We narrowed to 2,785 results for: ALC

1. pET-51b(+)-Caprola_15

   Plasmid

   #194676

   Purpose

   Protein production of the split HaloTag based calcium recorder Caprola_15 in bacteria

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_15
   

   Use

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA5/FRT_CMV_NES-Caprola_04-mEGFP

   Plasmid

   #194682

   Purpose

   CMV driven expression of the calcium recorder Caprola_04 in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_04-mEGFP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cav2.2e[a10, 18a, Δ24a, 31a, 37b, 46]

   Plasmid

   #26568

   Depositor

   Diane Lipscombe

   Article

   Pan et al J Neurosci. 2000 Jul 1. 20(13):4769-75.

   Insert

   calcium channel alpha-1B subunit (Cacna1b Rat)
   

   Use

   Tags

   6XHis and V5

   Expression

   Mammalian

   Mutation

   contains exons a10, 18a, 31a, 37b, and 46. Del…

   Promoter

   Available since

   Nov. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pCDNA5/FRT_CMV_NES-Caprola_06-mEGFP

   Plasmid

   #194684

   Purpose

   CMV driven expression of the calcium recorder Caprola_06 in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_06-mEGFP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA-SCW-HHL

   Plasmid

   #190634

   Purpose

   Mammalian expression of genetically encoded calcium indicator SCW-HHL

   Depositor

   Robert Campbell

   Article

   Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

   Insert

   SCW-HHL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223 LC3A G120A

   Plasmid

   #123070

   Purpose

   Gateway entry clone encoding human MAP1LC3A G120A

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
   

   Use

   Gateway entry vector / entry clone

   Tags

   Expression

   Mutation

   Glycine 120 to Alanine

   Promoter

   Available since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cav2.2e[Δa10, Δ18a, Δ24a, 31a, 37b, 46]

   Plasmid

   #26570

   Depositor

   Diane Lipscombe

   Article

   Lin et al Neuron. 1997 Jan . 18(1):153-66.

   Insert

   calcium channel alpha-1B subunit (Cacna1b Rat)
   

   Use

   Tags

   6XHis and V5

   Expression

   Mammalian

   Mutation

   Deletion of exons a10, 18a, and 24a. Contains …

   Promoter

   Available since

   Nov. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

8. HBT-CPK32-Flag

   Plasmid

   #107432

   Purpose

   For Arabidopsis mesophyll protoplast transient expression assay

   Depositor

   Jen Sheen

   Article

   Liu et al Nature. 2017 May 18;545(7654):311-316. d

   Insert

   CPK32 (CPK32 Mustard Weed)
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Bxb1_jRCaMP1b

   Plasmid

   #183758

   Purpose

   Bxb1-specific donor plasmid for intergrating a transcription unit for expression of jRCaMP1b calcium reporter

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   jRCaMP1b
   

   Use

   Tags

   NES

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGP-CMV-GCaMP3-44

    Plasmid

    #91780

    Purpose

    Mammalian expression of low affinity calcium-sensing fluorescent protein

    Depositor

    Douglas Kim, GENIE Project

    Article

    de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

    Insert

    GCaMP3-44
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSET a sfGCaMP6s-T78H

    Plasmid

    #100023

    Purpose

    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant containing T78H mutation

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    sfGCaMP6s-T78H
    

    Use

    Tags

    6x HIS tag and Xpress_EK tag

    Expression

    Bacterial

    Mutation

    cpEGFP replaced with superfolder cpGFP and T78H m…

    Promoter

    T7

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBAD-SCW-HHL

    Plasmid

    #190627

    Purpose

    Bacterial expression of genetically encoded calcium indicator SCW-HHL

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    SCW-HHL
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSG5-FLAG-CaMKKbeta rat RP deletion

    Plasmid

    #32452

    Depositor

    Anthony Means

    Article

    Anderson et al Cell Metab. 2008 May;7(5):377-88.

    Insert

    Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Deleted RP domain (amino acids 203-225 missing); …

    Promoter

    SV40

    Available since

    April 17, 2012

    Availability

    Academic Institutions and Nonprofits only

14. Lentiviral FLAG-CaMKKbeta S129D, S133D, S137D

    Plasmid

    #32460

    Depositor

    Anthony Means

    Article

    Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

    Insert

    Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    S129D, S133D, S137D

    Promoter

    human PGK

    Available since

    Feb. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pRSET a sfMatryoshCaMP6s

    Plasmid

    #100020

    Purpose

    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting superfolder cpGFP.

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    sfMatryoshCaMP6s
    

    Use

    Tags

    6x HIS tag and Xpress_EK tag

    Expression

    Bacterial

    Mutation

    cpEGFP replaced with superfolder cpGFP

    Promoter

    T7

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMSP1D1T2

    Plasmid

    #97328

    Purpose

    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include two additional T residues shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

    Depositor

    Michael Marty

    Article

    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

    Insert

    MSP1D1T2
    

    Use

    Tags

    6xHis - TEV

    Expression

    Bacterial

    Mutation

    Addition of two T residues after T25

    Promoter

    T7

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSG5-FLAG-CaMKKbeta rat S100A, S495A and S511A

    Plasmid

    #33320

    Depositor

    Anthony Means

    Article

    Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

    Insert

    Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S99A, S494A, S510A

    Promoter

    SV40

    Available since

    April 23, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pSG5-FLAG-CaMKK alpha with CaMKKbeta RP domain

    Plasmid

    #33331

    Depositor

    Anthony Means

    Article

    Green et al Cell Signal. 2011 Dec;23(12):2005-12. Ep

    Insert

    Calcium/Calmodulin dependent protein kinase kinase alpha (Camkk1 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    CaMKKalpha RP domain (167-189) was replaced with …

    Promoter

    SV40

    Available since

    April 10, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pLV[Exp]-Puro-CMV>{5-HT6-R_GECO1.1}

    Plasmid

    #232879

    Purpose

    Expresses the 5-HT6R coupled to the RGECO1 calcium sensor

    Depositor

    Paul DeCaen

    Article

    DeCaen et al J Cell Physiol. 2025 Apr;240(4):e70034.

    Insert

    5-Hydroxytryptamine Receptor 6 (HTR6 Human)
    

    Use

    Lentiviral

    Tags

    RGECO1.1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-DIO-GCaMP6f-WPRE

    Plasmid

    #216275

    Purpose

    AAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of green fluorescent calcium indicator GCaMP6f

    Depositor

    Guohong Cui

    Article

    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

    Insert

    GCaMP6f
    

    Use

    AAV and Cre/Lox

    Tags

    6XHIS and Xpress

    Expression

    Mutation

    Promoter

    human Synapsin 1 (hSyn)

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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