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pEGFP-WRAP53beta-WD40domain

Plasmid

#64678

Purpose

Expresses the WD40 domain of WRAP53beta

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

Wd40-encoding RNA antisense to P53 (WRAP53 Human)

Tags

GFP

Expression

Mammalian

Mutation

containing only WD40 domain of WRAP53beta

Promoter

CMV

Available Since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pTet02

Plasmid

#78284

Purpose

mRFP1 expressed behind an ATc-inducible construct

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

Tet inducible mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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Mouse c-myc (exon I)

Plasmid

#8446

Depositor

Frederic Mushinski

Article

Weissinger et al Mol Cell Biol 1993 Apr;13(4):2578-85.

Insert

Mouse c-myc (exon I) (Myc Mouse)

Expression

Bacterial

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pRK5-HA CASTOR2 ACT2

Plasmid

#84494

Purpose

overexpression of CASTOR2 ACT2 domain (aa 171-end)

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR2 ACT2 (GATSL2 Human)

Tags

HA

Expression

Mammalian

Mutation

171-last amino acid

Promoter

CMV

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-Flag CASTOR2 ACT1

Plasmid

#84493

Purpose

overexpression of CASTOR2 ACT1 domain (aa 1-171)

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR2 ACT1 (GATSL2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

1-171 amino acid

Promoter

CMV

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pmTol-InsUAS-6MTnlstdTomato2A-Dusp6DM-CC

Plasmid

#67687

Purpose

UAS-driven 6xMyc nuclear localized tdTomato-2A-activated Dusp6 in minTol backbone; also with cry:cfp cassette

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3796-38

Insert

dusp6 (dusp6 Zebrafish)

Tags

6xmyc-NLS-tdTomato-2A

Mutation

S159A, S196A

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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kank3_pJet1.2

Plasmid

#121981

Purpose

for generating kank3 riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank3 (kank3 Zebrafish)

Use

Cloning vector

Promoter

T7

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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kank4_pJet1.2

Plasmid

#121982

Purpose

for generating kank4 riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank4 (kank4 Zebrafish)

Use

Cloning vector

Promoter

T7

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Actin-C-18

Plasmid

#55400

Purpose

Localization: Actin, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Actin (ACTB Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA3.1(-) + SLNCR1 _462-572 (conserved sequence)

Plasmid

#86818

Purpose

mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

LINC00673 (LINC00673 Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 25, 2017

Availability

Academic Institutions and Nonprofits only

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CSII-IDR2-AGMpygTRIM5-FOS

Plasmid

#79064

Purpose

Express African green monkey pygerythrus TRIM5alpha in mammalan cells

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5alpha(African green monkey pygerythrus)-FOS

Tags

OneSTrEP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-Hook3 I136A, I139A, M142A

Plasmid

#74617

Purpose

Expresses a triple mutant truncation of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-239, I136A, I139A, M142A (HOOK3 Human)

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Mutation

D102A, E108A

Promoter

T7

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pBV-Luc mut MBS3+4

Plasmid

#16569

Depositor

Bert Vogelstein

Article

Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

Insert

c-MYC binding sites (MYC Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant MBS3+4

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
pBV-Luc mut MBS1

Plasmid

#16566

Depositor

Bert Vogelstein

Article

Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

Insert

c-MYC binding sites (MYC Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant MBS1

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
pBV-Luc mut MBS2

Plasmid

#16567

Depositor

Bert Vogelstein

Article

Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

Insert

c-MYC binding sites (MYC Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant MBS2

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
pBV-Luc mut MBS1-3

Plasmid

#16568

Depositor

Bert Vogelstein

Article

Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

Insert

c-MYC binding sites (MYC Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant MBS1-3

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
pBV-Luc mut MBS4

Plasmid

#16570

Depositor

Bert Vogelstein

Article

Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

Insert

c-MYC binding sites (MYC Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant MBS4

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
pBabe hygro human Frataxin

Plasmid

#11504

Depositor

Ronald Kahn

Article

Ristow et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

Insert

Frataxin (FXN Human)

Use

Retroviral

Expression

Mammalian

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only
pBlue-hFX(AA88)-HA

Plasmid

#15402

Depositor

Michael Ristow

Article

Dhe-Paganon et al J Biol Chem. 2000 Oct 6. 275(40):30753-6

Insert

human frataxin cDNA (FXN Human)

Tags

AU1, HA, and HIS

Expression

Bacterial

Mutation

contains cDNA coding sequence for amino acids 88-…

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pBabe neo human Frataxin

Plasmid

#11503

Depositor

Ronald Kahn

Article

Ristow et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

Insert

Frataxin (FXN Human)

Use

Retroviral

Expression

Mammalian

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only