We narrowed to 14,031 results for: TIM

1. pAW8ENdeI-c3HA-8XDSR

   Plasmid

   #49871

   Purpose

   for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 8 copies of the core DSR motif into the S. pombe urg1 locus

   Depositor

   Tony Carr

   Article

   Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   3XHA

   Available Since

   Dec. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAW8ENdeI-c3HA-4XDSR

   Plasmid

   #49869

   Purpose

   for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 4 copies of the core DSR motif into the S. pombe urg1 locus

   Depositor

   Tony Carr

   Article

   Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   3XHA

   Available Since

   Dec. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFA6a-link-yomEmerald-CaURA3

   Plasmid

   #44890

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized mEmerald with CaUra3 selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yomEmerald
   

   Expression

   Yeast

   Available Since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA6a-link-yoMaxGFP-CaURA3

   Plasmid

   #44875

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized MaxGFP with CaUra3 selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoMaxGFP
   

   Expression

   Yeast

   Available Since

   May 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-link-yoMaxGFP-SpHis5

   Plasmid

   #44840

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized MaxGFP with SpHis5 selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoMaxGFP
   

   Expression

   Yeast

   Available Since

   May 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. -24lacZ

   Plasmid

   #25422

   Depositor

   David J. Goldhamer

   Article

   Chen et al Dev Dyn. 2001 Jul . 221(3):274-88.

   Insert

   Myo D 5' flanking sequence (MYOD1 Human)
   

   Use

   Reporter construct

   Tags

   lacZ

   Expression

   Mammalian

   Available Since

   June 29, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pRmHa3-dKDM4A-H195A-HA2FL2

   Plasmid

   #20235

   Depositor

   Jerry Workman

   Article

   Lin et al Mol Cell. 2008 Dec 5. 32(5):696-706.

   Insert

   dKDM4A (Kdm4A Fly)
   

   Tags

   FLAG2 and HA2

   Expression

   Insect

   Mutation

   changed His 195 to Ala

   Available Since

   March 19, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pRSET FLII282Pglu-4m

   Plasmid

   #13564

   Depositor

   Wolf Frommer

   Article

   Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

   Insert

   FLIPglu F16A T282S-ECFP-N283R
   

   Tags

   Citrine

   Expression

   Bacterial

   Mutation

   F16A, ECFP insert in binding protein

   Available Since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. Halo-KDEL

   Plasmid

   #124316

   Purpose

   Expresses ER specific HaloTag protein

   Depositor

   Jin Wang

   Article

   Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

   Insert

   Halo tag
   

   Tags

   KDEL Seq

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. eMagB-iRFP-Mito

    Plasmid

    #162250

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    eMagB-iRFP-OMP25
    

    Expression

    Mammalian

    Promoter

    beta-actin promoter

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. ER-mCherry-eMagA

    Plasmid

    #162248

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    CytP450 ER targeting sequencel-mCherry-eMagA
    

    Expression

    Mammalian

    Promoter

    beta-actin promoter

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSIRV-NFAT-eGFP

    Plasmid

    #118031

    Purpose

    Fluorescent reporter for NFAT activation

    Depositor

    Peter Steinberger

    Article

    Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

    Insert

    eGFP
    

    Use

    Retroviral

    Promoter

    minimal promoter with NFAT binding site

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. LT3GEPIR

    Plasmid

    #111177

    Purpose

    Tet-ON miR-E (miR-30 variant)-based RNAi

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMJ915

    Plasmid

    #69090

    Purpose

    Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLS

    Depositor

    Jennifer Doudna

    Article

    Lin et al Elife. 2014 Dec 15;3:e04766. doi: 10.755

    Insert

    cas9
    

    Tags

    MBP, hexa His, and two SV40 NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSIRV-AP-1-mCherry

    Plasmid

    #118095

    Purpose

    Fluorescent reporter for AP-1 activation

    Depositor

    Peter Steinberger

    Article

    Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

    Insert

    mCherry
    

    Use

    Retroviral

    Promoter

    minimal promoter with AP-1 responsive element

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Halo-MTS

    Plasmid

    #124315

    Purpose

    Expresses mitochondria specific HaloTag protein

    Depositor

    Jin Wang

    Article

    Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

    Insert

    Halo tag
    

    Tags

    Mito specific seq

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXPR_502

    Plasmid

    #96923

    Purpose

    for CRISPRa, lentiviral expression of gRNA scaffold using activator P65 HSF

    Depositor

    John Doench, David Root

    Article

    Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRDA_174

    Plasmid

    #136476

    Purpose

    Lentiviral expression of EnAsCas12a

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

    Insert

    EnAsCas12a
    

    Use

    CRISPR and Lentiviral

    Promoter

    EF1a

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pICH47742:: pRPS5a-PE2max-NC::tHSP

    Plasmid

    #227694

    Purpose

    Golden Gate level 1, position 2, PE2max-NC expression cassette for Arabidopsis.

    Depositor

    Jae-Yean Kim

    Article

    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

    Insert

    PE2max-NC being driven by pRPS5a and terminated by tHSP
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    pRPS5a

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMOS015: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

    Plasmid

    #163059

    Purpose

    mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    mito-roGFP2-Orp1
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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