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Plasmid#231865PurposeBacterial expression of N-terminally 6His tagged Gcn4 Swap2DepositorInsertGcn4 Swap2
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 F124I
Plasmid#231864PurposeBacterial expression of N-terminally 6His tagged Gcn4 F124IDepositorInsertGcn4
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationF124IPromoterT7Available sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 Swap3
Plasmid#231860PurposeBacterial expression of N-terminally 6His tagged Gcn4 Swap3DepositorInsertGcn4 Swap3
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 WT 44mer
Plasmid#231856PurposeBacterial expression of N-terminally 6His tagged Gcn4 WT 44merDepositorInsertGcn4 44mer
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_FL Gcn4
Plasmid#231855PurposeBacterial expression of N-terminally 6His tagged FL Gcn4DepositorInsertGcn4
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMK195
Plasmid#226818PurposeIntegrates KanR into NS2, 1250 bp homology, two homologous HIP1 sites in each of the right and left homology armsDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDL1403
Plasmid#226635PurposeThis plasmid induces natural competence in classical biotype Vibrio cholerae and expresses the genes tfox and qstR.DepositorInsertstfox
qstR
UseTagsExpressionMutationPromoterAvailable sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVP293
Plasmid#222062PurposeGreenGate CD bridge module containing filler sequence, backbone domesticated of Esp3I sites.DepositorInsertFiller
UseSynthetic Biology; Greengate compatible cloning v…TagsExpressionMutationBackbone Esp3i sites have been domesticatedPromoterAvailable sinceDec. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVP294
Plasmid#222063PurposeGreenGate DE bridge module containing filler sequence, backbone domesticated of Esp3I sites.DepositorInsertFiller
UseSynthetic Biology; Greengate compatible cloning v…TagsExpressionMutationBackbone Esp3i sites have been domesticatedPromoterAvailable sinceDec. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVP076
Plasmid#222028PurposeEmpty spectinomycin resistant GreenGate destination vector based on pGGP-AG. Domesticated of Esp3I sites in the backbone and chloramphenicol resistance gene.DepositorTypeEmpty backboneUseSynthetic Biology; Greengate compatible cloning v…TagsExpressionMutationAll Esp3i sites domesticatedPromoterAvailable sinceDec. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVP077
Plasmid#222029PurposeEmpty Kanamycin resistant GreenGate destination vector based on pGGP-AG. Domesticated of Esp3I sites in the backbone and chloramphenicol resistance gene.DepositorTypeEmpty backboneUseSynthetic Biology; Greengate compatible cloning v…TagsExpressionMutationAll Esp3i sites domesticatedPromoterAvailable sinceDec. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSuccessor
Plasmid#228391Purposeintended to be co-transformed along with pDawn-Ag43 to generate successor strain (SS-GFP) for multipattern biofilm lithographyDepositorInsertpConst23100-sfGFP__pLambda-DHFR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2-hcar1-4-mKate2
Plasmid#226712Purposeoverexpression red fluorescent hcar1-4 protein in zebrafish larvaeDepositorInserthcar1-4
UseUnspecified; Zebrafish expressionTagsmKate2ExpressionMutationPromoterSP6Available sinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDUP
Plasmid#223515Purposecogfp under control of PtacDepositorArticleInsertCoGFP
UseTagsHis tagExpressionBacterialMutationPromoterPtac and Ptac and PtetAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAND-MCS
Plasmid#223514PurposePlasmid backbone with multiple cloning site for two genes in opposing directions (convergently transcribed)DepositorArticleTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPtac and PtetAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEARBOCS-v0-Gfap-GeneTRAP
Plasmid#196492PurposeTo KO and genetrap mouse GFAP simultaneouslyDepositorInsertsmCherry-CAAX
gRNA
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEARBOCS-v0-Vamp2-GeneTRAP
Plasmid#196493PurposeTo KO and genetrap mouse VAMP2 simultaneouslyDepositorInsertsmCherry-CAAX
gRNA
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEARBOCS-v0-Vamp2-TagIN-HA
Plasmid#196494PurposeTo tag endogenous mouse VAMP2 with HADepositorInsertsHA
gRNA
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pReporter_ZF1_mNeonGreen
Plasmid#213840PurposeFacilitates genomic integration of the reporter with upstream binding sites for ZF-1DepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pReporter_ZF2_mNeonGreen
Plasmid#213841PurposeFacilitates genomic integration of the reporter with upstream binding sites for ZF-2DepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNoRBS
Plasmid#218167PurposepET31b plasmid with short insert lacking ribosome binding site to increase 2',3'-cNMP levelsDepositorInsertShort insert
UseTagsExpressionBacterialMutationPromoterT7Available sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLBD16-YFP-GW
Plasmid#209369PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis LBD16 promoter driving expression of N-terminal YFP fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCR-YFP-GW
Plasmid#209366PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis SCR promoter driving expression of N-terminal YFP fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWOL-YFP-GW
Plasmid#209365PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis WOL promoter driving expression of N-terminal YFP fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pADH1-YFP-GW
Plasmid#209364PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis ADH1 promoter driving expression of N-terminal YFP fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCOBL-YFP-GW
Plasmid#209368PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis COBL promoter driving expression of N-terminal YFP fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAB1-YFP-GW
Plasmid#209363PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis CAB1 promoter driving expression of N-terminal YFP fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-eIF4E-TwStrep
Plasmid#208638Purposefor E. coli expression of C-ter Twin Strep tagged human eIF4E (ENST00000450253)DepositorInserthuman eIF4E (ENST00000450253) (EIF4E Human)
UseTags6xHis and Twin StrepExpressionBacterialMutationPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-mEGFP(A206K)-TwStrep
Plasmid#208622Purposefor E. coli expression of C-ter Twin Strep tagged mEGFP(A206K), EGFP cloned from pEGFP-C1, A206K introduced by seamless (Gibson) cloneDepositorInsertmEGFP(A206K)
UseTags6xHis and Twin StrepExpressionBacterialMutation(compared with pEGFP-C1, protein level) A206KPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
mTurquoise2-Scarlet-I
Plasmid#193558PurposeExpresses mTurquoise2-mScarlet-I in mammalian cells.DepositorInsertmTurquoise2-mScarlet-I
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only