Skip to main content
Addgene
Showing: 5241 - 5280 of 17176 results

  3. PDG459 V3

    Plasmid
    #226958
    Purpose
    CBh-SpCas9-2A-Puro, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226958-plasmid-map-sequence-id-450823

    Enlarge

  4. SaCas9 GFP V3

    Plasmid
    #226962
    Purpose
    CBh-SaCas9-2A-GFP, and hU6-sgRNA (Sa) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226962-plasmid-map-sequence-id-450821

    Enlarge

  5. Pdg459-eSp(1.1) V3

    Plasmid
    #226965
    Purpose
    CBh-eSpCas9(1.1)-2A-Puro, and 2X hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226965-plasmid-map-sequence-id-450820

    Enlarge

  10. pLV-CS-060

    Plasmid
    #197133
    Purpose
    CRISPRa reporter cloning backbone and MCP-p65-HSF1-2A-Puro expression
    Depositor
    Nozomu Yachie
    Article
    Ishiguro et al bioRxiv 2023.01.18.524633
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EGFP and mCherry
    Expression
    Mammalian
    Mutation
    MCP(N55K)
    Promoter
    Available since
    May 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    197133-plasmid-map-sequence-id-392596

    Enlarge

  18. PEA1-Nuclease

    Plasmid
    #176528
    Purpose
    Delivers all prime editing nuclease components in a single plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    GC to CA point mutation in SpCas9 to restore nucl…
    Promoter
    CbH for Cas9, hU6 for gRNAs
    Available since
    Dec. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176528-plasmid-map-sequence-id-350557

    Enlarge

  28. pLenti-CMV-TIRAP-cGASΔN-HA

    Plasmid
    #130921
    Purpose
    Expresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the TIRAP N terminus (aa1-85); Puromycin selection marker
    Depositor
    Jonathan Kagan
    Article
    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d
    Insert
    TIRAP-ΔNcGAS
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    cGAS N terminus (aa1-159) replaced with the N ter…
    Promoter
    CMV
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    130921-plasmid-map-sequence-id-255048

    Enlarge

  29. pLenti-CMV-Fyn-cGASΔN-HA

    Plasmid
    #130922
    Purpose
    Expresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the Fyn dual acylation motif (aa1-16); Puromycin selection marker
    Depositor
    Jonathan Kagan
    Article
    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d
    Insert
    Fyn-ΔNcGAS
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    cGAS N terminus (aa1-159) replaced with the dual …
    Promoter
    CMV
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    130922-plasmid-map-sequence-id-255037

    Enlarge

Showing: 5241 - 5280 of 17176 results