We narrowed to 5,678 results for: KRAS

Showing: 2621 - 2640 of 5678 results

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pcDNA5FRT/TO-p47-Strep-HA

Plasmid

#113475

Purpose

Expression of human p47 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

p47 (NSFL1C Human)

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pHIV-SapI-LacZ

Plasmid

#237996

Purpose

Expression plasmid for transfer of complete TCR library from pGGA backbone

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Type

Empty backbone

Use

Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry-P2AT2A-EGFP-BRD4-NUT

Plasmid

#238281

Purpose

For overexpression of mCherry-P2AT2A-EGFP-BRD4-NUT

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry-P2AT2A-EGFP-BRD4-NUT

Expression

Mammalian

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCDF-mTET1

Plasmid

#81052

Purpose

bacterial expression of murine TET1

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TET1 (Tet1 Mouse)

Tags

6HIS

Expression

Bacterial

Promoter

T7

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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WN10150

Plasmid

#80443

Purpose

Expresses dead (908A) AsCpf1

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Insert

nuclease dead (908A) AsCpf1

Tags

NLS-3xHA

Expression

Mammalian

Mutation

nuclease dead (D908A)

Available Since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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3XFLAG_GAPDH in pCW57_tTA_Blast

Plasmid

#154211

Purpose

Dox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of GAPDH

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

GAPDH (GAPDH Human)

Use

Lentiviral

Tags

3X FLAG

Expression

Mammalian

Promoter

TRE repeats (dox off)

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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p2T-cmv-SpCas9-TadCBEd-BlastR

Plasmid

#193848

Purpose

Express TadCBEd in mammalian cells with BlastR selection, Tol2 integration

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadCBEd

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgGrin2b

Plasmid

#124864

Purpose

Mutagenesis of Grin2b

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Grin2b (Grin2b Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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peGFP C3-SPG20-PAAA

Plasmid

#218581

Purpose

Expresses GFP-tagged PPAY motif mutant (PAAA) of human SPG20 in mammalian cells; this mutant does not interact with ITCH

Depositor

Hemmo Meyer

Article

Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

Insert

SPG20 (SPART Human)

Tags

eGFP

Expression

Mammalian

Mutation

changed Proline 172 to Alanine and Tyrosine 174 t…

Promoter

CMV

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgGrin2b

Plasmid

#124851

Purpose

Mutagenesis of Grin2b

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Grin2b (Grin2b Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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PET-B-Sniper SpCas9-NLS-6xHis

Plasmid

#207377

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-Sniper SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

F539S, M763I, K890N, amino acids 1005-1013 replac…

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pGFP-HBO1-G485A pc2201

Plasmid

#167570

Purpose

The human HBO1 is fused to C-terminal GFP of EGFP-C1 (Clontech)

Depositor

Cristina Cardoso

Article

Heinz et al Epigenetics Chromatin. 2019 Mar 14;12(1)

Insert

histone acetyltransferase 1 (KAT7 Human)

Tags

GFP

Expression

Mammalian

Mutation

G485A

Promoter

CMV

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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AA063

Plasmid

#216020

Purpose

Fragmid fragment: (Cas protein) nickase Cas for prime editing

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

nCas9-SpRY (H840A)_v1.1 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA183

Plasmid

#216025

Purpose

Fragmid fragment: (Cas protein) for prime editing; R221K;N394K for increased efficiency

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

nCas9 (H840A)_v2.1 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA062

Plasmid

#216019

Purpose

Fragmid fragment: (Cas protein) nickase Cas for prime editing

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

nCas9-SpG (H840A)_v1.1 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA060

Plasmid

#216017

Purpose

Fragmid fragment: (Cas protein) nickase Cas for prime editing

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

nCas9 (H840A)_v1.1 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-tagBFP-2A-ICP0-FxE

Plasmid

#235539

Purpose

To express tagBFP-2A-ICP0-FXE (ICP0 ∆106-149)

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

ICP0-FxE

Use

Lentiviral

Mutation

Deletion of the RING domain, ∆106-149.

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 p37deltaSEP

Plasmid

#113501

Purpose

Bacterial expression of GST-tagged p37 lacking the SEP domain

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p37 (Ubxn2b Mouse)

Tags

GST-PreScission protease site

Expression

Bacterial

Mutation

delta 140-206

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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PET-Sniper SpCas9-NLS-6xHis

Plasmid

#207374

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

Sniper SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

F539S, M763I, K890N

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR221-TCF7

Plasmid

#79498

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human TCF7 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

TCF7 (TCF7 Human)

Use

Multisite gateway entry clone

Promoter

no Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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