasmid for Candida albicans (dCas9-VPR and sgRNA cloning site)

Depositor

Rebecca Shapiro

Article

Gervais et al G3 (Bethesda). 2022 Dec 1:jkac301. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pQP-2460

Plasmid

#138973

Purpose

Light-induced recruitment of QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NES-mCherry-Q-PAS1-NotI

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pRSETC-PITPbeta (splice variant 1)

Plasmid

#58270

Purpose

bacterial expression of PITP beta (phosphatidylinositol transfer protein beta)

Depositor

Shamshad Cockcroft

Article

Shadan et al Traffic. 2008 Sep;9(10):1743-56. doi: 10

Insert

PITP beta (PITPNB Human)

Tags

HIS

Expression

Bacterial

Promoter

T7

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
NmMetNI-WT

Plasmid

#172114

Purpose

Expression of MetN and MetI membrane proteins

Depositor

Douglas Rees

Article

Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:

Inserts

MetN
MetI

Tags

10x His Tag and Enterokinase

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pQP-2376

Plasmid

#138974

Purpose

Light-induced recruitment of NSImb-QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NSImb-NES-mCh-Q-PAS1-NotI

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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