Addgene

Showing: 241 - 260 of 7084 results

Results per page:

Sort results by:

pEF.myc.ER-E2-Crimson

Plasmid

#38770

Purpose

In vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)

Depositor

Benjamin Glick

Article

Strack et al Biochemistry. 2009 Sep 8;48(35):8279-81.

Insert

E2-Crimson

Use

Tags

ER retention signal (KDEL), ER signal sequence (M…

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
ecAT3.10

Plasmid

#107215

Purpose

Mammalian expression plasmid to target the ecAT3.10 extracellular ATP sensor to the cell surface.

Depositor

Mathew Tantama

Article

Conley et al PLoS One. 2017 Nov 9;12(11):e0187481. do

Insert

ecAT3.10

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-iGlu3Fast

Plasmid

#226500

Purpose

Ultrafast-decay iGluSnFR3 variant for tracking high frequency synaptic glutamate release

Depositor

Katalin Torok

Article

Tran et al bioRxiv, August 30th, 2024

Insert

iGluSnFR3 S72T

Use

Tags

IgK, Myc tag, and PDGFR Beta TM domain

Expression

Mammalian

Mutation

S72T

Promoter

CMV

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-TREtight-mTagBFP2-N2cG

Plasmid

#192838

Purpose

plasmid for making helper virus for monosynaptic tracing using CVS-N2c rabies virus; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA

Depositor

Ian Wickersham

Insert

mTagBFP2 and rabies virus CVS-N2c strain glycoprotein genes (N2cG)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TREtight

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTn7-SCOUT13.G

Plasmid

#201001

Purpose

pLv1-pUC18R6KT-miniTn7-Tc-sfGFP, TcR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Insert

superfolder GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23104

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
LT0

Plasmid

#210551

Purpose

Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT0 reporter

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

pU6::AAVS1gRNA::LT0::mCherrySSM

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
LT1

Plasmid

#210552

Purpose

Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT1 reporter

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

pU6::AAVS1gRNA::LT1::mCherrySSM

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
LT2

Plasmid

#210553

Purpose

Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT2 reporter

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

pU6::AAVS1gRNA::LT2::mCherrySSM

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
YIPlac204TKC-E2-Crimson-HDEL

Plasmid

#38771

Depositor

Benjamin Glick

Article

Strack et al Biochemistry. 2009 Sep 8;48(35):8279-81.

Insert

E2-Crimson

Use

Tags

Linker peptide and yeast ER retention signal sequ…

Expression

Yeast

Mutation

Promoter

TPI1

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
LT1_34_sfpHluorin

Plasmid

#177707

Purpose

Plasmid for genome integration in X2 site expressing sfpHluorin (intracellular pH biosensor)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

pTEF1-sfpHluorin

Use

Used as donor in genome integration after lineari…

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
DriD-wHTH-DNABD

Plasmid

#237851

Purpose

The wHTH DNA binding domain used to understand the allosteric mechanism

Depositor

Maria Schumacher

Article

Cannistraci et al Commun Biol. 2025 Apr 29;8(1):679. doi:

Insert

DriD wHTH DNABD

Use

Tags

Expression

Mutation

Promoter

Available since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
DriD-3HB-mutant

Plasmid

#237852

Purpose

DriD 3HB surface mutant used to interrogate the allosteric mechanism

Depositor

Maria Schumacher

Article

Cannistraci et al Commun Biol. 2025 Apr 29;8(1):679. doi:

Insert

DriD 3HB mutant

Use

Tags

Expression

Mutation

Promoter

Available since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p323-L10A-PATagRFP

Plasmid

#74172

Purpose

Fluorescent reporter for single ribosome tracking

Depositor

Robert Singer

Article

Katz et al Elife. 2016 Jan 13;5. pii: e10415. doi:

Insert

ribosomal protein L10A (Rpl10a Rat)

Use

Lentiviral

Tags

PATagRFP

Expression

Mutation

Promoter

UbC

Available since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
V2-MESA-35F-M-dCas9

Plasmid

#84506

Purpose

MESA target chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, M cleavage sequence, and dCas9-VP64

Depositor

Joshua Leonard

Article

Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

Insert

V2-MESA-35F-M-dCas9

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
MCP/PCP RNA Lantern

Plasmid

#226095

Purpose

RNA tracking

Depositor

Jennifer Prescher

Article

Halbers et al Nat Commun. 2024 Nov 18;15(1):9992. doi:

Insert

NLS-HA-MS2-SmBiT-IRES-PP7-LgBiT-FLAG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CJ8421_04975 Rptr2 and CJ8421_04975 mRNA

Plasmid

#194210

Purpose

Expresses the Campylobacter mRNA CJ8421_04975 and the mRNA-targeting reprogrammed tracrRNA 2

Depositor

Chase Beisel

Article

Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

Insert

CJ8421_04975 mRNA and CJ8421_04975 Rptr2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
RNA-Protein Landing Pad

Plasmid

#226162

Purpose

Used to generate a landing pad in Flp-In cells containing promoters for protein and MS2-circRNA barcode expression

Depositor

Dustin Maly

Article

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Inserts

mTagBFP2-P2A-iCasp9
Reverse tetracycline transactivator 3
Hygromycin-IRES-EGFP

Use

Synthetic Biology

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFlp-Tc

Plasmid

#222336

Purpose

flp-cl-sacB-pL1V-Lv1-TetAR-pBBR1-ELT3

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Inserts

sacB (sacB )
flp (flp )
cI (cI )

Use

Tags

Expression

Mutation

Promoter

cl, flp, and sacB

Available since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-eLACCO1.1

Plasmid

#167944

Purpose

Fluorescent biosensor for extracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

Insert

eLACCO1.1

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pADV-Sec61b-TurboID

Plasmid

#179143

Purpose

Recombinant adenoviral plasmid for Sec61b-TurboID expression

Depositor

Jae Myoung Suh

Article

Kim et al Nat Commun. 2021 Sep 1;12(1):5204. doi:

Insert

Sec61b-V5-TurboID

Use

Adenoviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge