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Anti-Kir2.4 K+ channel [N465/11]

Plasmid

#190332

Purpose

Mammalian Expression Plasmid of anti-Kir2.4 K+ channel (Mouse). Derived from hybridoma N465/11.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Kir2.4 K+ channel (Mus musculus) recombinant Mouse monoclonal antibody (Kcnj14 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hCHAT)-PGKpuro2ABFP-W

Plasmid

#163147

Purpose

Lentiviral gRNA plasmid targeting human CHAT gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

CHAT (CHAT Human)

Use

CRISPR and Lentiviral

Tags

BFP

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-KChIP1 K+ channel [K55/7R]

Plasmid

#188225

Purpose

Mammalian Expression Plasmid of anti-KChIP1 K+ channel (Rat). Derived from hybridoma K55/7

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-KChIP1 K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Kcnip1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Kv2.1 K+ channel [D4/40R]

Plasmid

#182096

Purpose

Mammalian Expression Plasmid of anti-Kv2.1 K+ channel (Rat). Derived from hybridoma D4/40.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Kv2.1 K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Kcnb1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Kv2.2 K+ channel [N372B/1R]

Plasmid

#182112

Purpose

Mammalian Expression Plasmid of anti-Kv2.2 K+ channel (Rat). Derived from hybridoma N372B/1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Kv2.2 K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Kcnb2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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Kv1.2 K+ channel scFv [K14/16]

Plasmid

#182064

Purpose

Mammalian Expression of Kv1.2 K+ channel scFv. Derived from hybridoma K14/16.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Kv1.2 K+ channel (Homo sapiens) (KCNA2 Mouse)

Use

Tags

HA, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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Kv2.1 K+ channel scFv [K89/34]

Plasmid

#182069

Purpose

Mammalian Expression of Kv2.1 K+ channel scFv. Derived from hybridoma K89/34.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Kv2.1 K+ channel (Rattus norvegicus) (Kcnb1 Mouse)

Use

Tags

HA, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCxcl1-Firefly-Chantico-Tdel

Plasmid

#163900

Purpose

Luciferase reporter for mouse Cxcl1 with Chantico promoter Tbx5 site deletion

Depositor

Alexander Ruthenburg

Article

Hall et al bioRxiv 2021.12.23.474040

Insert

Luciferase

Use

Luciferase

Tags

Expression

Mutation

Promoter

Cxcl1

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB2lock-no charge

Plasmid

#160532

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-no charge

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, E337A, N338A, K341A, N342A

Promoter

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB2lock-charge+

Plasmid

#160531

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-charge+

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, Y336K, L340K

Promoter

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB1lock-no charge

Plasmid

#160525

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB1lock-no charge

Use

Tags

His-Tag

Expression

Bacterial

Mutation

A221C, N404C, E337A, N338A, K341A, N342A

Promoter

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB1lock-charge+

Plasmid

#160524

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB1lock-charge+

Use

Tags

His-Tag

Expression

Bacterial

Mutation

A221C, N404C, Y336K, L340K

Promoter

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-prepore-charge+

Plasmid

#160522

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-prepore-charge+

Use

Tags

His-Tag

Expression

Bacterial

Mutation

I104C, G244C, Y336K, L340K

Promoter

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Kv1.5 K+ channel [K7/45R]

Plasmid

#140069

Purpose

Mammalian Expression Plasmid of anti-Kv1.5 K+ channel (Rat). Derived from hybridoma K7/45.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Kv1.5 K+ channel (Rat) recombinant mouse monoclonal antibody (Kcna5 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-R2L3-cHA-IRESpuro-pA

Plasmid

#139537

Purpose

Provides HA protein tag N-terminal to IRES-puro-pA cassette on Gateway entry vector

Depositor

Minoru Ko

Insert

HA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pMH-HT_CtCHAD-R438A-K441A

Plasmid

#127791

Purpose

Expresses a CtCHAD mutant protein in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

CtCHAD (CT0884 Chlorobium tepidum)

Use

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Residues 208-522 (CHAD domain), mutations in Arg4…

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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KA1063_pPB-hCMV*1-cHA-IRESH2BmKO2

Plasmid

#124169

Purpose

piggyBac-based Dox-inducible expression

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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KA1123_pPB-TREtight-cHA-IRESH2BmKO2

Plasmid

#124172

Purpose

piggyBac-based Dox-inducible expression

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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KA0718_pPB-TREtight-cHA-IRESVenus

Plasmid

#124171

Purpose

piggyBac-based Dox-inducible expression

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-SIRPT1-Latimeria chalumnae

Plasmid

#75324

Purpose

GST fusion Latimeria chalumnae Sacsin 94-1368

Depositor

Kalle Gehring

Insert

Sacsin Latimeria chalumnae 94-1368

Use

Tags

GST-Tag

Expression

Bacterial

Mutation

Promoter

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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