We narrowed to 17,082 results for: EMB

1. pBTK110

   Plasmid

   #110576

   Purpose

   BTK Type 2 promoter for golden gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   CP6 Promoter with RBS
   

   Use

   Synthetic Biology

   Available Since

   Feb. 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pBTK112

   Plasmid

   #110577

   Purpose

   BTK Type 2 promoter for golden gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   CP12b Promoter with RBS
   

   Use

   Synthetic Biology

   Available Since

   Feb. 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pBTK113

   Plasmid

   #110578

   Purpose

   BTK Type 2 promoter for golden gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   CP32 Promoter with RBS
   

   Use

   Synthetic Biology

   Available Since

   Feb. 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. KHLH31 pEGFP-N2 (1089)

   Plasmid

   #62032

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

   Insert

   KHLH31
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

5. 719-2µ-minHIS3

   Plasmid

   #40610

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Insert

   HIS3 (HIS3 Budding Yeast, Synthetic)
   

   Tags

   HA

   Expression

   Yeast

   Mutation

   Every codon of the coding sequence has been repla…

   Promoter

   TDH3

   Available Since

   Dec. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAV10

   Plasmid

   #63213

   Purpose

   pUC19 backbone (Amp), contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. NotI or FseI sites for TU digest.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pF(UG) hSyn NS34a-msGFP-'S-mito

   Plasmid

   #158768

   Purpose

   Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

   Depositor

   Edwin Chapman

   Article

   Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

   Insert

   NS3/4a protease and msGFP
   

   Use

   Lentiviral

   Mutation

   Flexible linkers between the protease and msGFP a…

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti6-CENP-A-mCherry

   Plasmid

   #89767

   Purpose

   Lentivirus destination vector for stable expression of mCherry-tagged kinetochore marker CENP-A

   Depositor

   Torsten Wittmann

   Article

   Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

   Insert

   CENP-A (CENPA Human)
   

   Use

   Lentiviral

   Tags

   3xHA and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTH727-CEN-RLuc/staCFLuc

   Plasmid

   #38211

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Inserts

   Firefly Luciferase
   Renilla luciferase
   

   Expression

   Yeast

   Mutation

   The last three codons of the full-length Firefly …

   Promoter

   ADH1 and TDH3 (=GPD)

   Available Since

   Oct. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEGFP VAMP8

    Plasmid

    #42311

    Depositor

    Thierry Galli

    Article

    Paumet et al J Immunol. 2000 Jun 1;164(11):5850-7.

    Insert

    VAMP 8 (VAMP8 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. NET5s pEGFP-N2 (1154)

    Plasmid

    #62034

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

    Insert

    NET5s (TMEM201 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    short form

    Promoter

    CMV

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

12. NET5L pEGFP-N2 (1155)

    Plasmid

    #62035

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    NET5L (TMEM201 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    long form

    Promoter

    CMV

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

13. TPC2-G-GECO1.2

    Plasmid

    #207141

    Purpose

    Fusion of Ca2+ reporter and lysosomal Ca2+-permeable channel

    Depositor

    Antony Galione

    Article

    Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

    Insert

    TPC2N2 (TPCN2 Human)
    

    Tags

    G-GECO1.2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PP_070_pAAV_CAG_LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2

    Plasmid

    #203228

    Purpose

    Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

    Depositor

    Adam Cohen

    Insert

    Membrane-localized optopatch for neuronal dendrites
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG dCas9-KRAB-2A-EGFP

    Plasmid

    #92396

    Purpose

    CAG-driven ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. Contains 2A-EGFP reporter. For targeted enhancer inactivation in chicken embryos.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    dCas9_KRAB_2A_EGFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMV_AA050

    Plasmid

    #216107

    Purpose

    gDNA expression for Cas9 with ChenF+E trRNA (guide only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    gDNA expression for Cas9 with ChenF+E trRNA
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PB533_2E12LI-sfGFP

    Plasmid

    #167527

    Purpose

    H3K27me3-mintbody expression vector for mammalian cells

    Depositor

    Hiroshi Kimura

    Article

    Tjalsma et al EMBO Rep. 2021 Feb 19:e51989. doi: 10.15

    Insert

    2E12LI-scFv
    

    Tags

    sfGFP

    Expression

    Mammalian

    Available Since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRDB_182

    Plasmid

    #216103

    Purpose

    CRISPRi, EF1a-driven dCas9-KRAB (ZIM3) (Cas only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    Cas9 [Sp]
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAd/CMV/V5-Clasp2α

    Plasmid

    #89763

    Purpose

    Adenovirus destination vector for transient expression of human wild-type EGFP-CLASP2α

    Depositor

    Torsten Wittmann

    Article

    Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

    Insert

    CLASP2α (CLASP2 Human)
    

    Use

    Adenoviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRDB_054

    Plasmid

    #216096

    Purpose

    All-in-one, knockout, delivers Cas12a & gRNA

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    Cas12a [EnAs]
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge