We narrowed to 2,783 results for: aen

1. pDEST40-2XFL-Src-K298M-Y419F-Y530F

   Plasmid

   #140321

   Purpose

   Expresses Flag-tagged human Src-K298M-Y419F-Y530F in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src mutant (SRC Human)
   

   Use

   Tags

   2X Flag

   Expression

   Mammalian

   Mutation

   K298M-Y419F-Y530F

   Promoter

   CMV

   Available since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKM-U6-reRNA-hsHEKsite_1_3

   Plasmid

   #205638

   Purpose

   reRNA guide targeting HEKsite_1_3

   Depositor

   Gerald Schwank

   Article

   Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

   Insert

   reRNA-HEKsite_1_3 (EPHA3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOD2046-bwmDEG

   Plasmid

   #89367

   Purpose

   MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pmyo-3 (body wall muscle specific).

   Depositor

   Karen Oegema

   Article

   Wang et al Development. 2017 Jun 15. pii: dev.15009

   Insert

   vhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)
   

   Use

   Targeting vector for mos1 transposon mediated sin…

   Tags

   vhhGFP4 (GFP nanobody)

   Expression

   Mutation

   Promoter

   Pmyo-3

   Available since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. hum alpha ENAC promoter a25-a23

   Plasmid

   #83427

   Purpose

   promoter reporter construct to test 5' flanking sequences

   Depositor

   Christie Thomas

   Article

   Sayegh et al J Biol Chem. 1999 Apr 30;274(18):12431-7

   Insert

   human aENaC gene 5' flanking seq + 55 nt of the 5' UTR for exon 1A (SCNN1A Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mutation

   Promoter

   alpha ENAC

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSicoR p53

   Plasmid

   #12090

   Depositor

   Tyler Jacks

   Article

   Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

   Insert

   p53 shRNA (Trp53 Mouse)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   EGFP is expressed from this plasmid as a marker, …

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. Stx5S-GFP

   Plasmid

   #209847

   Purpose

   Expresses Stx5S-GFP in mammalian cells

   Depositor

   Geert van den Bogaart

   Article

   Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

   Insert

   STX5 (STX5 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Lacks amino acids 1-54, encodes short isoform of …

   Promoter

   CMV

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCC324

   Plasmid

   #110165

   Purpose

   miniMos vector expresses ERKKTR-mClover and mCherry-H2B in C. elegans VPCs; request with plasmids pCC335 and pCC336 for unphosphorylated and phosphomimetic controls (Contains Neomycin resistance)

   Depositor

   Iva Greenwald

   Article

   de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.

   Insert

   ERK-KTR-mClover-T2A-mCherry-H2B
   

   Use

   Minimos transposon

   Tags

   mCherry and mClover

   Expression

   Worm

   Mutation

   R61K change made in ERK-KTR CDS

   Promoter

   lin-31 promoter/enhancer

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOD2048-csnDEG

   Plasmid

   #89368

   Purpose

   MosSCI targeting vector expressing a fusion between a GFP nanobody and ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression controlled by Posm-6 (ciliated sensory neuron specific)

   Depositor

   Karen Oegema

   Article

   Wang et al Development. 2017 Jun 15. pii: dev.15009

   Insert

   vhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)
   

   Use

   Targeting vector for mos1 transposon mediated sin…

   Tags

   vhhGFP4 (GFP nanobody)

   Expression

   Mutation

   Promoter

   Posm-6

   Available since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pGL4.15_pSOX17

   Plasmid

   #195496

   Purpose

   Firefly luciferase promoter expression vector driven only by the promoter element of human SOX17

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   Promoter region of SOX17 (SOX17 Human)
   

   Use

   Tags

   luc2-hPEST

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pETM6-4F2-mCherry

    Plasmid

    #73421

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 4F2 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P4F2 (orthogonal T7-lac variant)

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSicoR Dnmt1

    Plasmid

    #12166

    Depositor

    Tyler Jacks

    Article

    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

    Insert

    Dnmt1 shRNA (Dnmt1 Mouse)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    EGFP is expressed from this plasmid as a marker, …

    Promoter

    Available since

    June 30, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pFastBac_Dual-GST-TEV-SINTBAD-EGFP (codon optimized Sf9)

    Plasmid

    #198035

    Purpose

    Used for the expression and purification of EGFP labelled SINTBAD (SMC Internal No. 1613)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    SINTBAD (TBKBP1 Human)
    

    Use

    Tags

    EGFP and GST-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pv6_MECP2_MBD

    Plasmid

    #179407

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    MECP2_MBD (Mecp2 Mouse)
    

    Use

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. p029 End-1::EnR

    Plasmid

    #11029

    Depositor

    Ramesh Shivdasani

    Article

    Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

    Insert

    End1::EnR (end-1, en END-1 is from C. Elegans, EnR is from Drophila)
    

    Use

    Xenopus, mammalian, avian, and zebrafish

    Tags

    Expression

    Mutation

    Dominant inhibitory GATA-binding protein End1::En…

    Promoter

    Available since

    Dec. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETM6-3F2-mCherry

    Plasmid

    #73418

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3F2.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 3F2 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P3F2 (orthogonal T7-lac variant)

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pETM6-3A2-mCherry

    Plasmid

    #73425

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3A2.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 3A2 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P3A2 (orthogonal T7-lac variant)

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pETM6-5F5-mCherry

    Plasmid

    #73422

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 5F5.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 5F5 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P5F5 (orthogonal T7-lac variant)

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKM-U6-reRNA-hsRUNX

    Plasmid

    #205639

    Purpose

    reRNA guide targeting Homo sapiens RUNX

    Depositor

    Gerald Schwank

    Article

    Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

    Insert

    reRNA-hsRUNX (RUNX1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDV18 [punc-47::SNG-1::CRY2olig(535)]

    Plasmid

    #197598

    Purpose

    Expression of SNG-1::CRY2olig(535) in GABAergic motor neurons of C. elegans

    Depositor

    Alexander Gottschalk

    Article

    Vettkotter et al Nat Commun. 2022 Dec 19;13(1):7827. doi:

    Insert

    Synaptogyrin-CRY2 (E490G + residues 1-535) (CRY2, sng-1 Nematode, Mustard Weed)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    E490G

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDV12 [psng-1::SNG-1::CRY2olig(535)]

    Plasmid

    #197596

    Purpose

    Pan-neuronal expression of SNG-1::CRY2olig(535) in neurons of C. elegans

    Depositor

    Alexander Gottschalk

    Article

    Vettkotter et al Nat Commun. 2022 Dec 19;13(1):7827. doi:

    Insert

    Synaptogyrin-CRY2 (E490G + residues 1-535) (CRY2, sng-1 Nematode, Mustard Weed)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    E490G

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge